Phone:
+1 877 302 8632
Fax:
+1 888 205 9894 (Toll-free)
E-Mail:
orders@antibodies-online.com

Methyl-CpG Binding Domain Protein 1 (MBD1) Peptide

MBD1 Reactivity: Human Host: Synthetic BP, WB
Catalog No. ABIN979862
  • Target See all MBD1 products
    MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))
    Origin
    Human
    Source
    • 5
    Synthetic
    Application
    Blocking Peptide (BP), Western Blotting (WB)
    Characteristics
    This is a synthetic peptide designed for use in combination with anti-MBD1 antibody (Catalog #: ARP32012_P050). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
    Purification
    Purified
  • Application Notes
    Each Investigator should determine their own optimal working dilution for specific applications.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
    Concentration
    1 mg/mL
    Buffer
    Final peptide concentration is 1 mg/mL in PBS.
    Handling Advice
    Avoid repeated freeze-thaw cycles.
    Storage
    -20 °C
    Storage Comment
    For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
  • Target
    MBD1 (Methyl-CpG Binding Domain Protein 1 (MBD1))
    Synonyms
    CXXC3 Peptide, PCM1 Peptide, RFT Peptide, Cxxc3 Peptide, CCDC11 Peptide, methyl-CpG binding domain protein 1 Peptide, MBD1 Peptide, Mbd1 Peptide
    Background
    MBD1 belongs to a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MBD1 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. Five transcript variants of the MBD1 are generated by alternative splicing resulting in protein isoforms that contain one MBD domain, two to three cysteine-rich (CXXC) domains, and some differences in the COOH terminus. All five transcript variants repress transcription from methylated promoters, in addition, variants with three CXXC domains also repress unmethylated promoter activity. MBD1 and MBD2 map very close to each other on chromosome 18q21.

    Alias Symbols: CXXC3, PCM1, RFT

    Protein Interaction Partner: ATF7IP,ATF7IP2,CHAF1A,SETDB1,ATF7IP,ATF7IP2,CBX5,CHAF1A,HDAC3,HIPK3,OASL,PIAS1,PIAS2,SETDB1,SMAD1,SMAD3,SMAD5,SMAD7,SMAD9,SUV39H1,ATF7IP,CBX4,CBX5,CHAF1A,RNF2,Rnf2,SUV39H1

    Protein Size: 549
    Molecular Weight
    60 kDa
    Gene ID
    4152
    NCBI Accession
    NM_015844, NP_056669
You are here:
Support