Lysine (K)-Specific Demethylase 1A (KDM1A) Peptide

Details for Product No. ABIN500199
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Protein Name
Origin
Human
(4)
Source
Synthetic
Application
Control Peptide (CP)
Pubmed 4 references available
Quantity 50 μg
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Catalog No. ABIN500199
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Specificity 16 amino acids near the center of human LSD1.
Purification purified
Alternative Name AOF2
Background Alternate names: BRAF35-HDAC complex protein BHC110, Flavin-containing amine oxidase domain- containing protein 2, KIAA0601, LSD1, Lysine-specific histone demethylase 1
Gene ID 23028
NCBI Accession NP_055828
UniProt O60341
Application Notes LSD1 peptide is used for blocking the activity of LSD1 antibody. It usually blocks theantibody activity completely in Western blot by incubating the peptide with equal volumeof antibody for 30 min at 37°C. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Concentration 0.2 mg/mL
Buffer PBS pH7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1 % bovine serum albumin and 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store at -20 °C.
Expiry Date 12 months
Background publications Little, Ramakrishnan, Roy et al.: "The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications." in: Critical reviews in eukaryotic gene expression, Vol. 4, Issue 1, pp. 1-18, 1995 (PubMed).

Haze, Yoshida, Yanagi et al.: "Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3787-99, 1999 (PubMed).

Yoshida, Matsui, Yamamoto et al.: "XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor." in: Cell, Vol. 107, Issue 7, pp. 881-91, 2002 (PubMed).

Calfon, Zeng, Urano et al.: "IRE1 couples endoplasmic reticulum load to secretory capacity by processing the XBP-1 mRNA." in: Nature, Vol. 415, Issue 6867, pp. 92-6, 2002 (PubMed).

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