|Application / Reactivity||Mouse (Murine)||Human|
|ELISA||3 Antibodies||22 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||2 Antibodies||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||3 Antibodies||3 Antibodies|
|Western Blotting (WB)||12 Antibodies||42 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Antigen||Protein Phosphatase 1, Regulatory Subunit 13B (PPP1R13B) Antibodies|
|Epitope||Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine) Alternatives|
|Conjugate||This PPP1R13B antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|5 references available|
|Supplier||Log in to see|
Product Details anti-PPP1R13B AntibodyTarget Details PPP1R13B Application Details Handling References for anti-PPP1R13B Antibody (ABIN401394) Images
|Specificity||This antibody is directed against ASPP1.|
Species reactivity (expected):Mouse.
Species reactivity (tested):Human.
|Immunogen||Synthetic peptide corresponding to an internal sequence of human ASPP1|
Target Details PPP1R13BProduct Details anti-PPP1R13B Antibody Application Details Handling References for anti-PPP1R13B Antibody (ABIN401394) Images back to top
|Alternative Name||PPP1R13B (PPP1R13B Antibody Abstract)|
|Background||ASPP (ankyrin-repeat-, SH3-domain- proline-rich-region protein) proteins (ASPP1, ASPP2 and iASPP) represent a new family of p53 binding proteins. ASPP1 and ASPP2 bind and enhance p53-mediated apoptosis. In contrast, iASPP functionally inactivates p53. ASPPs may also regulate p63- and p73-mediated apoptosis. Both ASPP1 and 2 directly interact with p53 and specifically enhance the apoptotic function of p53 by stimulating its DNA binding and transactivation function on promoters of pro-apoptotic genes, such as Bax and PIG-3. Not all cell cycle arrest genes are affected, such as p21. Interestingly, expression of ASPP is frequently down-regulated in human breast carcinomas expressing wild-type p53 but not mutant p53. Therefore, ASPP might regulate the tumor suppression function of p53 in vivo.Synonyms: ASPP1, Apoptosis-stimulating of p53 protein 1, KIAA0771, Protein phosphatase 1 regulatory subunit 13B|
Application DetailsProduct Details anti-PPP1R13B Antibody Target Details PPP1R13B Handling References for anti-PPP1R13B Antibody (ABIN401394) Images back to top
ELISA: 1/2,500 - 1/10,000. Western Blot: 1/500 - 1/3,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-PPP1R13B Antibody Target Details PPP1R13B Application Details References for anti-PPP1R13B Antibody (ABIN401394) Images back to top
|Concentration||1.3 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
References for anti-PPP1R13B Antibody (ABIN401394)Product Details anti-PPP1R13B Antibody Target Details PPP1R13B Application Details Handling Images back to top
Trigiante, Lu: "ASPP [corrected] and cancer." in: Nature reviews. Cancer, Vol. 6, Issue 3, pp. 217-26, 2006 (PubMed).
Liu, Lu, Zhong: "ASPP--Apoptotic specific regulator of p53." in: Biochimica et biophysica acta, Vol. 1756, Issue 1, pp. 77-80, 2005 (PubMed).
Liu, Lu, Zhang et al.: "Downregulated mRNA expression of ASPP and the hypermethylation of the 5'-untranslated region in cancer cell lines retaining wild-type p53." in: FEBS letters, Vol. 579, Issue 7, pp. 1587-90, 2005 (PubMed).
Slee, Lu: "The ASPP family: deciding between life and death after DNA damage." in: Toxicology letters, Vol. 139, Issue 2-3, pp. 81-7, 2003 (PubMed).
Samuels-Lev, OConnor, Bergamaschi et al.: "ASPP proteins specifically stimulate the apoptotic function of p53." in: Molecular cell, Vol. 8, Issue 4, pp. 781-94, 2001 (PubMed).
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