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anti-Human SERPINE1 antibody for Functional Studies

Recommended SERPINE1 Antibody (supplied by: Log in to see )

Antigen
serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies
  • B230326M24Rik
  • HsT1201
  • PAI
  • PAI-1
  • PAI-2
  • Pai1
  • PAI1
  • PAI1A
  • Pai1aa
  • PAI2
  • PI-7
  • PI7
  • Planh
  • Planh1
  • PLANH1
  • PLANH2
  • PN-1
  • RATPAI1A
  • SERPINE1
  • si:ch211-138a11.1
  • Spi4
Reactivity
Human, Mouse (Murine), Rat (Rattus)
313
99
92
31
11
9
3
2
2
1
1
1
1
Host
Mouse
223
112
49
28
2
1
Clonality
Monoclonal
Conjugate
This SERPINE1 antibody is un-conjugated
41
28
16
2
2
2
2
2
2
2
2
2
2
2
2
2
1
Application
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
278
200
87
48
43
27
23
21
18
16
9
6
6
6
5
5
4
4
4
4
3
1
1
1
1
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Catalog No. ABIN112269
$ 2,062.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008770663 ABIN1108976 Func EIA WB Mouse IgG1 Log in to see MA-33H1F7 5
0.0008770663 ABIN1108974 Func EIA Mouse IgG1 Log in to see MA-56A7C10 2
0.0008770663 ABIN1108977 Func EIA IHC (fro) WB Mouse IgG1 Log in to see MA-33H1F7 3
0.0008770663 ABIN1108975 Func EIA Mouse IgG1 Log in to see MA-55F4C12 3

Top referenced anti-SERPINE1 antibody for Functional Studies

Similar anti-SERPINE1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Antibody (Inhibition) 3 Antibodies 3 Antibodies
ELISA 32 Antibodies 24 Antibodies
ELISA (Capture) 3 Antibodies 3 Antibodies
ELISA (Detection) 14 Antibodies 12 Antibodies
Enzyme Immunoassay (EIA) 5 Antibodies 5 Antibodies
Functional Studies (Func) 5 Antibodies
Immunocytochemistry (ICC) 1 Antibodies
Immunofluorescence (IF) 2 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 22 Antibodies
Immunohistochemistry (IHC) 19 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 9 Antibodies
Immunoprecipitation (IP) 6 Antibodies
Western Blotting (WB) 58 Antibodies

General

Antigen serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(313), (99), (92), (31), (11), (9), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(223), (112), (49), (28), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SERPINE1 antibody is un-conjugated
(41), (28), (16), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1)
Application Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
(278), (200), (87), (48), (43), (27), (23), (21), (18), (16), (9), (6), (6), (6), (5), (5), (4), (4), (4), (4), (3), (1), (1), (1), (1)
Pubmed 11 references available
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Product Details anti-SERPINE1 Antibody

Target Details SERPINE1 Application Details Handling References for anti-SERPINE1 Antibody (ABIN112269) Images
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse and Rat.
Purification Protein A Affinity Chromatography of Hybridoma Cell Culture Supernatant
Purity > 98 % (determined by SDS PAGE).
Immunogen Recombinant Human Plasminogen Activator protein 1 (non-glycosylated).
Clone 33H1F7
Isotype IgG1

Target Details SERPINE1

Product Details anti-SERPINE1 Antibody Application Details Handling References for anti-SERPINE1 Antibody (ABIN112269) Images back to top
Antigen
Alternative Name SERPINE1 / PAI1 (SERPINE1 Antibody Abstract)
Background PAI1 (plasminogen activator inhibitor 1) is originally cloned from human endothelial cell (Pannekoek 1986, Ginsburg 1986) and rat hepatoma cell 3 cDNA libraries. As a member of the serpin family of serine protease inhibitors, PAI1 inhibits both tissue type plasminogen activator (tPA) and urokinase type plasminogen activator (uPA). High PAI1 levels are associated with an increased risk of thromboembolic disease while PAI1 deficiency may represent an inherited autosomal recessive bleeding disorder.Synonyms: Endothelial plasminogen activator inhibitor, PAI, PAI-1, PLANH1, Plasminogen Activator Inhibitor 1, Serpin E1
NCBI Accession NM_000602
UniProt P05121
Pathways p53 Signaling

Application Details

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Handling References for anti-SERPINE1 Antibody (ABIN112269) Images back to top
Application Notes ELISA. Inhibition studies.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details References for anti-SERPINE1 Antibody (ABIN112269) Images back to top
Concentration 5.29 mg/mL
Buffer 0.05 M Sodium Phosphate, 0.1 M Sodium Chloride, pH 7.4
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-SERPINE1 Antibody (ABIN112269)

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling Images back to top
Reference:

Smolarczyk, Gils, Boncela, Declerck, Cierniewski: "Function-stabilizing mechanism of plasminogen activator inhibitor type 1 induced upon binding to alpha1-acid glycoprotein." in: Biochemistry, Vol. 44, Issue 37, pp. 12384-90, 2005

Ngo, Hoylaerts, Knockaert, Brouwers, Declerck: "Identification of a target site in plasminogen activator inhibitor-1 that allows neutralization of its inhibitor properties concomitant with an allosteric up-regulation of its antiadhesive properties." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 26243-8, 2001

Bijnens, Gils, Knockaert, Stassen, Declerck: "Importance of the hinge region between alpha-helix F and the main part of serpins, based upon identification of the epitope of plasminogen activator inhibitor type 1 neutralizing antibodies." in: The Journal of biological chemistry, Vol. 275, Issue 9, pp. 6375-80, 2000

Verhamme, Kvassman, Day, Debrock, Vleugels, Declerck, Shore: "Accelerated conversion of human plasminogen activator inhibitor-1 to its latent form by antibody binding." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17511-7, 1999

Background publications

Naessens, Gils, Compernolle, Declerck: "Elucidation of the epitope of a latency-inducing antibody: identification of a new molecular target for PAI-1 inhibition." in: Thrombosis and haemostasis, Vol. 90, Issue 1, pp. 52-8, 2003

Montes, Declerck, Calvo, Montes, Hermida, Muñoz, Rocha: "Prevention of renal fibrin deposition in endotoxin-induced DIC through inhibition of PAI-1." in: Thrombosis and haemostasis, Vol. 84, Issue 1, pp. 65-70, 2001

Kornberg: "Remembering our teachers." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 3-11, 2001

Yanai, Saito, Kakinuma, Kon, Hirota, Taniguchi-Yanai, Nishijo, Shigematsu, Horiguchi, Kasuya, Sugiyama, Yagami, Murakami, Fukamizu: "Renin-dependent cardiovascular functions and renin-independent blood-brain barrier functions revealed by renin-deficient mice." in: The Journal of biological chemistry, Vol. 275, Issue 1, pp. 5-8, 2000

Tabor: "Prions of mammals and fungi minireview series." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 1, 1999

Debrock, Declerck: "Identification of a functional epitope in plasminogen activator inhibitor-1, not localized in the reactive center loop." in: Thrombosis and haemostasis, Vol. 79, Issue 3, pp. 597-601, 1998

Debrock, Declerck: "Neutralization of plasminogen activator inhibitor-1 inhibitory properties: identification of two different mechanisms." in: Biochimica et biophysica acta, Vol. 1337, Issue 2, pp. 257-66, 1997