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anti-Human SCYL1 antibody for Western Blotting

Recommended SCYL1 Antibody (supplied by: Log in to see )

Antigen
SCY1-Like 1 (S. Cerevisiae) (SCYL1) Antibodies
  • 2810011O19Rik
  • C85140
  • DDBDRAFT_0189357
  • DDBDRAFT_0220502
  • DDB_0189357
  • DDB_0220502
  • GKLP
  • mdf
  • mfd
  • NKTL
  • Ntkl
  • NTKL
  • P105
  • p105
  • TAPK
  • TEIF
  • TRAP
Epitope
AA 373-472
18
9
3
2
1
1
1
Reactivity
Human
38
1
Host
Mouse
29
9
Clonality
Monoclonal
Conjugate
This SCYL1 antibody is un-conjugated
3
3
3
3
3
3
Application
Western Blotting (WB)
34
23
19
5
3
2
2
1
Supplier
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Catalog No. ABIN393395
$ 450.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.13341247 ABIN528172 ELISA WB Mouse IgG2a kappa AA 373-472, partial Log in to see 2E5 1
0.13341247 ABIN652299 IHC (p) WB Rabbit Ig AA 156-185, N-Term Log in to see Polyclonal
0.13341247 ABIN2788975 WB Rabbit C-Term Log in to see Polyclonal
0.13341247 ABIN2502107 IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.13341247 ABIN926830 WB Rabbit C-Term Log in to see Polyclonal
0.0011728023 ABIN798702 WB Rabbit C-Term Log in to see Polyclonal
0.0011728023 ABIN1933202 IHC ELISA WB PE Rabbit IgG N-Term Log in to see Polyclonal
0.0011728023 ABIN1933198 IHC ELISA WB Alkaline Phosphatase (AP) Rabbit IgG N-Term Log in to see Polyclonal
0.0011728023 ABIN1933200 IHC ELISA WB FITC Rabbit IgG N-Term Log in to see Polyclonal
0.0011728023 ABIN1933199 IHC ELISA WB APC Rabbit IgG N-Term Log in to see Polyclonal
0.0011728023 ABIN1933201 IHC ELISA WB Biotin Rabbit IgG N-Term Log in to see Polyclonal
0.0011728023 ABIN1933203 IHC ELISA WB HRP Rabbit IgG N-Term Log in to see Polyclonal
0.0011728023 ABIN442998 ICC IF WB Rabbit IgG Log in to see Polyclonal
0.0011728023 ABIN1933193 ELISA WB Biotin Mouse IgG2a AA 373-472 Log in to see
0.0011728023 ABIN1933192 ELISA WB Alkaline Phosphatase (AP) Mouse IgG2a AA 373-472 Log in to see
0.0011728023 ABIN1933194 ELISA WB FITC Mouse IgG2a AA 373-472 Log in to see
0.0011728023 ABIN1933191 ELISA WB APC Mouse IgG2a AA 373-472 Log in to see
0.0011728023 ABIN1933195 ELISA WB PE Mouse IgG2a AA 373-472 Log in to see
0.0011728023 ABIN1933196 ELISA WB HRP Mouse IgG2a AA 373-472 Log in to see
0.0011728023 ABIN1933197 IP WB Rabbit IgG AA 758-808 Log in to see Polyclonal

Top referenced anti-SCYL1 antibody for Western Blotting

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Application / Reactivity Human
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Immunocytochemistry (ICC) 3 Antibodies
Immunofluorescence (IF) 5 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 2 Antibodies
Immunoprecipitation (IP) 2 Antibodies
Western Blotting (WB) 35 Antibodies

General

Antigen SCY1-Like 1 (S. Cerevisiae) (SCYL1) Antibodies
Epitope AA 373-472
(18), (9), (3), (2), (1), (1), (1)
Reactivity Human
(38), (1)
Host Mouse
(29), (9)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SCYL1 antibody is un-conjugated
(3), (3), (3), (3), (3), (3)
Application Western Blotting (WB)
(34), (23), (19), (5), (3), (2), (2), (1)
Pubmed 5 references available
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Product Details anti-SCYL1 Antibody

Target Details SCYL1 Application Details Handling References for anti-SCYL1 Antibody (ABIN393395) Images
Specificity SCYL1 (AAH09967, 373 a.a. ~ 472 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
Purification This antibody is purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen SCYL1 (AAH09967, 373 a.a. ~ 472 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 K
Clone 2E5
Isotype IgG2a kappa

Target Details SCYL1

Product Details anti-SCYL1 Antibody Application Details Handling References for anti-SCYL1 Antibody (ABIN393395) Images back to top
Antigen
Alternative Name SCYL1 (SCYL1 Antibody Abstract)
Background Synonyms: Serine protease inhibitor J6, 47 kDa heat shock protein, Collagen-binding protein, Colligin, Serpin H1,Hsp47, Cbp1, Serpinh1
Molecular Weight 89631 DA
Gene ID 12406
Research Area Phospho-specific antibodies, Protein Modifications, Transcription Factors, Cell Structure
Pathways p53 Signaling

Application Details

Product Details anti-SCYL1 Antibody Target Details SCYL1 Handling References for anti-SCYL1 Antibody (ABIN393395) Images back to top
Application Notes Western blot = 1:500-1000
Comment

Background: This gene encodes a transcriptional regulator belonging to the SCY1-like family of kinase-like proteins. The protein has a divergent N-terminal kinase domain that is thought to be catalytically inactive, and can bind specific DNA sequences through its C-terminal domain. It activates transcription of the telomerase reverse transcriptase and DNA polymerase beta genes. The protein has been localized to the nucleus, and also to the cytoplasm and centrosomes during mitosis. Multiple transcript variants encoding different isoforms have been found for this gene.

Restrictions For Research Use only

Handling

Product Details anti-SCYL1 Antibody Target Details SCYL1 Application Details References for anti-SCYL1 Antibody (ABIN393395) Images back to top
Buffer PBS with 0.09% (w/v) sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months

References for anti-SCYL1 Antibody (ABIN393395)

Product Details anti-SCYL1 Antibody Target Details SCYL1 Application Details Handling Images back to top
Background publications

Yan, Zhang, Di, Shi, Rao, Huo: "A newly identified Pirh2 substrate SCYL1-BP1 can bind to MDM2 and accelerate MDM2 self-ubiquitination." in: FEBS letters, Vol. 584, Issue 15, pp. 3275-8, 2010

Gong, Sun, McNutt, Sun, Hou, Liu, Shen, Ling, Chi, Zhang: "Localization of TEIF in the centrosome and its functional association with centrosome amplification in DNA damage, telomere dysfunction and human cancers." in: Oncogene, Vol. 28, Issue 12, pp. 1549-60, 2009

Burman, Bourbonniere, Philie, Stroh, Dejgaard, Presley, McPherson: "Scyl1, mutated in a recessive form of spinocerebellar neurodegeneration, regulates COPI-mediated retrograde traffic." in: The Journal of biological chemistry, Vol. 283, Issue 33, pp. 22774-86, 2008

Barbe, Lundberg, Oksvold, Stenius, Lewin, Björling, Asplund, Pontén, Brismar, Uhlén, Andersson-Svahn: "Toward a confocal subcellular atlas of the human proteome." in: Molecular & cellular proteomics : MCP, Vol. 7, Issue 3, pp. 499-508, 2008

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Images

Product Details anti-SCYL1 Antibody Target Details SCYL1 Application Details Handling References for anti-SCYL1 Antibody (ABIN393395) back to top
Supplier Images
Western Blotting (WB) image for anti-SCYL1 antibody (SCY1-Like 1 (S. Cerevisiae)) (AA 373-472) (ABIN393395) SCYL1 monoclonal antibody (M02), clone 2E5. Western Blot analysis of SCYL1 expression...