anti-Chicken AKT antibody for Immunohistochemistry (Paraffin-embedded Sections)

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • CWS6
  • PKB
  • PKB-ALPHA
  • PRKBA
  • RAC
  • RAC-ALPHA
  • Akt
  • Rac
  • PKB/Akt
  • PKBalpha
  • beta-actin
  • AKT/PKB
  • AKT1
  • Akt/PKB
  • Akt1
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/dAKT
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • ACT-5
  • akt-1
  • pkb
  • v-akt
  • v-akt1
  • xAct
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • AKT serine/threonine kinase 1
  • thymoma viral proto-oncogene 1
  • actin beta
  • CG4006 gene product from transcript CG4006-RE
  • Actin 5C
  • v-akt murine thymoma viral oncogene homolog 1 S homeolog
  • v-akt murine thymoma viral oncogene homolog 1
  • K+ transporter 1
  • Serine/threonine-protein kinase akt-1
  • AKT1
  • Akt1
  • ACTB
  • Act5C
  • akt1.S
  • akt1
  • KT1
  • akt-1
Epitope
C-Term
222
131
113
66
56
51
37
36
22
22
21
21
18
16
13
12
12
12
11
11
10
10
9
9
9
8
7
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
1256
649
502
63
57
57
46
35
33
30
18
8
8
6
5
5
3
3
3
2
1
1
1
1
1
Host
Rabbit
918
384
12
4
2
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
54
42
40
38
28
25
15
15
10
10
8
6
6
6
6
6
6
6
5
5
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
1071
560
479
255
187
174
128
86
67
32
26
15
13
11
11
4
4
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN615022
$ 368.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.109145 ABIN214085 IHC (p) WB Rabbit IgG AA 121-170 Log in to see Polyclonal
1 ABIN5556658 EIA IF IHC (fro) IHC (p) IP WB Rabbit AA 460-480, C-Term Log in to see Polyclonal
1 ABIN5556659 EIA IF IHC (p) WB Rabbit AA 460-480, C-Term Log in to see Polyclonal
1 ABIN302693 EIA IHC (p) WB Rabbit pSer473 Log in to see Polyclonal
1 ABIN2615404 IHC (p) WB Rabbit IgG AA 70-90 Log in to see Polyclonal
1 ABIN609140 IF IHC (p) IP ELISA WB Rabbit AA 404-418 Log in to see Polyclonal
1 ABIN2615440 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2738221 IHC (p) WB Rabbit C-Term Log in to see Polyclonal
1 ABIN2738220 ICC IF IHC (p) WB Rabbit C-Term Log in to see Polyclonal

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope C-Term
(222), (131), (113), (66), (56), (51), (37), (36), (22), (22), (21), (21), (18), (16), (13), (12), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Human, Mouse (Murine), Rat (Rattus)
(1256), (649), (502), (63), (57), (57), (46), (35), (33), (30), (18), (8), (8), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(918), (384), (12), (4), (2)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(54), (42), (40), (38), (28), (25), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(1071), (560), (479), (255), (187), (174), (128), (86), (67), (32), (26), (15), (13), (11), (11), (4), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Sequence C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A
Specificity Recognizes AKT (AKT1/AKT2/AKT3). The sequence used to generate this antibody, has a high degree of similarity to regions found in AKT1, AKT2 and AKT3, and thus may cross react with all of these proteins.
Cross-Reactivity (Details) Species reactivity (expected):Chicken.
Species reactivity (tested):Human, Mouse and Rat.
Purification Delipidation and Defibrination
Immunogen Synthetic peptide -KLH conjugated corresponding to the C-terminus (460-480) of Human, Rat, Mouse and Chicken AKT proteins conjugated to KLH using maleimide.

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis and (ii) promotion of proliferation.Synonyms: AKT-2, AKT-3 Review 010, Akt-1, C-AKT, PKB gamma, PKBG, Protein kinase B, RAC-PK-alpha, RAC-PK-beta, RAC-PK-gamma
Gene ID 207
NCBI Accession NP_001014431
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Format Liquid
Buffer 0.02 M potassium phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.09 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Dilute only prior to immediate use.

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN615022) Immunofluorescence Microscopy: Rabbit anti-AKT antibody was used at a 1/80 dilution t...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN615022) AP22624PU-N AKT antibody staining of Formalin-Fixed Paraffin-Embedded Human Liver at ...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN615022) AP22624PU-N AKT antibody staining of Formalin-Fixed Paraffin-Embedded Sections at 1/1...
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN615022) AP22624PU-N AKT antibody staining at 1/500 dilution by Immunoblotting.