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|Application / Reactivity||Rat (Rattus)|
|Dot Blot (DB)||1 Antibodies|
|Enzyme Immunoassay (EIA)||20 Antibodies|
|Flow Cytometry (FACS)||49 Antibodies|
|Fluorescence Microscopy (FM)||2 Antibodies|
|Fluorescence Resonance Energy Transfer Microscopy (FRET)||1 Antibodies|
|Immunochromatography (IC)||5 Antibodies|
|Immunocytochemistry (ICC)||28 Antibodies|
|Immunofluorescence (IF)||107 Antibodies|
|Immunofluorescence (fixed cells) (IF/ICC)||11 Antibodies|
|Immunofluorescence (Paraffin-embedded Sections) (IF (p))||26 Antibodies|
|Immunohistochemistry (IHC)||218 Antibodies|
|Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp))||1 Antibodies|
|Immunohistochemistry (Frozen Sections) (IHC (fro))||2 Antibodies|
|Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))||90 Antibodies|
|Immunoprecipitation (IP)||58 Antibodies|
|Intracellular Staining (ICS)||3 Antibodies|
|Simple Western (SimWes)||2 Antibodies|
|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies|
|Reactivity||Chimpanzee, Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AKT antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
|4 references available|
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Product Details anti-AKT AntibodyTarget Details AKT Application Details Handling References for anti-AKT Antibody (ABIN400784) Images
|Specificity||This phospho-specific monoclonal antibody reacts with Human and Mouse AKT pSer473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Cross-reactivity with AKT2 and AKT3 has not been determined.|
Species reactivity (expected):Mouse, Chimpanzee, Rat.
Species reactivity (tested):Human.
|Characteristics||Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase|
|Purification||Protein A Chromatography|
|Immunogen||Synthetic peptide corresponding to residues surrounding Ser473 of Human AKT1 protein|
Target Details AKTProduct Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN400784) Images back to top
|Alternative Name||AKT1 / PKB (AKT1 Antibody Abstract)|
|Background||AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphory-lates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase|
|Research Area||Signaling, Protein Modifications, Kinases/Phosphatases|
|Pathways||PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN400784) Images back to top
ELISA: 1/20,000. Immunoprecipitation. Immunoflourescence: 1/500-1/3,000. Western blot: 1/500-1/3,000 (Expect a band approximately 56 kDa in size corresponding tophosphorylated AKT protein by in the appropriate cell lysate or extract). Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections: 20 μg/mL (Nopre-treatment of sample is required).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN400784) Images back to top
|Concentration||1.02 mg/mL (by UV absorbance at 280 nm)|
|Buffer||0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C/-20 °C|
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C or below forlonger. Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf life: one year from despatch.
|Expiry Date||12 months|
References for anti-AKT Antibody (ABIN400784)Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001
Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001
Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991
Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987
ImagesProduct Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN400784) back to top