Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2) (Middle Region) Peptide
RFWD2
Reactivity: Mouse
Host: Synthetic
BP, WB
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- Target See all RFWD2 products
- RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))
- Protein Region
- Middle Region
- Origin
- Mouse
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Source
- Synthetic
- Application
- Blocking Peptide (BP), Western Blotting (WB)
- Sequence
- MSGLYSPVSE DSTVPQFEAP SPSHSSIIDS TEYSQPPGFS GTSQTKKQPW
- Characteristics
- This is a synthetic peptide designed for use in combination with anti-Rfwd2 Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
- Purification
- Purified
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- Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
- Concentration
- 1 mg/mL
- Buffer
- Final peptide concentration is 1 mg/mL in PBS.
- Handling Advice
- Avoid repeated freeze-thaw cycles.
- Storage
- -20 °C
- Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- Target
- RFWD2 (Ring Finger and WD Repeat Domain 2, E3 Ubiquitin Protein Ligase (RFWD2))
- Synonyms
- COP1 Peptide, RNF200 Peptide, AI316802 Peptide, C80879 Peptide, Cop1 Peptide, RGD1304773 Peptide, RFWD2 Peptide, cop1 Peptide, rnf200 Peptide, zgc:163067 Peptide, ring finger and WD repeat domain 2 Peptide, ring finger and WD repeat domain 2, E3 ubiquitin protein ligase Peptide, ring finger and WD repeat domain 2, E3 ubiquitin protein ligase L homeolog Peptide, RFWD2 Peptide, Rfwd2 Peptide, rfwd2 Peptide, rfwd2.L Peptide
- Background
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Rfwd2 is an E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It is involved in JUN ubiquitination and degradation and directly involved in p53 (TP53) ubiquitination and degradation, thereby abolishing p53-dependent transcription and apoptosis. Ubiquitinates p53 independently of MDM2 or RCHY1. Rfwd2 probably mediates E3 ubiquitin ligase activity by functioning as the essential RING domain subunit of larger E3 complexes. In contrast, it does not constitute the catalytic RING subunit in the DCX DET1-COP1 complex that negatively regulates JUN, the ubiquitin ligase activity being mediated by RBX1.
Alias Symbols: AI316802, C80879, Cop1
Protein Size: 733 - Molecular Weight
- 80 kDa
- Gene ID
- 26374
- NCBI Accession
- NM_011931, NP_036061
- UniProt
- Q9R1A8
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