BCL2-Interacting Killer (Apoptosis-Inducing) (BIK) Peptide

Details for Product No. ABIN499466
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Protein Name
Origin
Human
(1), (1)
Source
Synthetic
Application
Control Peptide (CP)
Pubmed 4 references available
Quantity 50 μg
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Availability Discontinued
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Catalog No. ABIN499466
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Specificity 15 amino acids near the amino terminus of human Bik.
Purification purified
Alternative Name BIK
Background Synonyms: Apoptosis inducer NBK, BIP1, BP4, Bcl-2-interacting killer, NBK
Gene ID 638
NCBI Accession NP_001188
UniProt Q13323
Application Notes Bik peptide is used for blocking the activity of Bik antibody. It usually blocks the antibodyactivity completely in Western blot by incubating the peptide with equal volume ofantibody for 30 min at 37 °C.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1 % bovine serum albumin and 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20 °C.
Background publications Biancone, Bowen, Lim et al.: "Identification of a novel inducible cell-surface ligand of CD5 on activated lymphocytes." in: The Journal of experimental medicine, Vol. 184, Issue 3, pp. 811-9, 1997 (PubMed).

Miyazaki, Hirokami, Matsuhashi et al.: "Increased susceptibility of thymocytes to apoptosis in mice lacking AIM, a novel murine macrophage-derived soluble factor belonging to the scavenger receptor cysteine-rich domain superfamily." in: The Journal of experimental medicine, Vol. 189, Issue 2, pp. 413-22, 1999 (PubMed).

Haruta, Kato, Hashimoto et al.: "Association of AIM, a novel apoptosis inhibitory factor, with hepatitis via supporting macrophage survival and enhancing phagocytotic function of macrophages." in: The Journal of biological chemistry, Vol. 276, Issue 25, pp. 22910-4, 2001 (PubMed).

Kuwata, Watanabe, Jiang et al.: "AIM inhibits apoptosis of T cells and NKT cells in Corynebacterium-induced granuloma formation in mice." in: The American journal of pathology, Vol. 162, Issue 3, pp. 837-47, 2003 (PubMed).

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