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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|ELISA||8 Antibodies||8 Antibodies|
|Enzyme Immunoassay (EIA)||1 Antibodies||1 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies||1 Antibodies|
|Immunofluorescence (IF)||2 Antibodies||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||11 Antibodies||11 Antibodies|
|Immunohistochemistry (IHC)||6 Antibodies||8 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||6 Antibodies||6 Antibodies|
|Western Blotting (WB)||24 Antibodies||24 Antibodies|
|Antigen||Ribonucleotide Reductase M2 B (TP53 Inducible) (RRM2B) Antibodies|
|Epitope||AA 2-17, N-Term Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This RRM2B antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
|4 references available|
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Product Details anti-RRM2B AntibodyTarget Details RRM2B Application Details Handling References for anti-RRM2B Antibody (ABIN500437) Images
|Specificity||This antibody detects p53R2 at N-term.|
|Purification||Peptide Affinity Chromatography|
|Immunogen||p53R2 antibody was raised against a synthetic peptide corresponding to amino acids 2 to 17 of human p53R2.|
Target Details RRM2BProduct Details anti-RRM2B Antibody Application Details Handling References for anti-RRM2B Antibody (ABIN500437) Images back to top
|Alternative Name||RRM2B / P53R2 (RRM2B Antibody Abstract)|
|Background||The p53 tumor-suppresspr gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2 , Chk2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase (1,7). p53R2 is induced by the reagents, ultraviolet and g-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression (8). p53R2 is an important target of p53 for tumour suppression.Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein|
Application DetailsProduct Details anti-RRM2B Antibody Target Details RRM2B Handling References for anti-RRM2B Antibody (ABIN500437) Images back to top
ELISA. Western blot: 0.5-1 μg/mL (A 39 kDa band can be detected). Immunohistochemistry on Paraffin Sections. Positive Controls: A431 Cell Lysate, 3T3 Whole Cell Lysate, Human Lung Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-RRM2B Antibody Target Details RRM2B Application Details References for anti-RRM2B Antibody (ABIN500437) Images back to top
|Buffer||PBS containing 0.02 % Sodium Azide|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-RRM2B Antibody (ABIN500437)Product Details anti-RRM2B Antibody Target Details RRM2B Application Details Handling Images back to top
Oda, Arakawa, Tanaka et al.: "p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53." in: Cell, Vol. 102, Issue 6, pp. 849-62, 2000 (PubMed).
Oda, Ohki, Murasawa et al.: "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis." in: Science (New York, N.Y.), Vol. 288, Issue 5468, pp. 1053-8, 2000 (PubMed).
Tanaka, Arakawa, Yamaguchi et al.: "A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage." in: Nature, Vol. 404, Issue 6773, pp. 42-9, 2000 (PubMed).
Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).
ImagesProduct Details anti-RRM2B Antibody Target Details RRM2B Application Details Handling References for anti-RRM2B Antibody (ABIN500437) back to top