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Details for Product No. ABIN688583

SUMO1 Activating Enzyme Subunit 2 (UBA2) (C-Term), (Ala600) Peptide

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Protein Name
Synonyms ARX, SAE2, Sae2, Uble1b, ATSAE2, EMB2764, EMBRYO DEFECTIVE 2764, F3K23.23, F3K23_23, SUMO-ACTIVATING ENZYME 2, SUMO-activating enzyme 2, sae2, sae2-B, uba2-a, uba2-b, uble1b-B
Protein Region
C-Term, Ala600
Application
Blocking Peptide (BP)
Pubmed 3 references available
Catalog no. ABIN688583
Quantity 0.1 mg
Price
49.50 $   Plus shipping costs $45.00
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Immunogen Synthetic peptide
Specificity The synthetic peptide sequence used to generate the antibody AP1065c was selected from the C-term region of human UBA2 . A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Alternative Name UBA2
Background Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.
Comment

Background: Ubiquitin is covalently attached to target proteins by a multienzymatic system consisting of E1 (ubiquitin-activating), E2 (ubiquitin-conjugating), and E3 (ubiquitin-ligating) enzymes. NEDD8, a ubiquitin-like protein, is conjugated to proteins in a manner analogous to ubiquitinylation. beta-amyloid precursor protein-binding protein-1 (APPBP1) can bind to NEDD8 in rabbit reticulocyte lysates. However, since APPBP1 shows similarity to only the N-terminal domain of an E1 enzyme, it must interact with a protein showing similarity to the C-terminal region of E1s. By searching sequence databases, a cDNAs encoding UBA3 was identified as the human homolog of yeast Uba3. The predicted 442-amino acid UBA3 protein shares 43% sequence identity with yeast Uba3. In vitro, UBA3 formed a complex with APPBP1 and a thioester linkage with NEDD8. APPBP1/UBA3 complex may function as an E1-like enzyme for the activation of NEDD8.

Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Okuma, Honda, Ichikawa et al.: "In vitro SUMO-1 modification requires two enzymatic steps, E1 and E2." in: Biochemical and biophysical research communications, Vol. 254, Issue 3, pp. 693-8, 1999 (PubMed).

Desterro, Rodriguez, Kemp et al.: "Identification of the enzyme required for activation of the small ubiquitin-like protein SUMO-1." in: The Journal of biological chemistry, Vol. 274, Issue 15, pp. 10618-24, 1999 (PubMed).

Gong, Li, Millas et al.: "Molecular cloning and characterization of human AOS1 and UBA2, components of the sentrin-activating enzyme complex." in: FEBS letters, Vol. 448, Issue 1, pp. 185-9, 1999 (PubMed).

Alternatives for antigen "SUMO1 Activating Enzyme Subunit 2 (UBA2)", type "Peptides"
Reactivities (1)
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