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Tripartite Motif Containing 5 (TRIM5) (Internal Region) Peptide

Details for Product No. ABIN1004059, Supplier: Log in to see
Protein Name
  • EG667823
  • Gm8833
  • RGD1304579
  • RNF88
  • TRIM5
  • TRIM5alpha
Protein Region
Internal Region
Application
Western Blotting (WB)
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Blocked Antibody anti-TRIM5 antibody (Tripartite Motif Containing 5) (Internal Region) (ABIN1003376)
Characteristics 15 amino acids from towards the carboxy terminus of human TRIM5.
Application Notes TRIM5 alpha peptide is used for blocking the activity of the TRIM5 alpha (IN1) antibody . Incubating the peptide with equal volume of antibody for 30 min at 37ºC usually completely blocks the antibody activity in Western blotting.
Restrictions For Research Use only
Format Liquid
Concentration 200 µg/mL
Buffer Supplied in PBS pH 7.2 (10 mM NaH₂PO₄, 10 mM Na₂HPO₄, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. The peptide solution should be gently mixed before use.
Storage -20 °C
Storage Comment Store at -20°C, stable for one year.
Expiry Date 12 months
Product cited in: Gaide, Martinon, Micheau, Bonnet, Thome, Tschopp: "Carma1, a CARD-containing binding partner of Bcl10, induces Bcl10 phosphorylation and NF-kappaB activation." in: FEBS letters, Vol. 496, Issue 2-3, pp. 121-7, 2001

McAllister-Lucas, Inohara, Lucas, Ruland, Benito, Li, Chen, Chen, Yamaoka, Verma, Mak, Núñez: "Bimp1, a MAGUK family member linking protein kinase C activation to Bcl10-mediated NF-kappaB induction." in: The Journal of biological chemistry, Vol. 276, Issue 33, pp. 30589-97, 2001

Fanning, Anderson: "Protein modules as organizers of membrane structure." in: Current opinion in cell biology, Vol. 11, Issue 4, pp. 432-9, 1999

Background publications Bertin, Wang, Guo, Jacobson, Poyet, Srinivasula, Merriam, DiStefano, Alnemri: "CARD11 and CARD14 are novel caspase recruitment domain (CARD)/membrane-associated guanylate kinase (MAGUK) family members that interact with BCL10 and activate NF-kappa B." in: The Journal of biological chemistry, Vol. 276, Issue 15, pp. 11877-82, 2001