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SUMO1 Activating Enzyme Subunit 2 (UBA2) (N-Term) Peptide

Details for Product No. ABIN688581, Supplier: Login to see
Protein Name
  • ATSAE2
  • EMB2764
  • EMBRYO DEFECTIVE 2764
  • F3K23.23
  • F3K23_23
  • SUMO-ACTIVATING ENZYME 2
  • SUMO-activating enzyme 2
  • ARX
  • SAE2
  • AA986091
  • Arx
  • Sae2
  • UBA1
  • Ubl1a2
  • Uble1b
  • sae2
  • sae2-B
  • uba2-a
  • uba2-b
  • uble1b-B
  • sae2b
  • uble1b
  • wu:fi17g06
  • zgc:66354
Protein Region
N-Term
Source
Synthetic
Peptide Type
Synthetic
Application
Blocking Peptide (BP)
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Immunogen Synthetic peptide
Specificity The synthetic peptide sequence used to generate the antibody AP1065a was selected from the N-term region of human UBA2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Background Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.
Restrictions For Research Use only
Storage 4
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).