Mouse (Murine) TBX2 ELISA Kit

Recommended TBX2 Product (supplied by: Log in to see )

Antigen
T-Box 2 (TBX2) ELISA Kits
  • Xltbx2
  • tbx2
  • xtbx2
  • hm:zeh1581
  • tbx-c
  • tbxc
  • zeh1581
  • TBX2
  • T-box 2
  • T-box 2b
  • Protein TBX-2
  • TBX2
  • tbx2-a
  • tbx2b
  • tbx2
  • tbx-2
  • Tbx2
Reactivity
Human, Mouse (Murine)
Alternatives
Kits with alternative reactivity to:
5
1
1
Method Type
DNA-Binding ELISA
Application
ELISA
Options
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Catalog No. ABIN2114991
$ 610.39
Plus shipping costs $45.00
Relevance Score ABIN Method Type Sample Type Detection Range Detection Minimum Supplier References Details
1 ABIN2114990 Cell ELISA Log in to see

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General

Antigen T-Box 2 (TBX2) ELISA Kits
Reactivity Human, Mouse (Murine)
Kits with alternative reactivity to:
(5), (1), (1)
Method Type DNA-Binding ELISA
Application ELISA
Supplier Log in to see

Product Details TBX2 ELISA Kit

Target details Application Details Handling Images
Detection Method Colorimetric
Analytical Method Quantitative
Specificity The TBX2 DNA-Binding ELISA Kit detects endogenous levels of total TBX2 protein.
Characteristics Assay Type: DNA-Binding

Target details

Product Details TBX2 ELISA Kit Application Details Handling Images back to top
Antigen
Alternative Name TBX2 (TBX2 ELISA Kit Abstract)
Background Synonyms: T-box transcription factor TBX2 , T-box protein 2, TBX2
Gene Symbol: TBX2
Gene ID 6909
UniProt Q13207
Pathways p53 Signaling

Application Details

Product Details TBX2 ELISA Kit Target details Handling Images back to top
Plate Pre-coated
Assay Procedure
  • Remove the coating solution and wash the plate three times by filling the wells with 100 μL PBS-0.05 % Tween20. The solutions or washes are removed by flicking the plate over a sink. The remaining drops are removed by patting the plate on a paper towel.
  • Block the remaining protein-binding sites in the coated wells by adding 100 μL blocking buffer, 3 % skim milk in PBS per well. Incubate for 1 hour at RT with gentle shaking.
  • Wash the plate three times with 100 μL PBS-0.05 % Tween 20.
  • Add 50 μL of diluted antibody to each well. Incubate the plate at 37 °C for an hour with gentle shaking.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween 20.
  • Add 50 μL of conjugated secondary antibody, diluted at the optimal concentration (according to the manufacturer) in blocking buffer immediately before use. Incubate at 37 °C for an hour.
  • Wash the plate six times with 100 μL PBS-0.05 %Tween20.
  • Prepare the substrate solution by mixing acetic acid, TMB and 0.03 % H2O2 with the volume ratio of 4:1:5.
  • Dispense 50 μL of the substrate solution per well with a multichannel pipe. Incubate the plate at 37 °C in dark for 15-30 mins.
  • After sufficient color development, add 100 μL of stop solution to the wells (if necessary).
  • Read the absorbance (optical density at 450nm) of each well with a plate reader.
Restrictions For Research Use only

Handling

Product Details TBX2 ELISA Kit Target details Application Details Images back to top
Handling Advice Avoid multiple freeze-thaw cycles
Storage 4 °C
Storage Comment Store at 4 °C for frequent use, at -20°C for infrequent use.
Expiry Date 6 months