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ADP-Ribosylation Factor 1 (ARF1) Peptide
| Name | ADP-Ribosylation Factor 1 (ARF1) |
| Synonyms |
ARD1, ARFD1, RNF46, ARF-1, ARF1, Arf, Darf1, Dm Arf79F, dARFI, DmelCG8385, CG8385, MGC72830, ARF7, FLJ22595, wu:fb33e02, wu:fb78h01, arf2, MGC52628, arf-1, MGC52573, TRIM23, ADP-RIBOSYLATION FACTOR, A ...
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| Catalog no. | ABIN492784 |
| Quantity | 0.25 mg |
| Price | 195.00 $ Plus shipping costs $35.00 |
| Shipping to |
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| Availability | Ships within 7 to 10 Business Days |
Additional Information
| Gene ID | 64310 |
| Swiss-Prot | P84079 |
| Description | Ras superfamily of small GTPases regulates a wide variety of cellular processes includingintracellular trafficking of proteins. The Ras proteins acts as molecular switches by cyclingbetween inactive GDP-bound and active GTP-bound state. Guanine exchange factors (GEFs)Among the various Ras proteins, the ADP-ribosylation factor proteins (ARFs) are wellcharacterized regulators of vesicle formation in intracellular membrane traffic while Rhoproteins regulate the actin cytoskeleton (1). The Arf family members have unique structuraldevice, called the ‘interswitch toggle’, that implements communication between thenucleotide-binding site and a unique N-terminal amphipathic helix that mediates theinteraction of Arf proteins with membranes, these structural motifs distinguish them fromother small GTPases (2). When GDP is bound to ARF proteins the amphipathic helix ispositioned in a hydrophobic pocket, whereas in the GTP-bound forms it is displaced by theinterswitch toggle and stabilizes interaction of the protein with membrane lipid bilayer. Thus, the GTP-GDP cycle is coupled to membrane-cytosol cycle and thereby ARF proteinsare able to regulate vesicular traffic and organelle structure formation (2). The mammalian ARF family comprise of 5 members (ARF1-6), there is no ARF2. ARF1 andARF6 are most abundantly expressed and located in distinct cellular locations at Golgicomplex and early-endosome-plasma membrane interface and are most distantly relatedwith only 67% sequence identity. ARF1 is mostly known for its role in vesicle budding andGolgi regulation through the recruitment of coat proteins and in the regulation oflipid-modifying enzymes and actin organizing components (3). The crystal structure ofARF-BD (ARF binding domain) comprise of a PH domain adjacent to a C-terminal helix andARF interacts with these two regions via its “interswitch toggle” (4). ARF1 protein has 188amino acids and is expressed in at least 3 variant forms, ARF1 antibodies will label allvariants equally. The deactivation of ARF1 induces redistribution of entire Golgi complex toER, the GTP bound form triggers the association with coat protein with the Golgimembranes. Purified ARF proteins do not have GTPase activity suggesting that GTPhydrolysis is dependent on GTPase activating protein (GAP) a 49kDa protein (4). The GTPbound ARF1 and 3 proteins also interact with several interacting protein (ARFGAPs)including protein interacting with C kinase 1 (PICK1) to mediate their cellular processes. |
| Synonyms | ARD1, ARFD1, RNF46, ARF-1, ARF1, Arf, Darf1, Dm Arf79F, dARFI, DmelCG8385, CG8385, MGC72830, ARF7, FLJ22595, wu:fb33e02, wu:fb78h01, arf2, MGC52628, arf-1, MGC52573, TRIM23, ADP-RIBOSYLATION FACTOR, ADP-RIBOSYLATION FACTOR 1, ADP-RIBOSYLATION FACTOR 1A, ATARF, ATARF1, ATARFA1A, F28C11.12, arf1, A2MP, DKFZp564M0264, AI788669, AW496366, MGC102530, ARFGAP1, FLJ45618, ARF1GAP, MGC39924, HRIHFB2281, Arfgap1, 0610009H19Rik, 1810004P07Rik, 1810035F16Rik, 9130416J18Rik, Arf1gap, AI115377, Arf GAP1, MGC53624, arf6, MGC76053, MGC80156, arfgap1, MGC83573, MGC79593, ARFGAP3, ARF1A1C, T8I13.1 |
Application Details
| Storage | Store (in aliquots) at -20 °C. Avoid repeated freezing and thawing. Shelf life: one year from despatch. |
| Research Area | Cell Cycle |
| Restrictions | For Research Use only |
