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PIASx1/2 (C-Term) Peptide
|4 references available|
|Price||49.50 $ Plus shipping costs $45.00|
|Availability||Will be delivered in 2 to 3 Business Days|
|Characteristics||Blocking peptide for PIASx1/2 (C-term) antibody ABIN388059|
|Specificity||The synthetic peptide sequence used to generate the antibody AP1247a was selected from the C-term region of human PIASx1/2. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.|
Background: PIASX functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. PIASX binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.
|Storage||Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles|
|Restrictions||For Research Use only|
Liu, Liao, Rao et al.: "Inhibition of Stat1-mediated gene activation by PIAS1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 18, pp. 10626-31, 1998 (PubMed).
Wu, Cetinkaya, Munoz-Alonso et al.: "Myc represses differentiation-induced p21CIP1 expression via Miz-1-dependent interaction with the p21 core promoter." in: Oncogene, Vol. 22, Issue 3, pp. 351-60, 2003 (PubMed).
Arora, Liu, He et al.: "PIASx is a transcriptional co-repressor of signal transducer and activator of transcription 4." in: The Journal of biological chemistry, Vol. 278, Issue 24, pp. 21327-30, 2003 (PubMed).
Sakurai, Itoh, Higashitsuji et al.: "A cleaved form of MAGE-A4 binds to Miz-1 and induces apoptosis in human cells." in: The Journal of biological chemistry, Vol. 279, Issue 15, pp. 15505-14, 2004 (PubMed).