POU Class 5 Homeobox 1 (POU5F1) (Ser136) Peptide

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Protein Name
  • POU5F1L
  • POU5F1
  • OCT3
  • OCT4
  • OTF-3
  • OTF3
  • OTF4
  • Oct-3
  • Oct-4
  • NF-A3
  • Oct-3/4
  • Oct3
  • Oct3/4
  • Oct4
  • Otf-3
  • Otf-4
  • Otf3
  • Otf3-rs7
  • Otf3g
  • Otf4
  • gp9
  • gp-9
  • oct4
  • pou2
  • POU-2
  • cb197
  • spg/pou2
  • chunp6868
  • wu:fd18d06
  • POU class 5 homeobox 1
  • POU domain, class 5, transcription factor 1
  • POU domain, class 5, transcription factor 3
  • POU5F1
  • Pou5f1
  • pou5f3
Protein Region
Ser136
Source
Synthetic
Peptide Type
Synthetic
Application
Blocking Peptide (BP)
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Specificity The synthetic peptide sequence used to generate the antibody AP2046d was selected from the S136 region of human OCT3/4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Background Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.
Restrictions For Research Use only
Storage 4
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Looijenga, Stoop, de Leeuw, de Gouveia Brazao, Gillis, van Roozendaal, van Zoelen, Weber, Wolffenbuttel, van Dekken, Honecker, Bokemeyer, Perlman, Schneider, Kononen, Sauter, Oosterhuis: "POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors." in: Cancer research, Vol. 63, Issue 9, pp. 2244-50, 2003 (PubMed).

Reményi, Lins, Nissen, Reinbold, Schöler, Wilmanns: "Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers." in: Genes & development, Vol. 17, Issue 16, pp. 2048-59, 2003 (PubMed).

Crouau-Roy, Amadou, Bouissou, Clayton, Vernet, Ribouchon, Pontarotti: "Localization of the OTF3 gene within the human MHC class I region by physical and meiotic mapping." in: Genomics, Vol. 21, Issue 1, pp. 241-3, 1994 (PubMed).

Takeda, Seino, Bell: "Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues." in: Nucleic acids research, Vol. 20, Issue 17, pp. 4613-20, 1992 (PubMed).