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Caspase 12 (CASP12) Peptide

Details for Product No. ABIN499550
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Protein Name
Origin
Mouse (Murine)
(2)
Application
Control Peptide (CP)
Pubmed 5 references available
Quantity 50 µg
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Availability Discontinued
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Catalog No. ABIN499550
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Specificity 18 amino acids within the large cleavage product of murine caspase-12.
Alternative Name Caspase-12
Background Alternate names: CASP-12, CASP12, Inactive caspase-12
Gene ID 12364
NCBI Accession NP_033938.3
UniProt O08736
Research Area Apoptosis/Necrosis
Application Notes Caspase-12 peptide is used for blocking the activity of the Caspase-12 (large) antibody. Itusually blocks the antibody activity completely in Western blots by incubating the peptidewith equal volume of antibody for 30 min at 37°C. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1% bovine serum albumin and 0.02% sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20°C.
Expiry Date 12 months
Background publications Molloy, Bresnahan, Leppla et al.: "Human furin is a calcium-dependent serine endoprotease that recognizes the sequence Arg-X-X-Arg and efficiently cleaves anthrax toxin protective antigen." in: The Journal of biological chemistry, Vol. 267, Issue 23, pp. 16396-402, 1992 (PubMed).

Cross, Lindsey, Fantes et al.: "The structure of a subterminal repeated sequence present on many human chromosomes." in: Nucleic acids research, Vol. 18, Issue 22, pp. 6649-57, 1991 (PubMed).

Duesbery, Webb, Leppla et al.: "Proteolytic inactivation of MAP-kinase-kinase by anthrax lethal factor." in: Science (New York, N.Y.), Vol. 280, Issue 5364, pp. 734-7, 1998 (PubMed).

Carson-Walter, Watkins, Nanda et al.: "Cell surface tumor endothelial markers are conserved in mice and humans." in: Cancer research, Vol. 61, Issue 18, pp. 6649-55, 2001 (PubMed).

Bradley, Mogridge, Mourez et al.: "Identification of the cellular receptor for anthrax toxin." in: Nature, Vol. 414, Issue 6860, pp. 225-9, 2001 (PubMed).

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