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ATG4 Autophagy Related 4 Homolog B (S. Cerevisiae) (ATG4B) (Glu273) Peptide

Details for Product No. ABIN699808, Supplier: Log in to see
Protein Name
  • apg4b
  • APG4B
  • AUTL1
  • 2510009N07Rik
  • AW048066
  • Apg4b
  • Atg4bl
  • Autl1
  • Aut2b2
  • autophagy related 4B, cysteine peptidase
  • autophagy-related 4b
  • cysteine protease ATG4b
  • cysteine protease ATG4B
  • ATG4 autophagy related 4 homolog B (S. cerevisiae)
  • ATG4B
  • Atg4b
  • AT3G59950
  • Tsp_08582
  • Tsp_11599
  • atg4b
Protein Region
Glu273
Source
Synthetic
Peptide Type
Synthetic
Application
Blocking Peptide (BP)
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Specificity The synthetic peptide sequence used to generate the antibody AP1809d was selected from the E273 region of human Autophagy APG4B. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
Background Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole). APG4, a cysteine protease required for autophagy, cleaves the C-terminal part of MAP1LC3, to form the activated molecule LC3-I. LC3-I is subsequently conjugated with phosphatidylethanolamine at the C-terminal glycine to form LC3-II, a marker for autophagy via its capacity to bind to autophagosomes.
Restrictions For Research Use only
Storage 4
Storage Comment Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
Expiry Date 6 months
Background publications Tanida, Sou, Ezaki, Minematsu-Ikeguchi, Ueno, Kominami: "HsAtg4B/HsApg4B/autophagin-1 cleaves the carboxyl termini of three human Atg8 homologues and delipidates microtubule-associated protein light chain 3- and GABAA receptor-associated protein-phospholipid conjugates." in: The Journal of biological chemistry, Vol. 279, Issue 35, pp. 36268-76, 2004 (PubMed).

Mariño, Uría, Puente, Quesada, Bordallo, López-Otín: "Human autophagins, a family of cysteine proteinases potentially implicated in cell degradation by autophagy." in: The Journal of biological chemistry, Vol. 278, Issue 6, pp. 3671-8, 2003 (PubMed).