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Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (C-Term) Peptide

Details for Product No. ABIN688560, Supplier: Log in to see
Protein Name
  • A830034N06Rik
  • AI256736
  • AI848246
  • AW546539
  • hUBA3
  • Ube1c
  • ube1c
  • UBE1C
  • wu:fb75e04
  • wu:fc37b11
  • zgc:55528
Protein Region
C-Term
Source
Synthetic
Peptide Type
Synthetic
Application
Blocking Peptide (BP)
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Specificity The synthetic peptide sequence used to generate the antibody AP1063b was selected from the C-term region of human UBE1C. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Background The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
Restrictions For Research Use only
Storage 4
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Bohnsack, Haas: "Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 26823-30, 2003

Walden, Podgorski, Schulman: "Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8." in: Nature, Vol. 422, Issue 6929, pp. 330-4, 2003

Gong, Yeh: "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 12036-42, 1999

Gubin, Njoroge, Bouffard, Miller: "Gene expression in proliferating human erythroid cells." in: Genomics, Vol. 59, Issue 2, pp. 168-77, 1999

Osaka, Kawasaki, Aida, Saeki, Chiba, Kawashima, Tanaka, Kato: "A new NEDD8-ligating system for cullin-4A." in: Genes & development, Vol. 12, Issue 15, pp. 2263-8, 1998