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FLIPs (C-Term) Peptide

Details for Product No. ABIN1003794, Supplier: Log in to see
Protein Name
Protein Region
Western Blotting (WB)
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Supplier Product No.
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Blocked Antibody anti-FLICE Inhibitory Protein (CFLAR) (C-Term) antibody (ABIN1002320)
Characteristics 19 amino acids near the carboxy terminus of human FLIP.
Application Notes FLIP peptide is used for blocking the activity of Anti-FLIP . Incubating the peptide with equal volume of FLIP antibody for 30 min at 37ºC usually completely blocks the antibody activity in Western blotting.
Restrictions For Research Use only
Format Liquid
Concentration 200 µg/mL
Buffer Supplied in PBS pH 7.2 (10 mM NaH₂PO₄, 10 mM Na₂HPO₄, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. The peptide solution should be gently mixed before use.
Storage -20 °C
Storage Comment Store at -20°C, stable for one year.
Expiry Date 12 months
Background publications Cecconi, Alvarez-Bolado, Meyer, Roth, Gruss: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998

Hu, Benedict, Wu, Inohara, Núñez: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998

Zou, Henzel, Liu, Lutschg, Wang: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997

Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997