NFkB cRel (pSer536) Peptide

Details for Product No. ABIN694005
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Protein Name
Synonyms NFkB
Protein Region
pSer536
Application
Blocking Peptide (BP)
Pubmed 5 references available
Catalog no. ABIN694005
Quantity 0.1 mg
Price
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Immunogen Synthetic peptide
Specificity The synthetic peptide sequence used to generate the antibody AP3178a was selected from the 531-542 region of human Phospho-NFKB-pS536. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Alternative Name NFKB
Background NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).
Comment

Background: NFKB1 or NFKB2 is bound to REL, RELA, or RELB to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA or NFKBIB), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).

Restrictions For Research Use only
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Yeh, Yeh, Chuang et al.: "Suppression of MEK/ERK signaling pathway enhances cisplatin-induced NF-kappaB activation by protein phosphatase 4-mediated NF-kappaB p65 Thr dephosphorylation." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26143-8, 2004 (PubMed).

Bohuslav, Chen, Kwon et al.: "p53 induces NF-kappaB activation by an IkappaB kinase-independent mechanism involving phosphorylation of p65 by ribosomal S6 kinase 1." in: The Journal of biological chemistry, Vol. 279, Issue 25, pp. 26115-25, 2004 (PubMed).

Jang, Yoon, Shin et al.: "PIAS3 suppresses NF-kappaB-mediated transcription by interacting with the p65/RelA subunit." in: The Journal of biological chemistry, Vol. 279, Issue 23, pp. 24873-80, 2004 (PubMed).

Jeong, Radonovich, Brady et al.: "HTLV-I Tax induces a novel interaction between p65/RelA and p53 that results in inhibition of p53 transcriptional activity." in: Blood, Vol. 104, Issue 5, pp. 1490-7, 2004 (PubMed).

Zamora, Meroño, Viñas et al.: "Recruitment of NF-kappaB into mitochondria is involved in adenine nucleotide translocase 1 (ANT1)-induced apoptosis." in: The Journal of biological chemistry, Vol. 279, Issue 37, pp. 38415-23, 2004 (PubMed).

Reactivities (2)
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