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POU Domain, Class 5, Transcription Factor 1 (POU5F1) (Ser136) Peptide

Name

POU Domain, Class 5, Transcription Factor 1 (POU5F1)
Synonyms gp9, gp-9, oct4, pou2, POU-2, cb197, spg/pou2, chunp6868, wu:fd18d06, POU5F1, OCT3, OTF3, OTF4, Oct4
Binding Site

Ser136
4 references available
Certificates ISO 9001:2008
Catalog no. ABIN693235
Quantity 0.1 mg
Price 49.50 $   Plus shipping costs $45.00
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Immunogen Synthetic peptide
Characteristics Blocking peptide for OCT3/4 (S136) antibody ABIN388790
Specificity The synthetic peptide sequence used to generate the antibody AP2046d was selected from the S136 region of human OCT3/4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Comments

Background: Transcription factors containing the POU homeodomain have been shown to be important regulators of tissue-specific gene expression in lymphoid and pituitary differentiation and in early mammalian development. Two forms of OCT3 mRNA are expressed in adult tissues as a result of alternative splicing--OCT3A and OCT3B. Reverse transcriptase PCR showed low level of expression in both OCT3A and OCT3B mRNA in all adult human tissues examined. Oct3 is present in mouse oocytes before and after fertilization. When fertilized oocytes were injected with antisense Oct3 oligonucleotides or double-stranded DNA containing the octamer motif, embryonic DNA synthesis was inhibited and the embryos were arrested at the one-cell stage.

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Storage Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Restrictions For Research Use only

Publications
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Product Takeda, Seino, Bell: "Human Oct3 gene family: cDNA sequences, alternative splicing, gene organization, chromosomal location, and expression at low levels in adult tissues." in: Nucleic acids research, Vol. 20, Issue 17, pp. 4613-20, 1992 (PubMed).

Crouau-Roy, Amadou, Bouissou et al.: "Localization of the OTF3 gene within the human MHC class I region by physical and meiotic mapping." in: Genomics, Vol. 21, Issue 1, pp. 241-3, 1994 (PubMed).

Looijenga, Stoop, de Leeuw et al.: "POU5F1 (OCT3/4) identifies cells with pluripotent potential in human germ cell tumors." in: Cancer research, Vol. 63, Issue 9, pp. 2244-50, 2003 (PubMed).

Reményi, Lins, Nissen et al.: "Crystal structure of a POU/HMG/DNA ternary complex suggests differential assembly of Oct4 and Sox2 on two enhancers." in: Genes & development, Vol. 17, Issue 16, pp. 2048-59, 2003 (PubMed).