Rhesus Monkey SERPINE1 ELISA Kit

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Antigen
serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kits
  • PAI
  • PAI-1
  • PAI1
  • PLANH1
  • si:ch211-138a11.1
  • SERPINE1
  • Planh1
  • B230326M24Rik
  • PI-7
  • PI7
  • PN-1
  • Spi4
  • HsT1201
  • PAI-2
  • PAI2
  • PLANH2
  • PAI1A
  • Pai1
  • Pai1aa
  • Planh
  • RATPAI1A
  • serpin peptidase inhibitor, clade E (nexin, plasminogen activator inhibitor type 1), member 1
  • plasminogen activator inhibitor 1
  • plasminogen activator inhibitor type 1
  • serine (or cysteine) peptidase inhibitor, clade E, member 1
  • serine (or cysteine) peptidase inhibitor, clade E, member 2
  • serpin peptidase inhibitor, clade B (ovalbumin), member 2
  • serpine1
  • SERPINE1
  • CpipJ_CPIJ011718
  • CpipJ_CPIJ016298
  • PAI-1
  • Serpine1
  • Serpine2
  • SERPINB2
Reactivity
Human, Mouse (Murine), Rhesus Monkey
Alternatives
Kits with alternative reactivity to:
39
22
18
14
11
7
3
3
3
2
2
1
1
Method Type
Sandwich ELISA
Detection Range
20-6.000 pg/mL
Minimum Detection Limit
20 pg/mL
Application
ELISA
Options
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Catalog No. ABIN2748504
$ 666.60
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
10.090268 ABIN415321 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.31-20 ng/mL 0.31 ng/mL Log in to see
1 ABIN2851423 Sandwich ELISA Serum, Plasma, Tissue Homogenate, Cell Lysate, Cell Culture Supernatant, Biological Fluids 0.312-20 ng/mL 0.312 ng/mL Log in to see
1 ABIN5657857 Sandwich ELISA Biological Fluids, Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate 0.312 ng/mL - 20 ng/mL 0.312 ng/mL Log in to see

General

Antigen serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kits
Reactivity Human, Mouse (Murine), Rhesus Monkey
Kits with alternative reactivity to:
(39), (22), (18), (14), (11), (7), (3), (3), (3), (2), (2), (1), (1)
Method Type Sandwich ELISA
Detection Range 20-6.000 pg/mL
Minimum Detection Limit 20 pg/mL
Application ELISA
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Product Details SERPINE1 ELISA Kit

Target details Application Details Handling Images
Purpose Rhesus Monkey PAI-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Sample Type Plasma, Cell Culture Supernatant, Serum
Detection Method Colorimetric
Analytical Method Quantitative
Specificity This ELISA antibody pair detects Rhesus Monkey, mouse and human PAI-1.
Sensitivity 20 pg/mL
Characteristics
  • Strip plates and additional reagents allow for use in multiple experiments
  • Quantitative protein detection
  • Establishes normal range
  • The best products for confirmation of antibody array data
Components
  • Pre-Coated 96-well Strip Microplate
  • Wash Buffer
  • Stop Solution
  • Assay Diluent(s)
  • Lyophilized Standard
  • Biotinylated Detection Antibody
  • Streptavidin-Conjugated HRP
  • TMB One-Step Substrate
Material not included
  • Distilled or deionized water
  • Precision pipettes to deliver 2 μL to 1 μL volumes
  • Adjustable 1-25 μL pipettes for reagent preparation
  • 100 μL and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis

Target details

Product Details SERPINE1 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name PAI-1 (SERPINE1 ELISA Kit Abstract)
Background Plasminogen activator inhibitor-1
Gene ID 713883
UniProt F6Y5D7
Pathways p53 Signaling, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Autophagy, Smooth Muscle Cell Migration

Application Details

Product Details SERPINE1 ELISA Kit Target details Handling Images back to top
Application Notes Recommended Dilution for serum and plasma samples100 fold
Sample Volume 100 μL
Plate Pre-coated
Protocol
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 μL of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4 °C.
  4. Add 100 μL of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 μL of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 μL of Stop Solution to each well.
  11. Read at 450 nm immediately.
Assay Procedure Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.
Restrictions For Research Use only

Handling

Product Details SERPINE1 ELISA Kit Target details Application Details Images back to top
Storage -20 °C
Storage Comment The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Expiry Date 6 months

Images

Product Details SERPINE1 ELISA Kit Target details Application Details Handling back to top
Supplier Images
ELISA image for serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kit (ABIN2748504) serpin Peptidase Inhibitor, Clade E (Nexin, Plasminogen Activator Inhibitor Type 1), Member 1 (SERPINE1) ELISA Kit