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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Blocking Antibody (Inhibition)||3 Antibodies||3 Antibodies|
|ELISA||32 Antibodies||24 Antibodies|
|ELISA (Capture)||3 Antibodies||3 Antibodies|
|ELISA (Detection)||14 Antibodies||12 Antibodies|
|Enzyme Immunoassay (EIA)||4 Antibodies|
|Functional Studies (Func)||4 Antibodies|
|Immunoassay (IA)||1 Antibodies|
|Immunocytochemistry (ICC)||17 Antibodies|
|Immunofluorescence (IF)||18 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||24 Antibodies|
|Immunohistochemistry (IHC)||35 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||18 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||25 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||73 Antibodies|
|Antigen||serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies|
|Epitope||AA 300-400, Internal Region Alternatives|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SERPINE1 antibody is un-conjugated Alternatives|
Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (IHC), Western Blotting (WB)
|7 references available|
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Product Details anti-SERPINE1 AntibodyTarget Details SERPINE1 Application Details Handling References for anti-SERPINE1 Antibody (ABIN446967) Images
|Specificity||PAI-1 (N315) pAb detects endogenous levels of PAI-1 protein.|
|Purification||Immunogen affinity purified|
|Immunogen||A synthetic peptide made to an internal portion of the human PAI1/Serpine 1 protein (between residues 300-400) [UniProt P05121]|
Target Details SERPINE1Product Details anti-SERPINE1 Antibody Application Details Handling References for anti-SERPINE1 Antibody (ABIN446967) Images back to top
|Alternative Name||Serpin E1/PAI-1 (SERPINE1 Antibody Abstract)|
|Background||Gene Symbol: SERPINE1|
|Molecular Weight||Theoretical MW: 45 kDa|
|Gene ID||5054, 18787, 24617|
Application DetailsProduct Details anti-SERPINE1 Antibody Target Details SERPINE1 Handling References for anti-SERPINE1 Antibody (ABIN446967) Images back to top
|Application Notes||Western Blot 1.0 μg/mL, Immunohistochemistry 1:50 - 1:100, Immunocytochemistry/Immunofluorescence 1:50 - 1:100, Immunohistochemistry-Paraffin 1:50 - 1:100, Immunohistochemistry-Frozen 1:50 - 1:100|
The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.
Western blot Protocol specific for PAI1/Serpine1 antibody Western Blot Protocol
1. Perform SDS-PAGE on samples to be analyzed, loading 40 µg of total protein per lane.
. Transfer proteins to membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
. Stain according to standard Ponceau S procedure (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
. Rinse the blot.
. Block the membrane using standard blocking buffer for at least 1 hour.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Dilute primary antibody in blocking buffer and incubate 1 hour at room temperature.
. Wash the membrane in wash buffer three times for 10 minutes each.
. Apply the diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturers instructions) and incubate 1 hour at room temperature.
. Wash the blot in wash buffer three times for 10 minutes each (this step can be repeated as required to reduce background).
. Apply the detection reagent of choice in accordance with the manufacturers instructions.Note: Tween-20 can be added to the blocking or antibody dilution buffer at a final concentration of 0.05-0.2 %.Immunohistochemistry Protocol specific for PAI1/Serpine1 antibody Immunohistochemistry-Paraffin Embedded SectionsAntigen Unmasking:Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.
0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes. Staining:
. Wash sections in deionized water three times for 5 minutes each.
. Wash sections in wash buffer for 5 minutes.
. Block each section with 100-400 µL blocking solution for 1 hour at room temperature.
. Remove blocking solution and add 100-400 µL diluted primary antibody. Incubate overnight at 4 C.
. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
. Add 100-400 µL biotinylated diluted secondary antibody. Incubate 30 minutes at room temperature.
. Remove secondary antibody solution and wash sections three times with wash buffer for 5 minutes each.
. Add 100-400 µL Streptavidin-HRP reagent to each section and incubate for 30 minutes at room temperature.
. Wash sections three times in wash buffer for 5 minutes each.
. Add 100-400 µL DAB substrate to each section and monitor staining closely.
. As soon as the sections develop, immerse slides in deionized water.
. Counterstain sections in hematoxylin.
. Wash sections in deionized water two times for 5 minutes each.
. Dehydrate sections.
. Mount coverslips.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details References for anti-SERPINE1 Antibody (ABIN446967) Images back to top
Buffer contains: 0.02 % Sodium Azide
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Handling Advice||Avoid freeze-thaw cycles|
|Storage||4 °C,-20 °C|
|Storage Comment||Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.|
References for anti-SERPINE1 Antibody (ABIN446967)Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling Images back to top
|Product cited in:||
Sitaram, Mallikarjuna, Landström, Ljungberg: "Transforming growth factor-β promotes aggressiveness and invasion of clear cell renal cell carcinoma." in: Oncotarget, Vol. 7, Issue 24, pp. 35917-35931, 2016 (Sample species: Human). Further details: Western Blotting
Liao, Wang, Guo, Lin, Chang, Juo: "Let-7g improves multiple endothelial functions through targeting transforming growth factor-beta and SIRT-1 signaling." in: Journal of the American College of Cardiology, Vol. 63, Issue 16, pp. 1685-94, 2014 (Sample species: Mouse (Murine)). Further details: Immunocytochemistry,Immunofluorescence,Immunohistochemistry (Frozen Sections)
Longchamp, Alonso, Dubuis, Allagnat, Berard, Meda, Saucy, Corpataux, Déglise, Haefliger: "The use of external mesh reinforcement to reduce intimal hyperplasia and preserve the structure of human saphenous veins." in: Biomaterials, Vol. 35, Issue 9, pp. 2588-99, 2014 (Sample species: Human). Further details: Western Blotting
Lee, Oh, Park, Na, Gil, Yang, Lee, Hong: "Urokinase, urokinase receptor, and plasminogen activator inhibitor-1 expression on podocytes in immunoglobulin A glomerulonephritis." in: The Korean journal of internal medicine, Vol. 29, Issue 2, pp. 176-82, 2014 (Sample species: Human).
Berard, Déglise, Alonso, Saucy, Meda, Bordenave, Corpataux, Haefliger: "Role of hemodynamic forces in the ex vivo arterialization of human saphenous veins." in: Journal of vascular surgery, Vol. 57, Issue 5, pp. 1371-82, 2013 (Sample species: Human). Further details: Western Blotting
Dubuis, May, Alonso, Luca, Mylonaki, Meda, Delie, Jordan, Déglise, Corpataux, Saucy, Haefliger: "Atorvastatin-loaded hydrogel affects the smooth muscle cells of human veins." in: The Journal of pharmacology and experimental therapeutics, Vol. 347, Issue 3, pp. 574-81, 2013 (Sample species: Human). Further details: Immunocytochemistry,Western Blotting,Immunofluorescence
Cao, Szabolcs, Dutta, Yaqoob, Jagavelu, Wang, Leof, Urrutia, Shah, Mukhopadhyay: "Neuropilin-1 mediates divergent R-Smad signaling and the myofibroblast phenotype." in: The Journal of biological chemistry, Vol. 285, Issue 41, pp. 31840-8, 2010 (Sample species: Human). Further details: Western Blotting
ImagesProduct Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling References for anti-SERPINE1 Antibody (ABIN446967) back to top