Second Mitochondria-Derived Activator of Caspase (DIABLO) Peptide

Details for Product No. ABIN500783
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Protein Name
Origin
Mouse (Murine)
(7), (1)
Source
Synthetic
Application
Control Peptide (CP)
Pubmed 2 references available
Quantity 50 μg
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Catalog No. ABIN500783
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Specificity Control peptide for AP30815PU-N. Peptide consists of 16 amino acids near the carboxy terminus of mouse Smac.
Alternative Name DIABLO / SMAC
Background Synonyms: Diablo homolog mitochondrial, Direct IAP-binding protein with low pI, Second mitochondria-derived activator of caspase
Gene ID 66593
NCBI Accession NP_075721
UniProt Q9JIQ3
Application Notes Smac peptide is used for blocking the antibody activity of mSmac/DIABLO. It usuallyblocks the antibody activity completely in Western blot by incubating the peptide withequal volume of antibody for 30 min at 37 °C
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 0.2 mg/mL
Buffer PBS pH 7.2 (10 mM NaH2PO4, 10 mM Na2HPO4, 130 mM NaCl) containing 0.1 % bovine serum albumin and 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store (in aliquots) at -20 °C.
Background publications Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).

Tanaka, Arakawa, Yamaguchi et al.: "A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage." in: Nature, Vol. 404, Issue 6773, pp. 42-9, 2000 (PubMed).

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