Human SERPINE1 ELISA Kit

Recommended SERPINE1 Product (supplied by: Log in to see )

Antigen
serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kits
  • PAI
  • PAI-1
  • PAI1
  • PLANH1
  • si:ch211-138a11.1
  • SERPINE1
  • Planh1
  • B230326M24Rik
  • PI-7
  • PI7
  • PN-1
  • Spi4
  • HsT1201
  • PAI-2
  • PAI2
  • PLANH2
  • PAI1A
  • Pai1
  • Pai1aa
  • Planh
  • RATPAI1A
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • plasminogen activator inhibitor 1
  • plasminogen activator inhibitor type 1
  • serine (or cysteine) peptidase inhibitor, clade E, member 1
  • serine (or cysteine) peptidase inhibitor, clade E, member 2
  • serpin peptidase inhibitor, clade B (ovalbumin), member 2
  • serpine1
  • SERPINE1
  • CpipJ_CPIJ011718
  • CpipJ_CPIJ016298
  • PAI-1
  • Serpine1
  • Serpine2
  • SERPINB2
Reactivity
Human
Alternatives
Kits with alternative reactivity to:
36
20
19
13
11
7
3
3
3
2
2
1
1
Method Type
Sandwich ELISA
Detection Range
12.5-800 pg/mL
Minimum Detection Limit
12.5 pg/mL
Application
ELISA
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Catalog No. ABIN1029562
$ 692.68
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Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
9.704079 ABIN415046 Sandwich ELISA Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 12.5-800 pg/mL 12.5 pg/mL Log in to see 2
9.704079 ABIN411404 Sandwich ELISA Cell Culture Supernatant, Cell Lysate, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate) 156-10000 pg/mL 156 pg/mL Log in to see 1
9.704079 ABIN612750 Sandwich ELISA Plasma, Cell Culture Supernatant 300 pg/mL Log in to see 11
9.704079 ABIN3198611 Sandwich ELISA Cell Culture Supernatant, Plasma, Serum 312.50-20000 pg/mL 312.50 pg/mL Log in to see
9.704079 ABIN1116528 Sandwich ELISA Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
9.704079 ABIN2748504 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 20-6.000 pg/mL 20 pg/mL Log in to see
9.704079 ABIN1379953 Sandwich ELISA Cell Lysate, Serum, Plasma 23-3000 pg/mL 23 pg/mL Log in to see
9.704079 ABIN456178 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Biological Fluids 0.156-10 ng/mL 0.156 ng/mL Log in to see
9.704079 ABIN2010754 Sandwich ELISA 0.25-100 ng/mL 0.25 ng/mL Log in to see 1
9.704079 ABIN2010722 Sandwich ELISA 0.125-100 U/mL 0.125 U/mL Log in to see 10
9.704079 ABIN625343 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 35 pg/mL-25 ng/mL 35 pg/mL Log in to see
9.704079 ABIN2748503 Sandwich ELISA Plasma, Cell Culture Supernatant, Serum 80-20.000 pg/mL 80 pg/mL Log in to see
9.704079 ABIN492021 Sandwich ELISA Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 2.74-2.000 pg/mL 2.74 pg/mL Log in to see
9.704079 ABIN2708354 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 156-10000 pg/mL 156 pg/mL Log in to see
9.704079 ABIN2708355 Sandwich ELISA Cell Culture Supernatant, Serum, Plasma, Tissue Homogenate 156-10000 pg/mL 156 pg/mL Log in to see
9.704079 ABIN2114761 Plasma, Tissue Lysate, Cell Culture Supernatant Log in to see
9.704079 ABIN5499278 Sandwich ELISA Cell Lysate 39-2500 pg/mL 39 pg/mL Log in to see
1 ABIN366579 Sandwich ELISA Serum, Plasma, Tissue Homogenate 3.125-200 ng/mL 3.125 ng/mL Log in to see 2
1 ABIN5594371 Sandwich ELISA Biological Fluids, Plasma, Serum, Tissue Homogenate 0.156-10 ng/mL 0.156 ng/mL Log in to see
1 ABIN511475 Sandwich ELISA Serum, Plasma, Cell Culture Supernatant, Tissue Homogenate, Body Fluids 250-5000 pg/mL 250 pg/mL Log in to see

Top referenced SERPINE1 ELISA Kit for ELISA

  • Human SERPINE1 ELISA Kit for Sandwich ELISA - ABIN2010722 (10 Publications): Balc? Ekmekçi, Ekmekçi, Güngör, Tüten, Toprak, Korkmaz, Öncül, Çal??kan, Kucur, Donma, Madazl?, Sönmez: Evaluation of Lp-PLA2 mass, vitronectin and PAI-1 activity levels in patients with preeclampsia. in Archives of gynecology and obstetrics 2015 (PubMed)

  • Human SERPINE1 ELISA Kit for Sandwich ELISA - ABIN612750 (11 Publications): Johansson, Sørensen, Perner, Welling, Wanscher, Larsen, Ostrowski: High sCD40L levels early after trauma are associated with enhanced shock, sympathoadrenal activation, tissue and endothelial damage, coagulopathy and mortality. in Journal of thrombosis and haemostasis : JTH 2012 (PubMed)

  • Human SERPINE1 ELISA Kit for Sandwich ELISA - ABIN415046 (2 Publications): Topaloglu, Arslan, Karakose, Ucan, Ginis, Cakir, Akkaymak, Sahin, Ozbek, Cakal, Delibasi: Is there any association between thrombosis and tissue factor pathway inhibitor levels in patients with vitamin D deficiency? in Clinical and applied thrombosis/hemostasis : official journal of the International Academy of Clinical and Applied Thrombosis/Hemostasis 2015 (PubMed)

  • Human SERPINE1 ELISA Kit for Sandwich ELISA - ABIN366579 (2 Publications): Zhang, Chen, Chen, Sun, Zeng, Chen, Liu, Cao, Ren, Wang: The association of new inflammatory markers with type 2 diabetes mellitus and macrovascular complications: a preliminary study. in European review for medical and pharmacological sciences 2014 (PubMed)

  • Human SERPINE1 ELISA Kit for Sandwich ELISA - ABIN2010754 (1 Publications): Bondu, Schrader, Gawinowicz, McGuire, Lawrence, Hjelle, Buranda: Elevated cytokines, thrombin and PAI-1 in severe HCPS patients due to Sin Nombre virus. in Viruses 2015 (PubMed)

  • Human SERPINE1 ELISA Kit for Sandwich ELISA - ABIN411404 (1 Publications): Chen, Lu, Yang, Lu, Chen, Zhang: Suppression of plasminogen activator inhibitor-1 by inhaled nitric oxide attenuates the adverse effects of hyperoxia in a rat model of acute lung injury. in Thrombosis research 2015 (PubMed)

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General

Antigen serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kits
Reactivity Human
Kits with alternative reactivity to:
(36), (20), (19), (13), (11), (7), (3), (3), (3), (2), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 12.5-800 pg/mL
Minimum Detection Limit 12.5 pg/mL
Application ELISA
Supplier Log in to see

Product Details SERPINE1 ELISA Kit

Target details Application Details Handling Images
Purpose The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of PAI1 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Sample Type Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This assay has high sensitivity and excellent specificity for detection of this index.
Cross-Reactivity (Details) No significant cross-reactivity or interference between this index and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between this index and all the analogues, therefore, cross reaction may still exist.
Sensitivity 4.9 pg/mL
Components
  • Pre-coated, ready to use 96-well strip plate
  • Standard (freeze dried)
  • Standard Diluent
  • Detection Reagent A
  • Detection Reagent B
  • Assay Diluent A
  • Assay Diluent B
  • TMB
  • Stop Solution
  • Wash Buffer (30X)
  • Plate sealer for 96 wells
  • Instruction manual
Material not included
  1. Microplate reader with 450 ± 10nm filter.
  2. Precision single or multi-channel pipettes and disposable tips.
  3. Eppendorf Tubes for diluting samples.
  4. Deionized or distilled water.
  5. Absorbent paper for blotting the microtiter plate.
  6. Container for Wash Solution.

Target details

Product Details SERPINE1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name PAI1 (SERPINE1 ELISA Kit Abstract)
Background Synonyms: Plasminogen activator inhibitor 1(PAI,PAI-1), Endothelial plasminogen activator inhibitor,Serpin E1,
Gene ID 5054
UniProt P05121
Pathways p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration

Application Details

Product Details SERPINE1 ELISA Kit Target details Handling Images back to top
Sample Volume 100 μL
Assay Time 1 - 4.5 h
Plate Pre-coated
Protocol 1. Prepare all reagents, samples and standards
2. Add 100 µL standard or sample to each well. Incubate 2 hours at 37 °C
3. Aspirate and add 100 µL prepared Detection Reagent A. Incubate 1 hour at 37 °C
4. Aspirate and wash 3 times
5. Add 100 µL prepared Detection Reagent B. Incubate 30 minutes at 37 °C
6. Aspirate and wash 5 times
7. Add 90 µL Substrate Solution. Incubate 15-25 minutes at 37 °C
8. Add 50 µL Stop Solution. Read at 450nm immediately.
Reagent Preparation Wash Buffer - If crystals have formed in the concentrate, warm to room temperature and mix gently until the crystals have completely dissolved. Dilute 30 mL of Wash Buffer Concentrate into deionized or distilled water to prepare 750 mL of Wash Buffer. Standard - Reconstitute the Standard with 1.0 mL of Sample Diluent. This reconstitution produces a stock solution of 10.0 ng/mL. Allow the standard to sit for a minimum of 15 minutes with gentle agitation prior to making serial dilutions (Making serial dilution in the wells directly is not permitted). The undiluted standard serves as the high standard (10.0 ng/mL). The Sample Diluent serves as the zero standard (0 ng/mL). 500ul 500ul 500ul 500ul 500ul 500ul Tube S7 S6 S5 S4 S3 S2 S1 S0 ng/mL 10.0 5.00 2.50 1.25 0.62 0.31 0.15 0 Detection Reagent A and B - Dilute to the working concentration using Assay Diluent A and B (1:100), respectively.
Sample Collection Serum - Use a serum separator tube (SST) and allow samples to clot for 30 minutes before centrifugation for 15 minutes at approximately 1000 × g. Remove serum and assay immediately or aliquot and store samples at -20 °C or -80 °C. Plasma - Collect plasma using EDTA or heparin as an anticoagulant. Centrifuge samples for 15 minutes at 1000 × g at 2 °C - 8 °C within 30 minutes of collection. Store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles. Urine - Aseptically collect the first urine of the day (mid-stream), voided directly into a sterile container. Centrifuge to remove particulate matter, assay immediately or aliquot and store at ≤ -20 °C. Avoid repeated freeze-thaw cycles. Tissue homogenates - The preparation of tissue homogenates will vary depending upon tissue type. For this assay, tissue was rinsed with 1X PBS to remove excess blood, homogenized in 20 mL of 1X PBS and stored overnight at ≤ -20 °C After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g. Remove the supernate and assay immediately or aliquot and store at ≤ -20 °C. Cell culture supernates and Other biological fluids - Remove particulates by centrifugation and assay immediately or aliquot and store samples at -20 °C or -80 °C. Avoid repeated freeze-thaw cycles.
Note:
1. Samples to be used within 5 days may be stored at 2-8 °C , otherwise samples must stored at -20 °C (1 month) or -80 °C (2 months) to avoid loss of bioactivity and contamination.
2. Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
3. Influenced by the factors including cell viability, cell number and also sampling time, samples from cell culture supernatant may not be detected by the kit
4. Sample hemolysis will influence the result, so hemolytic specimen can not be detected.
5. When performing the assay slowly bring samples to room temperature.
6. Do not use heat-treated specimens.
Sample Preparation Allow all reagents to reach room temperature (Please do not dissolve the reagents at 37 °C directly.). All the reagents should be mixed thoroughly by gently swirling before pipetting. Avoid foaming. Keep appropriate numbers of strips for 1 experiment and remove extra strips from microtiter plate. Removed strips should be resealed and stored at 4 °C until the kits expiry date. Prepare all reagents, working standards and samples as directed in the previous sections. Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
1. Add 100 µL of Standard, Blank, or Sample per well. Cover with the Plate sealer. Incubate for 2 hours at 37 °C.
2. Remove the liquid of each well, don’t wash. Add 100 µL of Detection Reagent A working solution to each well. Cover with the Plate sealer. Incubate for 1 hour at 37 °C. Detection Reagent A working solution may appear cloudy. Warm to room temperature and mix gently until solution appears uniform.
3. Aspirate each well and wash, repeating the process three times for a total of three washes. Wash by filling each well with Wash Buffer (approximately 400 µL) using a squirt bottle, multi-channel pipette, manifold dispenser or autowasher. and let it sit for 1~2 minutes. Complete removal of liquid at each step is essential to good performance. After the last wash, remove any remaining Wash Buffer by aspirating or decanting. Invert the plate and blot it against clean paper towels.
4. Add 100 µL of Detection Reagent B working solution to each well. Cover with a new Plate sealer. Incubate for 1 hour at 37 °C.
5. Repeat the aspiration/wash process for 5 times as conducted in step 3..
6. Add 90 µL of Substrate Solution to each well. Cover with a new Plate sealer. Incubate within 15-30 minutes at 37 °C. Protect from light.
7. Add 50 µL of Stop Solution to each well. If color change does not appear uniform, gently tap the plate to ensure thorough mixing.
8. Determine the optical density of each well at once, using a microplate reader set to 450 nm.
Note:
1. Absorbance is a function of the incubation time. Therefore, prior to starting the assay it is recommended that all reagents should be freshly prepared prior to use and all required strip-wells are secured in the microtiter frame. This will ensure equal elapsed time for each pipetting step, without interruption.
2. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals have completely dissolved. The reconstituted Standards Detection Reagent A and B can be used only once. This assay requires pipetting of small volumes. To minimize imprecision caused by pipetting, ensure that pipettors are calibrated. It is recommended to suck more than 10µL for once pipetting.
3. To ensure accurate results, proper adhesion of plate sealers during incubation steps is necessary. Do not allow wells to sit uncovered for extended periods between incubation steps. Once reagents have been added to the well strips, DO NOT let the strips DRY at any time during the assay.
4. For each step in the procedure, total dispensing time for addition of reagents to the assay plate should not exceed 10 minutes.
5. To avoid cross-contamination, change pipette tips between additions of each standard level, between sample additions, and between reagent additions. Also, use separate reservoirs for each reagent.
6. The wash procedure is critical. Insufficient washing will result in poor precision and falsely elevated absorbance readings.
7. Duplication of all standards and specimens, although not required, is recommended.
8. Substrate Solution is easily contaminated. Please protect it from light.
9. The web version of manual is only for reference, subject to the instruction shipping with the kit.
Assay Procedure The microtiter plate provided in this kit has been pre-coated with an antibody specific to the index. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to the index. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain the index, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of the index in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Calculation of Results Average the duplicate readings for each standard, control, and sample and subtract the average zero standard optical density. Create a standard curve by reducing the data using computer software capable of generating a four parameter logistic (4-PL) curve-fit. As an alternative, construct a standard curve by plotting the mean absorbance for each standard on the x-axis against the concentration on the y-axis and draw a best fit curve through the points on the graph. The data may be linearized by plotting the log of the PAI concentrations versus the log of the O.D. and the best fit line can be determined by regression analysis. It is recommended to use some related software to do this calculation, such as curve expert 1.3. This procedure will produce an adequate but less precise fit of the data. If samples have been diluted, the concentration read from the standard curve must be multiplied by the dilution factor.
Important note:
1. Limited by the current condition and scientific technology, we can't completely conduct the comprehensive identification and analysis on the raw material provided by suppliers. So there might be some qualitative and technical risks to use the kit
2. The final experimental results will be closely related to validity of the products, operation skills of the end users and the experimental environments. Please make sure that sufficient samples are available.
3. Kits from different batches may be a little different in detection range, sensitivity and color developing time.Please perform the experiment exactly according to the instruction attached in kit while electronic ones from our website is only for information.
4. There may be some foggy substance in the wells when the plate is opened at the first time. It will not have any effect on the final assay results.
5. Do not remove microtiter plate from the storage bag until needed.
6. A microtiter plate reader with a bandwidth of 10nm or less and an optical density range of 0-3 OD or greater at 450nm wavelength is acceptable for use in absorbance measurement.
7. Use fresh disposable pipette tips for each transfer to avoid contamination.
8. Do not substitute reagents from one kit lot to another. Use only the reagents supplied by manufacturer.
9. Even the same operator might get different results in two separate experiments. In order to get better reproducible results, the operation of every step in the assay should be controlled. Furthermore, a preliminary experiment before assay for each batch is recommended.
10. Each kit has been strictly passed Q.C test. However, results from end users might be inconsistent with our in-house data due to some unexpected transportation conditions or different lab equipments. Intra-assay variance among kits from different batches might arise from above factors, too.
11. Kits from different manufacturers for the same item might produce different results, since we haven’t compared our products with other manufacturers.
12. The instruction manual also suit for the kit of 48T, but all reagents of 48T kit is reduced by half.
13. Valid period: six months.
Assay Precision
  • Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level the index were tested 20 times on one plate, respectively.
  • Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level the index were tested on 3 different plates, 8 replicates in each plate.
  • CV(%) = SD/meanX100
  • Intra-assay: CV&lt10%
  • Inter-assay: CV&lt12%
Restrictions For Research Use only

Handling

Product Details SERPINE1 ELISA Kit Target details Application Details Images back to top
Precaution of Use The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.
Handling Advice The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage conditions. Note: To minimize unnecessary influences on the performance, operation procedures and lab conditions, especially room temperature, air humidity and incubator temperatures should be strictly regulated. It is also strongly suggested that the whole assay is performed by the same experimenter from the beginning to the end.
Storage 4 °C,-20 °C
Storage Comment The Assay Plate, Standard, Detection Reagent A and Detection Reagent B should be stored at -20°C upon being received. After receiving the kit , Substrate should be always stored at 4°C.Other reagents are kept according to the labels on vials. But for long term storage, please keep the whole kit at -20°C. The unused strips should be kept in a sealed bag with the desiccant provided to minimize exposure to damp air. The test kit may be used throughout the expiration date of the kit (six months from the date of manufacture). Opened test kits will remain stable until the expiring date shown, provided it is stored as prescribed above.
Expiry Date 12 months

Images

Product Details SERPINE1 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for SERPINE1 ELISA Kit (serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1) (ABIN1029562) serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kit