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PYD and CARD Domain Containing (PYCARD) (C-Term) peptide (Biotin)

PYCARD Reactivity: Human Host: Synthetic BP, Imm
Catalog No. ABIN5539434
  • Target See all PYCARD products
    PYCARD (PYD and CARD Domain Containing (PYCARD))
    Protein Region
    C-Term
    Origin
    Human
    Source
    • 8
    Synthetic
    Purification tag / Conjugate
    This PYCARD peptide is labelled with Biotin.
    Application
    Blocking Peptide (BP), Immunogen (Imm)
    Sequence
    CRESQSYLVE DLERS.
    Predicted Reactivity
    Human
  • Application Notes
    When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 24 kDa band observed in lysates of cell line U937 (calculated MW of 21.6 kDa according to NP_037390.2). See non-biotinylated parental product's datasheet for further QC data. Recommended concentration: 1-3 μg/mL.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
    Buffer
    100µg of Lyophilised peptide, formulation De-ionized (DI) water.
    Storage
    -20 °C
    Storage Comment
    Shipped at ambient temperature, store at -20°C.
  • Target
    PYCARD (PYD and CARD Domain Containing (PYCARD))
    Synonyms
    ASC Peptide, CARD5 Peptide, TMS Peptide, TMS-1 Peptide, TMS1 Peptide, 9130417A21Rik Peptide, Asc Peptide, TNS1 Peptide, masc Peptide, asc1 Peptide, zAsc Peptide, zgc:109874 Peptide, PYD and CARD domain containing Peptide, PYCARD Peptide, Pycard Peptide, pycard Peptide
    Gene ID
    29108
    NCBI Accession
    NP_037390, NP_660183
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