Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) Peptide

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Protein Name
  • CG10679
  • Dmel\\CG10679
  • NEDD8
  • dNEDD8
  • nedd8
  • ubiquitin
  • si:zc14a17.5
  • GB17785
  • DDBDRAFT_0218116
  • DDBDRAFT_0238041
  • DDB_0218116
  • DDB_0238041
  • NEDD-8
  • Rub1
  • CG10679 gene product from transcript CG10679-RA
  • neural precursor cell expressed, developmentally down-regulated 8
  • Nedd8
  • ubiquitin-like protein Nedd8
  • NEDD8 protein
  • NEDD8-like
  • neural precursor cell expressed, developmentally down-regulated gene 8
  • Nedd8
  • nedd8
  • NEDD8
  • LOC100520086
Protein Region
N-Term
Source
Synthetic
Peptide Type
Synthetic
Application
Blocking Peptide (BP)
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Specificity The synthetic peptide sequence used to generate the antibody AP1226a was selected from the N-term region of human NEDD8. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Background NEDD8 is a ubiquitin-like protein which plays an important role in cell cycle control and embryogenesis. Covalent attachment to its substrates requires prior activation by the E1 complex UBE1C-APPBP1 and linkage to the E2 enzyme UBE2M. Attachment of NEDD8 to cullins activates their associated E3 ubiquitin ligase activity, and thus promotes polyubiquitination and proteasomal degradation of cyclins and other regulatory proteins.
Restrictions For Research Use only
Storage 4
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Wu, Yamoah, Dolios, Gan-Erdene, Tan, Chen, Lee, Wei, Wilkinson, Wang, Pan: "DEN1 is a dual function protease capable of processing the C terminus of Nedd8 and deconjugating hyper-neddylated CUL1." in: The Journal of biological chemistry, Vol. 278, Issue 31, pp. 28882-91, 2003 (PubMed).

Bohnsack, Haas: "Conservation in the mechanism of Nedd8 activation by the human AppBp1-Uba3 heterodimer." in: The Journal of biological chemistry, Vol. 278, Issue 29, pp. 26823-30, 2003 (PubMed).

Fan, Bigsby, Nephew et al.: "The NEDD8 pathway is required for proteasome-mediated degradation of human estrogen receptor (ER)-alpha and essential for the antiproliferative activity of ICI 182,780 in ERalpha-positive breast ..." in: Molecular endocrinology (Baltimore, Md.), Vol. 17, Issue 3, pp. 356-65, 2003 (PubMed).

Walden, Podgorski, Schulman: "Insights into the ubiquitin transfer cascade from the structure of the activating enzyme for NEDD8." in: Nature, Vol. 422, Issue 6929, pp. 330-4, 2003 (PubMed).

Liu, Furukawa, Matsumoto, Xiong: "NEDD8 modification of CUL1 dissociates p120(CAND1), an inhibitor of CUL1-SKP1 binding and SCF ligases." in: Molecular cell, Vol. 10, Issue 6, pp. 1511-8, 2002 (PubMed).