Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 22-255), (Extracellular Domain) Peptide

Details for Product No. ABIN934902, Supplier: Log in to see
Protein Name
  • OTTMUSG00000014964
  • Pin1-ps1
  • Pin1l
  • dod
  • ubl5
  • pin1b
  • pin1
  • pin1a
  • DOD
  • UBL5
  • 0610025L01Rik
  • D9Bwg1161e
  • protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1, retrogene 1
  • peptidylprolyl cis/trans isomerase, NIMA-interacting 1
  • peptidyl-prolyl cis-trans isomerase NIMA-interacting 1
  • protein (peptidyl-prolyl cis/trans isomerase) NIMA-interacting 1
  • Pin1rt1
  • pin1-b
  • pin1
  • pin1-a
  • PIN1
  • ppiD
  • PITG_11123
  • Pin1
Protein Region
AA 22-255, Extracellular Domain
Origin
Human
8
7
7
4
4
4
3
1
1
Source
Escherichia coli (E. coli)
Peptide Type
Recombinant
Biological Activity
Active
Application
SDS-PAGE (SDS)
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Sequence MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE
Characteristics Purified recombinant Human Pin 1 protein
Expression System: E.coli
Bioactivity: Specific activity is > 162 nM/min/µg, and is defined as the amount of enzyme that cleaves 1 uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher.
Purity > 95 % pure
Endotoxin Level < 1.0 EU per µg of protein (determined by LAL method)
Background Human Pin1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
Alternative Names: Rotamase Pin1 protein, UBL5 protein, PPIase protein, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein, Pin -1 protein, Pin -1, Pin 1, PPIase Pin1 protein, Pin 1 protein, PPIase Pin1 protein, Rotamase Pin1 protein, DOD protein, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, PIN1 protein, PIN1 protein, EC 5.2.1.8 protein, Pin 1, EC 5.2.1.8 protein
Molecular Weight 18.2 kDa (163 AA)
Research Area Cell Cycle, Apoptosis/Necrosis
Application Notes Pin1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
Assay Procedure
  1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
    2. Add recombinant Pin 1 protein at various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
    3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
    4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
    5. Record the increase in A410 nm for 20 minutes.
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Supplied as a liquid in 20 mM Tris-HCl buffer, pH7.5, containing 0.1 M NaCl, 0 mM DTT, and 20 % glycerol.
Preservative Dithiothreitol (DTT)
Precaution of Use This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Handling Advice Avoid repeated freeze/thaw cycles.
Storage RT/-20 °C
Storage Comment Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
Supplier Images
SDS-PAGE (SDS) image for Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 22-255), (Extracellular Domain) Peptide (ABIN934902) Figure annotation denotes ug of protein loaded and % gel used.