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Caspase-4 / ICH-2 / Mih1 (N-Term) Peptide

Details for Product No. ABIN1004108, Supplier: Login to see
Protein Name
Protein Region
N-Term
Application
Western Blotting (WB)
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Blocked Antibody anti-Caspase 2 (Induced By ER Stress) (N-Term) antibody (ABIN1002006)
Characteristics 16 amino acids near the amino terminus of human caspase-4.
Application Notes Caspase-4 peptide is used for blocking the activity of Anti-caspase-4 antibody . It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37ºC.
Restrictions For Research Use only
Format Liquid
Concentration 200 µg/mL
Buffer Supplied in PBS pH 7.2 (10 mM NaH₂PO₄, 10 mM Na₂HPO₄, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. The peptide solution should be gently mixed before use.
Storage -20 °C
Storage Comment Store at -20°C, stable for one year.
Expiry Date 12 months
Product cited in: Leaman, Chawla-Sarkar, Vyas et al.: "Identification of X-linked inhibitor of apoptosis-associated factor-1 as an interferon-stimulated gene that augments TRAIL Apo2L-induced apoptosis." in: The Journal of biological chemistry, Vol. 277, Issue 32, pp. 28504-11, 2002 (PubMed).

Fong, Liston, Rajcan-Separovic et al.: "Expression and genetic analysis of XIAP-associated factor 1 (XAF1) in cancer cell lines." in: Genomics, Vol. 70, Issue 1, pp. 113-22, 2001 (PubMed).

Background publications Liston, Fong, Kelly et al.: "Identification of XAF1 as an antagonist of XIAP anti-Caspase activity." in: Nature cell biology, Vol. 3, Issue 2, pp. 128-33, 2001 (PubMed).

Deveraux, Takahashi, Salvesen et al.: "X-linked IAP is a direct inhibitor of cell-death proteases." in: Nature, Vol. 388, Issue 6639, pp. 300-4, 1997 (PubMed).