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Toll-Like Receptor Adaptor Molecule 1 (TICAM1) Peptide

Details for Product No. ABIN1004485, Supplier: Log in to see
Protein Name
  • AW046014
  • AW547018
  • IIAE6
  • MyD88-3
  • PRVTIRB
  • TICAM-1
  • trif
  • TRIF
Application
Western Blotting (WB)
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Blocked Antibody anti-TRIF (Toll-Like Receptor Adaptor Protein) antibody (ABIN1003373)
Characteristics 14 amino acids near the carboxy terminus of human TRIF.
Application Notes TRIF peptide is used for blocking the activity of the Anti-TRIF antibody . It usually blocks the antibody activity completely in Western blot by incubating the peptide with equal volume of antibody for 30 min at 37ºC.
Restrictions For Research Use only
Format Liquid
Concentration 200 µg/mL
Buffer Supplied in PBS pH 7.2 (10 mM NaH₂PO₄, 10 mM Na₂HPO₄, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice For products with volumes of 200 μl or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container’s cap. The peptide solution should be gently mixed before use.
Storage -20 °C
Storage Comment Store at -20°C, stable for one year.
Expiry Date 12 months
Product cited in: Poyet, Srinivasula, Tnani, Razmara, Fernandes-Alnemri, Alnemri: "Identification of Ipaf, a human caspase-1-activating protein related to Apaf-1." in: The Journal of biological chemistry, Vol. 276, Issue 30, pp. 28309-13, 2001

Geddes, Wang, Huang, Lavellee, Manji, Brown, Jurman, Cao, Morgenstern, Merriam, Glucksmann, DiStefano, Bertin: "Human CARD12 is a novel CED4/Apaf-1 family member that induces apoptosis." in: Biochemical and biophysical research communications, Vol. 284, Issue 1, pp. 77-82, 2001

Background publications Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997