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anti-Human RRM2B antibody for Immunoprecipitation

Recommended RRM2B Antibody (supplied by: Log in to see )

Antigen
Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) Antibodies
  • MTDPS8A
  • MTDPS8B
  • P53R2
  • p53r2
  • p53R2
  • RRM2B
Epitope
N-Term
17
16
15
12
11
3
3
2
2
2
2
1
1
1
Reactivity
Human
84
37
35
2
1
Host
Rabbit
79
5
Clonality
Polyclonal
Conjugate
This RRM2B antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
65
39
15
11
11
7
3
2
1
Supplier
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Catalog No. ABIN964915
$ 369.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.00089235755 ABIN1104077 IF IP IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.00089235755 ABIN964916 IP IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
0.00089235755 ABIN2354451 IF IP IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal

Similar anti-RRM2B Antibodies

Application / Reactivity Human
ELISA 40 Antibodies
Enzyme Immunoassay (EIA) 2 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 7 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 11 Antibodies
Immunohistochemistry (IHC) 16 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 11 Antibodies
Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 66 Antibodies

General

Antigen Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) Antibodies
Epitope N-Term
(17), (16), (15), (12), (11), (3), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human
(84), (37), (35), (2), (1)
Host Rabbit
(79), (5)
Clonality
Polyclonal
Conjugate This RRM2B antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Immunoprecipitation (IP), Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(65), (39), (15), (11), (11), (7), (3), (2), (1)
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Product Details anti-RRM2B Antibody

Target Details RRM2B Application Details Handling Images
Specificity Anti-Human RRM2B/p53R2 is directed against RRM2B and reacts with the RRM2B from human tissues. Based on sequence we expect this antibody to react as well with RRM2B from other species. Based on 100% homology to the immunizing sequence, one may expect reactivity to chimpanzee and orangutan, based on 93% homology, reactivity to macaque, and 92% to pig and Drosophila.
Characteristics RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-iron-carboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.
Purification affinity purified
Sterility Sterile filtered
Immunogen Anti-Human RRM2B/p53R2 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein. A residue of cysteine was added to facilitate coupling.
Immunogen Type: Peptide
Isotype IgG

Target Details RRM2B

Product Details anti-RRM2B Antibody Application Details Handling Images back to top
Antigen
Alternative Name RRM2B p53R2 (RRM2B Antibody Abstract)
Background RRM2B/p53-R2, or p53-inducible ribonucleotide reductase small subunit 2-like protein, is a member of a broad superfamily of ferritin-like di-iron-carboxylate proteins. The RRM2B protein is an enzyme that catalyzes the conversion of ribonucleotides to deoxyribonucleotides that are essential for DNA synthesis, and is found in all eukaryotes. RRM2B plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2. It contains an iron-tyrosyl free radical center required for catalysis, and forms an active ribonucleotide reductase (RNR) complex with RRM1 which is expressed both in resting and proliferating cells in response to DNA damage. It is a heterotetramer with a large (RRM1) subunit, and interacts with p53/TP53. The interaction with RRM1 occurs in response to DNA damage and results in its translocation from cytoplasm to nucleus. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus, and expressed in epithelial dysplasias and squamous cell carcinoma. Defects in RRM2B are the cause of encephalomyopathic mitochondrial depletion syndrome with renal tubulopathy (EMDSRT). Mitochondrial DNA depletion syndrome (MDS) is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. The encephalomyopathic form with renal tubulopathy is presented with various combinations of hypotonia, tubulopathy, seizures, respiratory distress, diarrhea, and lactic acidosis.
Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B; Ribonucleotide Reductase, R2/beta subunit; small subunit of p53 (MIM 191170)-inducible ribonucleotide reductase; RIR2B; RNRR2, TP53-inducible ribonucleotide reductase M2 B.
Gene ID 50484, 42544136
UniProt Q7LG56
Pathways p53 Signaling

Application Details

Product Details anti-RRM2B Antibody Target Details RRM2B Handling Images back to top
Application Notes RRM2B/p53R2 antibody is suitable for IP, IHC, IF, ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RRM2B1 by western blotting in the appropriate cell lysate or extract.
Comment

Gene Name: RRM2B

Restrictions For Research Use only

Handling

Product Details anti-RRM2B Antibody Target Details RRM2B Application Details Images back to top
Format Liquid
Concentration 0.82 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

Images

Product Details anti-RRM2B Antibody Target Details RRM2B Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-RRM2B antibody (Ribonucleotide Reductase M2 B (TP53 Inducible)) (N-Term) (ABIN964915) Western blot using affinity purified anti-RRM2B antibody shows detection of recombina...