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|Application / Reactivity||Rat (Rattus)||Mouse (Murine)|
|Blocking Antibody (Inhibition)||3 Antibodies||3 Antibodies|
|ELISA||32 Antibodies||24 Antibodies|
|ELISA (Capture)||3 Antibodies||3 Antibodies|
|ELISA (Detection)||14 Antibodies||12 Antibodies|
|Enzyme Immunoassay (EIA)||5 Antibodies||5 Antibodies|
|Functional Studies (Func)||5 Antibodies|
|Immunocytochemistry (ICC)||1 Antibodies|
|Immunofluorescence (IF)||2 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||22 Antibodies|
|Immunohistochemistry (IHC)||19 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||3 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||9 Antibodies|
|Immunoprecipitation (IP)||6 Antibodies|
|Western Blotting (WB)||57 Antibodies|
|Antigen||serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies|
|Reactivity||Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This SERPINE1 antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Sections) (IHC (fro)), Western Blotting (WB)
|11 references available|
|Supplier||Log in to see|
Product Details anti-SERPINE1 AntibodyTarget Details SERPINE1 Application Details Handling References for anti-SERPINE1 Antibody (ABIN112269) Images
|Cross-Reactivity (Details)||Species reactivity (tested):Human, Mouse and Rat.|
|Purification||Protein A Affinity Chromatography of Hybridoma Cell Culture Supernatant|
|Purity||> 98 % (determined by SDS PAGE).|
|Immunogen||Recombinant Human Plasminogen Activator protein 1 (non-glycosylated).|
Target Details SERPINE1Product Details anti-SERPINE1 Antibody Application Details Handling References for anti-SERPINE1 Antibody (ABIN112269) Images back to top
|Alternative Name||SERPINE1 / PAI1 (SERPINE1 Antibody Abstract)|
|Background||PAI1 (plasminogen activator inhibitor 1) is originally cloned from human endothelial cell (Pannekoek 1986, Ginsburg 1986) and rat hepatoma cell 3 cDNA libraries. As a member of the serpin family of serine protease inhibitors, PAI1 inhibits both tissue type plasminogen activator (tPA) and urokinase type plasminogen activator (uPA). High PAI1 levels are associated with an increased risk of thromboembolic disease while PAI1 deficiency may represent an inherited autosomal recessive bleeding disorder.Synonyms: Endothelial plasminogen activator inhibitor, PAI, PAI-1, PLANH1, Plasminogen Activator Inhibitor 1, Serpin E1|
Application DetailsProduct Details anti-SERPINE1 Antibody Target Details SERPINE1 Handling References for anti-SERPINE1 Antibody (ABIN112269) Images back to top
ELISA. Inhibition studies.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details References for anti-SERPINE1 Antibody (ABIN112269) Images back to top
|Buffer||0.05 M Sodium Phosphate, 0.1 M Sodium Chloride, pH 7.4|
|Handling Advice||Avoid repeated freezing and thawing.|
|Storage||4 °C/-20 °C|
|Storage Comment||Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.|
References for anti-SERPINE1 Antibody (ABIN112269)Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling Images back to top
Naessens, Gils, Compernolle et al.: "Elucidation of the epitope of a latency-inducing antibody: identification of a new molecular target for PAI-1 inhibition." in: Thrombosis and haemostasis, Vol. 90, Issue 1, pp. 52-8, 2003 (PubMed).
Kornberg: "Remembering our teachers." in: The Journal of biological chemistry, Vol. 276, Issue 1, pp. 3-11, 2001 (PubMed).
Montes, Declerck, Calvo et al.: "Prevention of renal fibrin deposition in endotoxin-induced DIC through inhibition of PAI-1." in: Thrombosis and haemostasis, Vol. 84, Issue 1, pp. 65-70, 2001 (PubMed).
Yanai, Saito, Kakinuma et al.: "Renin-dependent cardiovascular functions and renin-independent blood-brain barrier functions revealed by renin-deficient mice." in: The Journal of biological chemistry, Vol. 275, Issue 1, pp. 5-8, 2000 (PubMed).
Tabor: "Prions of mammals and fungi minireview series." in: The Journal of biological chemistry, Vol. 274, Issue 1, pp. 1, 1999 (PubMed).
Debrock, Declerck: "Identification of a functional epitope in plasminogen activator inhibitor-1, not localized in the reactive center loop." in: Thrombosis and haemostasis, Vol. 79, Issue 3, pp. 597-601, 1998 (PubMed).
Debrock, Declerck: "Neutralization of plasminogen activator inhibitor-1 inhibitory properties: identification of two different mechanisms." in: Biochimica et biophysica acta, Vol. 1337, Issue 2, pp. 257-66, 1997 (PubMed).
Smolarczyk, Gils, Boncela et al.: "Function-stabilizing mechanism of plasminogen activator inhibitor type 1 induced upon binding to alpha1-acid glycoprotein." in: Biochemistry, Vol. 44, Issue 37, pp. 12384-90, 2005 (PubMed).
Ngo, Hoylaerts, Knockaert et al.: "Identification of a target site in plasminogen activator inhibitor-1 that allows neutralization of its inhibitor properties concomitant with an allosteric up-regulation of its antiadhesive properties." in: The Journal of biological chemistry, Vol. 276, Issue 28, pp. 26243-8, 2001 (PubMed).
Bijnens, Gils, Knockaert et al.: "Importance of the hinge region between alpha-helix F and the main part of serpins, based upon identification of the epitope of plasminogen activator inhibitor type 1 neutralizing antibodies." in: The Journal of biological chemistry, Vol. 275, Issue 9, pp. 6375-80, 2000 (PubMed).
Verhamme, Kvassman, Day et al.: "Accelerated conversion of human plasminogen activator inhibitor-1 to its latent form by antibody binding." in: The Journal of biological chemistry, Vol. 274, Issue 25, pp. 17511-7, 1999 (PubMed).