anti-Human RRM2B antibody for Western Blotting

Recommended RRM2B Antibody (supplied by: Log in to see )

Antigen
Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) Antibodies
  • p53r2
  • RRM2B
  • MTDPS8A
  • MTDPS8B
  • P53R2
  • p53R2
  • ribonucleotide reductase M2 B (TP53 inducible)
  • rrm2b
  • RRM2B
  • Rrm2b
Epitope
AA 1-351
14
12
12
10
9
8
6
3
2
2
2
2
1
1
1
Reactivity
Human
88
44
41
4
3
3
2
2
2
2
1
1
1
1
1
1
Host
Mouse
83
5
Clonality
Monoclonal
Conjugate
This RRM2B antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
ELISA, ELISA (Detection), Western Blotting (WB)
67
38
17
14
13
8
4
2
2
1
1
Supplier
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Catalog No. ABIN1999939
$ 218.90
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.3140206 ABIN3042928 WB Rabbit IgG AA 318-332, C-Term Log in to see Polyclonal
3.3140206 ABIN3186287 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal
3.3140206 ABIN2782441 WB Rabbit Middle Region Log in to see Polyclonal
3.3140206 ABIN1537814 WB Rabbit Ig AA 128-157, Center Log in to see Polyclonal
3.3140206 ABIN964915 IP IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
3.3140206 ABIN4343021 IHC IHC (p) WB Rabbit IgG Center Log in to see Polyclonal
3.3140206 ABIN964916 IP IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
3.3140206 ABIN4343019 ELISA ICC IF IHC IHC (p) WB Rabbit N-Term Log in to see Polyclonal
3.3140206 ABIN1999943 IHC (p) ELISA WB Rabbit IgG AA 1-351 Log in to see Polyclonal
3.3140206 ABIN4905068 WB Rabbit IgG Log in to see Polyclonal
3.3140206 ABIN2649403 WB Rabbit IgG AA 1-351 Log in to see 074
3.3140206 ABIN1031501 IHC ELISA WB Rabbit N-Term Log in to see Polyclonal
3.3140206 ABIN3032475 WB Rabbit IgG C-Term Log in to see Polyclonal
3.3140206 ABIN1852784 ELISA WB Rabbit Internal Region Log in to see Polyclonal
1 ABIN5551853 EIA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN565482 IF ELISA WB Mouse IgG2b kappa AA 3-84, partial Log in to see 6C1 2
1 ABIN5555093 EIA IHC (p) WB Rabbit IgG AA 2-17, N-Term Log in to see Polyclonal
1 ABIN1104077 IF IP IHC ELISA WB Rabbit IgG N-Term Log in to see Polyclonal
1 ABIN303239 IHC (p) WB Rabbit AA 2-17 Log in to see Polyclonal
1 ABIN214469 IHC (p) WB Rabbit AA 2-17 Log in to see Polyclonal

Top referenced anti-RRM2B antibody for Western Blotting

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Application / Reactivity Human
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Enzyme Immunoassay (EIA) 2 Antibodies
Immunocytochemistry (ICC) 2 Antibodies
Immunofluorescence (IF) 8 Antibodies
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Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 13 Antibodies
Immunohistochemistry (IHC) 17 Antibodies
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Immunoprecipitation (IP) 4 Antibodies
Western Blotting (WB) 68 Antibodies

General

Antigen Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) Antibodies
Epitope AA 1-351
(14), (12), (12), (10), (9), (8), (6), (3), (2), (2), (2), (2), (1), (1), (1)
Reactivity Human
(88), (44), (41), (4), (3), (3), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Host Mouse
(83), (5)
Clonality (Clone)
Monoclonal   ( )
Conjugate This RRM2B antibody is un-conjugated
(3), (3), (3), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, ELISA (Detection), Western Blotting (WB)
(67), (38), (17), (14), (13), (8), (4), (2), (2), (1), (1)
Pubmed 1 reference available
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Product Details anti-RRM2B Antibody

Target Details RRM2B Application Details Handling ProductDetails: References for anti-RRM2B antibody (ABIN1999939) Images
Specificity Human RRM2B / P53R2
No cross-reactivity in ELISA with
E.coli cell lysate
No Cross-Reactivity Escherichia coli (E. coli)
Characteristics This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human RRM2B / P53R2 (rh RRM2B / P53R2, ABIN2005498 Q7LG56-1, Met 1-Phe 351,. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
Purification purified by Protein A affinity chromatography
Sterility 0.2 μm filtered
Immunogen Recombinant Human RRM2B / P53R2 protein (ABIN2005498)
Clone 04
Isotype IgG1

Target Details RRM2B

Product Details anti-RRM2B Antibody Application Details Handling ProductDetails: References for anti-RRM2B antibody (ABIN1999939) Images back to top
Antigen
Alternative Name RRM2B (RRM2B Antibody Abstract)
Background Ribonucleoside reductase subunit M2B, also known as RRM2B or p53R2, is an enzyme belonging to the iron-dependent ribonucleotide reductase (RNR) enzyme family which is essential for DNA synthesis. Ribonucleotide reductase (RNR) is an enzyme that catalyzes the formation of deoxyribonucleotides from ribonucleotides and plays a critical role in regulating the total rate of DNA synthesis so that DNA to cell mass is maintained at a constant ratio during cell division and DNA repair. RRM2B is a phosphorylated protein. It is hypothesized that RRM2B activity can be regulated at the posttranslational level in response to DNA damage. RRM2B has previously been shown to be essential for the maintenance of mtDNA copy number and its candidacy for tumor suppression has been evaluated in several mutational analyses of different cancer types. However, the contribution of RRM2B to the DNA damage response has been questioned because its transcriptional induction upon DNA damage is not rapid enough for prompt DNA repair. Instead, ATM-mediated phosphorylation has been suggested to regulate the DNA repair activity of RRM2B posttranslationally. In addition, a defect in RRM2B can induce a mild muscle disease of adult onset through disturbance of mitochondrial homeostasis but that this defect does not appear to be oncogenic.,
UniProt Q7LG56-1
Pathways p53 Signaling, Negative Regulation of intrinsic apoptotic Signaling

Application Details

Product Details anti-RRM2B Antibody Target Details RRM2B Handling ProductDetails: References for anti-RRM2B antibody (ABIN1999939) Images back to top
Application Notes WB: 20-30 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Human RRM2B. The detection limit for Human RRM2B is approximately 0.039 ng/well.
ELISA(Det): 0.1-1 μg/mL
Restrictions For Research Use only

Handling

Product Details anti-RRM2B Antibody Target Details RRM2B Application Details ProductDetails: References for anti-RRM2B antibody (ABIN1999939) Images back to top
Format Liquid
Buffer 0.2 μm filtered solution in PBS with 5 % trehalose
Preservative Without preservative
Handling Advice Preservative-Free. Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment This antibody can be stored at 2°C-8°C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20°C to -80°C. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Expiry Date 12 months

ProductDetails: References for anti-RRM2B antibody (ABIN1999939)

Product Details anti-RRM2B Antibody Target Details RRM2B Application Details Handling Images back to top
Background publications

Bourdon, Minai, Serre, Jais, Sarzi, Aubert, Chrétien, de Lonlay, Paquis-Flucklinger, Arakawa, Nakamura, Munnich, Rötig: "Mutation of RRM2B, encoding p53-controlled ribonucleotide reductase (p53R2), causes severe mitochondrial DNA depletion." in: Nature genetics, Vol. 39, Issue 6, pp. 776-80, 2007

Images

Product Details anti-RRM2B Antibody Target Details RRM2B Application Details Handling ProductDetails: References for anti-RRM2B antibody (ABIN1999939) back to top
Supplier Images
Western Blotting (WB) image for anti-RRM2B antibody (Ribonucleotide Reductase M2 B (TP53 Inducible)) (AA 1-351) (ABIN1999939) anti-Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) (AA 1-351) antibody