Human HPRG / HRG ELISA Pair Set

Details for Product No. ABIN2212586, Supplier: Log in to see
Antigen
  • NRG1
  • wu:fm70e01
  • DKFZp459A1838
  • HPRG
  • HRGP
  • THPH11
  • HRG1
  • ARIA
  • GGF
  • GGF2
  • HGL
  • HRG
  • HRGA
  • MST131
  • NDF
  • SMDF
  • 6030402G23Rik
  • D230005F13Rik
  • GGFII
  • HRGalpha
  • Hgl
  • pro-neuregulin-1
  • NRG-1
  • heregulin
  • nrg1-A
  • neuregulin 1
  • histidine-rich glycoprotein
  • NRG1
  • nrg1
  • Nrg1
  • HRG
  • Hrg
Reactivity
Human
Host
Mouse
Conjugate
HRP
Method Type
Sandwich ELISA
Application
ELISA
Options
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Purpose The human HPRG / HRG ELISA Pair Set is for the quantitative determination of human HPRG / HRG.
This ELISA Pair Set contains the basic components required for the development of sandwich ELISAs.
Detection Method Colorimetric
Sensitivity 18.75 pg/mL
Characteristics Capture Antibody: 0.4 mg/mL of mouse anti-HPRG monoclonal antibody (in PBS, pH 7.4). Dilute to a working concentration of 2 μg/mL in PBS before coating.

Detection Antibody: 0.2 mg/mL rabbit anti-HPRG polyclonal antibody conjugated to horseradish-peroxidase (HRP) (in PBS, 50 % HRP-protector, pH 7.4). Dilute to working concentration of 0.7 μg/mL in detection antibody dilution buffer before use.

Standard: Each vial contains 10 ng of recombinant HPRG. Reconstitute with 1 mL detection antibody dilution buffer. After reconstitution, store at -20 °C to -80 °C in a manual defrost freezer. A seven-point standard curve using 2-fold serial dilutions in sample dilution buffer, and a high standard of 1.2 ng/mL is recommended.

Sensitivity: The minimum detectable dose of human HPRG / HRG was determined to be approximately 18.75 pg/mL. This is defined as at least three times standard deviations above the mean optical density of 10 replicates of the zero standard.
Components
  • Capture Ab
  • Detection Ab
  • Standard
Alternative Name HRG
Research Area Cardiovascular, Angiogenesis
Application Notes Optimal working dilution should be determined by the investigator.
Protocol This ELISA Pair Set is a solid phase sandwich ELISA (Enzyme-Linked Immunosorbent Assay). It utilizes a monoclonal antibody specific for HPRG / HRGcoated on a 96-well plate. Standards and samples are added to the wells, andany HPRG / HRG present binds to the immobilized antibody. The wells are washedand a horseradish peroxidase conjugated rabbit anti-HPRG / HRG polyclonal antibody is then added, producing an antibody-antigen-antibody "sandwich". Thewells are again washed and TMB substrate solution is loaded, which producescolor in proportion to the amount of HPRG / HRG present in the sample. To endthe enzyme reaction, the stop solution is added and absorbances of themicrowell are read at 450 nm.
Restrictions For Research Use only
Format Liquid
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C,-80 °C
Storage Comment Capture Antibody: Aliquot and store at -20°C to -80°C for up to 6 months from date of receipt. Avoid repeated freeze-thaw cycles.

Detection Antibody: Store at 4°C and protect it from prolonged exposure to light for up to 6 months from date of receipt. DO NOT FREEZE!

Standard: Store lyophilized standard at -20°C to -80°C for up to 6 months from date of receipt. Aliquot and store the reconstituted Standard at -80°C for up to 1 month. Avoid repeated freeze-thaw cycles.
Supplier Images
ELISA image for Human HPRG / HRG ELISA Pair Set (ABIN2212586) Human HPRG / HRG ELISA Pair Set
Product cited in: Deuschle, Birkel, Hambruch, Hornberger, Kinzel, Perovi?-Ottstadt, Schulz, Hahn, Burnet, Kremoser: "The nuclear bile acid receptor FXR controls the liver derived tumor suppressor histidine-rich glycoprotein." in: International journal of cancer. Journal international du cancer, Vol. 136, Issue 11, pp. 2693-704, 2015 (PubMed).