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Ubiquitin-Like Modifier Activating Enzyme 3 (UBA3) (C-Term) Peptide
|5 references available|
|Price||49.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Characteristics||Blocking peptide for UBE1C (C-term) antibody ABIN387909|
|Specificity||The synthetic peptide sequence used to generate the antibody AP1063b was selected from the C-term region of human UBE1C. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
Background: The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E1 ubiquitin-activating enzyme family. The encoded enzyme associates with AppBp1, an amyloid beta precursor protein binding protein, to form a heterodimer, and then the enzyme complex activates NEDD8, a ubiquitin-like protein, which regulates cell division, signaling and embryogenesis. Multiple alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles|
|Restrictions||For Research Use only|
Osaka, Kawasaki, Aida et al.: "A new NEDD8-ligating system for cullin-4A." in: Genes & development, Vol. 12, Issue 15, pp. 2263-8, 1998 (PubMed).
Gong, Yeh: "Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway." in: The Journal of biological chemistry, Vol. 274, Issue 17, pp. 12036-42, 1999 (PubMed).
Gubin, Njoroge, Bouffard et al.: "Gene expression in proliferating human erythroid cells." in: Genomics, Vol. 59, Issue 2, pp. 168-77, 1999 (PubMed).
Walden, Podgorski, Schulman: "Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8." in: Nature, Vol. 422, Issue 6929, pp. 330-4, 2003 (PubMed).
Bohnsack, Haas: "Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 26823-30, 2003 (PubMed).