anti-Rat (Rattus) SERPINE1 antibody for Enzyme Immunoassay

Recommended SERPINE1 Antibody (supplied by: Log in to see )

Antigen
serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies
  • PAI
  • PAI-1
  • PAI1
  • PLANH1
  • si:ch211-138a11.1
  • SERPINE1
  • Planh1
  • B230326M24Rik
  • PI-7
  • PI7
  • PN-1
  • Spi4
  • HsT1201
  • PAI-2
  • PAI2
  • PLANH2
  • PAI1A
  • Pai1
  • Pai1aa
  • Planh
  • RATPAI1A
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • plasminogen activator inhibitor 1
  • plasminogen activator inhibitor type 1
  • serine (or cysteine) peptidase inhibitor, clade E, member 1
  • serine (or cysteine) peptidase inhibitor, clade E, member 2
  • serpin peptidase inhibitor, clade B (ovalbumin), member 2
  • serpine1
  • SERPINE1
  • CpipJ_CPIJ011718
  • CpipJ_CPIJ016298
  • PAI-1
  • Serpine1
  • Serpine2
  • SERPINB2
Reactivity
Human, Mouse (Murine), Rat (Rattus)
365
134
128
41
18
13
7
6
4
4
3
2
2
2
1
1
Host
Mouse
260
126
55
26
2
1
Clonality
Monoclonal
Conjugate
This SERPINE1 antibody is un-conjugated
44
30
17
5
4
3
3
3
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
Application
Functional Studies (Func), Enzyme Immunoassay (EIA), Western Blotting (WB)
331
216
119
74
44
43
36
27
27
26
20
18
10
6
6
6
4
4
4
4
4
3
1
1
1
1
1
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Catalog No. ABIN1108976
$ 423.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1108973 Func EIA Horse IgG1 Log in to see MA-124K1 1
1 ABIN1108977 Func EIA IHC (fro) WB Mouse IgG1 Log in to see MA-33H1F7 3
1 ABIN1108975 Func EIA Mouse IgG1 Log in to see MA-55F4C12 3

Top referenced anti-SERPINE1 antibody for Enzyme Immunoassay

Similar anti-SERPINE1 Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Blocking Antibody (Inhibition) 3 Antibodies 3 Antibodies
ELISA 35 Antibodies 29 Antibodies
ELISA (Capture) 3 Antibodies 3 Antibodies
ELISA (Detection) 14 Antibodies
Enzyme Immunoassay (EIA) 4 Antibodies
Functional Studies (Func) 4 Antibodies
Immunoassay (IA) 1 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 19 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 1 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 32 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 19 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 27 Antibodies
Immunoprecipitation (IP) 7 Antibodies
Western Blotting (WB) 88 Antibodies

General

Antigen serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) Antibodies
Reactivity Human, Mouse (Murine), Rat (Rattus)
(365), (134), (128), (41), (18), (13), (7), (6), (4), (4), (3), (2), (2), (2), (1), (1)
Host Mouse
(260), (126), (55), (26), (2), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This SERPINE1 antibody is un-conjugated
(44), (30), (17), (5), (4), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application Functional Studies (Func), Enzyme Immunoassay (EIA), Western Blotting (WB)
(331), (216), (119), (74), (44), (43), (36), (27), (27), (26), (20), (18), (10), (6), (6), (6), (4), (4), (4), (4), (4), (3), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-SERPINE1 Antibody

Target Details SERPINE1 Application Details Handling ProductDetails: References for anti-SERPINE1 antibody (ABIN1108976) Images
Specificity This antibody detects PAI-1. The epitope of the antibody does not cover the complete alpha-helix F and turn connecting alpha-helix F and beta-strand s3A, but is restricted to the hinge region between alpha-helix F and the main part of the PAI-1 Molecule. The monoclonal antibody MA-33H1F7 is a 'switching' antibody, capable of inducing a non-inhibitory substrate form of PAI-1. It was shown to inhibit PAI-1 in a dose dependent manner.
Cross-Reactivity (Details) Species reactivity (tested):Human, rat, mouse
Purification Protein G
Clone MA-33H1F7
Isotype IgG1

Target Details SERPINE1

Product Details anti-SERPINE1 Antibody Application Details Handling ProductDetails: References for anti-SERPINE1 antibody (ABIN1108976) Images back to top
Antigen
Alternative Name SERPINE1 / PAI1 (SERPINE1 Antibody Abstract)
Background Plasminogen activator inhibitor type-1 (PAI-1), a member of the serine protease inhibitor (serpin) superfamily, is an important protein in the regulation of fibrinolysis. PAI-1 is unique among the serpins because of its functional and conformational flexibility. PAI-1 is the most important physiological inhibitor of both tissue-type plasminogen activator (t-PA) and urokinase-type plasminogen activator (u-PA). Increased PAI-1 levels are associated with thrombotic events and is an established risk factor for cardiovascular diseases. The active conformation PAI-1 inhibits its target proteinases by the formation of a stable, inactive complex. Although PAI-1 is synthesized as an active molecule, it converts spontaneously to an inactive, latent form that can be partially reactivated by denaturing agents. In addition, a third conformation reacting as a non-inhibitory substrate towards various target proteinases has been identified. The epitope of monoclonal antibody MA-33H1F7 is predominantly composed of three residues (Lys154/Glu130/Arg131), positioned virtually linearly in the three-dimensional structure.Synonyms: Endothelial plasminogen activator inhibitor, PAI, PAI-1, PLANH1, Plasminogen Activator Inhibitor 1, Serpin E1
Gene ID 5054
NCBI Accession NP_000593
UniProt P05121
Pathways p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration

Application Details

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Handling ProductDetails: References for anti-SERPINE1 antibody (ABIN1108976) Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details ProductDetails: References for anti-SERPINE1 antibody (ABIN1108976) Images back to top
Concentration 0.1 mg/mL
Buffer PBS, 0.1 % bovine serum albumin
Storage 4 °C
Storage Comment Store at 2 - 8 °C.

ProductDetails: References for anti-SERPINE1 antibody (ABIN1108976)

Product Details anti-SERPINE1 Antibody Target Details SERPINE1 Application Details Handling Images back to top
Background publications

Komissarov, Andreasen, Declerck, Kamikubo, Zhou, Gruber: "Redirection of the reaction between activated protein C and a serpin to the substrate pathway." in: Thrombosis research, Vol. 122, Issue 3, pp. 397-404, 2008

Rupin, Martin, Vallez, Bonhomme, Verbeuren: "Inactivation of plasminogen activator inhibitor-1 accelerates thrombolysis of a platelet-rich thrombus in rat mesenteric arterioles." in: Thrombosis and haemostasis, Vol. 86, Issue 6, pp. 1528-31, 2002

Bijnens, Gils, Knockaert, Stassen, Declerck: "Importance of the hinge region between alpha-helix F and the main part of serpins, based upon identification of the epitope of plasminogen activator inhibitor type 1 neutralizing antibodies." in: The Journal of biological chemistry, Vol. 275, Issue 9, pp. 6375-80, 2000

Berry, Lunven, Lechaire, Girardot, OConnor: "Antithrombotic activity of a monoclonal antibody inducing the substrate form of plasminogen activator inhibitor type 1 in rat models of venous and arterial thrombosis." in: British journal of pharmacology, Vol. 125, Issue 1, pp. 29-34, 1998

Debrock, Declerck: "Neutralization of plasminogen activator inhibitor-1 inhibitory properties: identification of two different mechanisms." in: Biochimica et biophysica acta, Vol. 1337, Issue 2, pp. 257-66, 1997