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Cyclophilin B (PPIB) (AA 26-216) Peptide

PPIB Reactivity: Human Host: Escherichia coli (E. coli) SDS Dithiothreitol (DTT)
Catalog No. ABIN934947
  • Target See all PPIB products
    PPIB (Cyclophilin B (PPIB))
    Peptide Type
    Recombinant
    Biological Activity
    Active
    Protein Region
    AA 26-216
    Origin
    Human
    Source
    • 6
    Escherichia coli (E. coli)
    Application
    SDS-PAGE (SDS)
    Sequence
    MLLPGPSAAD EKKKGPKVTV KVYFDLRIGD EDVGRVIFGL FGKTVPKTVD NFVALATGEK GFGYKNSKFH RVIKDFMIQG GDFTRGDGTG GKSIYGERFP DENFKLKHYG PGWVSMANAG KDTNGSQFFI TTVKTAWLDG KHVVFGKVLE GMEVVRKVES TKTDSRDKPL KDVIIADCGK IEVEKPFAIA KE
    Characteristics
    Purified recombinant Human Cyclophilin B protein
    Expression System: E.coli
    Bioactivity: Specific activity is > 170 nM/min/µg, and is defined as the amount of enzyme that cleaves 1uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
    Molecular weight on SDS-PAGE will appear higher.
    Purity
    > 95 % pure
    Endotoxin Level
    < 1.0 EU per µg of protein (determined by LAL method)
  • Application Notes
    Cyclophilin B protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
    Assay Procedure
    1. Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
      2. Add recombinant Cyclophilin B protein with various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
      3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
      4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
      5. Record the increase in A410 nm for 20 minutes
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Supplied as a liquid in 20 mM Tris, pH 8.0, containing 20 mM NaCl, 0.0 mM DTT, and 10 % glycerol.
    Preservative
    Dithiothreitol (DTT)
    Precaution of Use
    This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freeze/thaw cycles.
    Storage
    RT/-20 °C
    Storage Comment
    Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
  • Target
    PPIB (Cyclophilin B (PPIB))
    Synonyms
    cypb Peptide, scylp Peptide, cyp-s1 Peptide, PPIB Peptide, Ppib Peptide, ACYPI004891 Peptide, AA408962 Peptide, AA553318 Peptide, AI844835 Peptide, Cphn-2 Peptide, Cphn2 Peptide, CyP-20b Peptide, CypB Peptide, Scylp Peptide, CYP-S1 Peptide, CYPB Peptide, OI9 Peptide, SCYLP Peptide, cb87 Peptide, sb:cb87 Peptide, wu:fa97f08 Peptide, zgc:73214 Peptide, zgc:86796 Peptide, peptidylprolyl isomerase B Peptide, peptidylprolyl isomerase B (cyclophilin B) Peptide, peptidylprolyl isomerase B L homeolog Peptide, ppib Peptide, PPIB Peptide, Ppib Peptide, CC1G_03223 Peptide, ppib.L Peptide
    Background
    Cyclophilin B (also known as PPIB, peptidylpropyl isomerase B) is a cyclosporine-binding protein and is mainly located within the endoplasmic reticulum. It is associated with the secretory pathway and released in biological fluids. This protein can bind to cells derived from T- and B-lymphocytes, and may regulate cyclosporine A-mediated immunosuppression. Recombinant human cyclophilin B was expressed in E. coli and purified by using conventional chromatography techniques.
    Alternative Names: Cphn 2 protein, AA408962 protein, CYP S1 protein, Cyclophilin B protein, PPIB protein, MGC14109 protein, S cyclophilin., 26- 216aa protein, PPIase protein, Cphn2 protein, CyP 20b protein, SCYLP protein, AA553318 protein, AI844835 protein, Peptidyl prolyl cis trans isomerase B precursor protein, Peptidylprolyl isomerase B protein, Rotamase protein, CYPB protein, MGC2224 protein, peptidyl prolyl cis trans isomerase B protein, CYP-S1: Cyclophilin-B protein
    Molecular Weight
    21.2 kDa (192 AA)
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