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anti-Cow (Bovine) AKT antibody for Western Blotting

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Epitope
N-Term
142
114
87
85
54
39
38
38
26
25
21
20
18
16
15
15
14
13
13
11
11
10
8
7
6
6
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
1064
472
380
42
40
30
30
20
16
15
10
6
5
3
2
2
1
1
1
Host
Rabbit
877
208
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
40
33
33
32
27
25
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
879
516
414
201
109
107
107
71
42
36
24
12
11
6
3
3
3
3
1
1
1
1
1
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Catalog No. ABIN2792186
$ 289.00
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.12303223 ABIN223011 IP IHC WB Rabbit IgG Log in to see Polyclonal 2
0.12303223 ABIN2704461 IHC WB Rabbit C-Term, pSer473 Log in to see Polyclonal
0.12303223 ABIN2704459 IC IF IHC WB Rabbit C-Term, pSer473 Log in to see Polyclonal
0.12303223 ABIN2704463 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN2704465 IP IHC WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN2704462 IHC WB Rabbit C-Term Log in to see Polyclonal
0.12303223 ABIN2705425 IC IF IP WB Rabbit pSer308 Log in to see Polyclonal
0.12303223 ABIN2705426 IC IF IHC WB Rabbit pTyr315 Log in to see Polyclonal
0.12303223 ABIN2705428 IP IHC WB Rabbit Center Log in to see Polyclonal
0.12303223 ABIN2705429 IHC WB Rabbit Center Log in to see Polyclonal
0.12303223 ABIN2705430 IC IF IHC WB Rabbit Center Log in to see Polyclonal
0.12303223 ABIN2705427 IHC WB Rabbit Center Log in to see Polyclonal
0.12303223 ABIN2467767 WB Chicken AA 2-200 Log in to see Polyclonal
0.0011806369 ABIN2957853 WB Rabbit pTyr326 Log in to see Polyclonal
0.0011806369 ABIN3197868 IHC WB Rabbit C-Term, pSer450 Log in to see Polyclonal
0.0011806369 ABIN3197869 IC IF IHC WB Rabbit pSer246 Log in to see Polyclonal
0.0011806369 ABIN3197871 IHC WB Rabbit N-Term Log in to see Polyclonal
0.0011806369 ABIN3197870 IHC WB Rabbit Log in to see Polyclonal
0.0011806369 ABIN1001747 IP IHC WB Rabbit IgG Log in to see Polyclonal
0.0011806369 ABIN926917 WB Rabbit N-Term Log in to see Polyclonal

Top referenced anti-AKT antibody for Western Blotting

  • Cow (Bovine) Polyclonal AKT Primary Antibody for IP, IHC - ABIN223011 (2 Publications): Artwohl, Muth, Kosulin, de Martin, Hölzenbein, Rainer, Freudenthaler, Huttary, Schmetterer, Waldhäusl, Baumgartner-Parzer: R-(+)-alpha-lipoic acid inhibits endothelial cell apoptosis and proliferation: involvement of Akt and retinoblastoma protein/E2F-1. in American journal of physiology. Endocrinology and metabolism 2007 (PubMed)

Similar anti-AKT Antibodies

Application / Reactivity Human Mouse (Murine) Chicken Rat (Rattus) Dog (Canine) Cow (Bovine) Pig (Porcine) Xenopus laevis
Western Blotting (WB) 850 Antibodies 404 Antibodies 35 Antibodies 330 Antibodies 27 Antibodies 33 Antibodies 14 Antibodies 4 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies 1 Antibodies 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Luminex Assay (LMNX) 3 Antibodies
Intracellular Staining (ICS) 12 Antibodies 12 Antibodies 3 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope N-Term
(142), (114), (87), (85), (54), (39), (38), (38), (26), (25), (21), (20), (18), (16), (15), (15), (14), (13), (13), (11), (11), (10), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Cow (Bovine), Dog (Canine), Human, Mouse (Murine), Pig (Porcine), Rat (Rattus), Xenopus laevis
(1064), (472), (380), (42), (40), (30), (30), (20), (16), (15), (10), (6), (5), (3), (2), (2), (1), (1), (1)
Host Rabbit
(877), (208), (14), (4), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(40), (33), (33), (32), (27), (25), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Western Blotting (WB)
(879), (516), (414), (201), (109), (107), (107), (71), (42), (36), (24), (12), (11), (6), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN2792186) Images
Sequence RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF EYLKLLGKGT FGKVILVKEK
Predicted Reactivity African clawed frog : 100 %, Chicken : 100 %, Dog : 100 %, Human : 100 %, Mouse : 100 %, Rat : 100 %, Pig : 90 %, Bovine : 80 %, Goat : 80 %, Sheep : 80 %
Characteristics This is a rabbit polyclonal antibody against AKT1. It was validated on Western Blot using a cell lysate as a positive control.
Purification Affinity Purified
Immunogen The immunogen is a synthetic peptide directed towards the N terminal region of human AKT1

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN2792186) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background AKT1 is a general protein kinase capable of phosphorylating several known proteins.The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
Molecular Weight 56 kDa
Gene ID 207
NCBI Accession NM_005163, NP_005154
UniProt P31749
Research Area Cancer, Cell Cycle, Apoptosis/Necrosis, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN2792186) Images back to top
Application Notes Optimal working dilutions should be determined experimentally by the investigator.
Comment

Antigen size: 480 AA

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN2792186) Images back to top
Format Liquid
Reconstitution Add 50 µL of distilled water.
Concentration 1 mg/mL
Buffer PBS buffer with 2 % sucrose
Handling Advice Avoid repeated freeze-thaw cycles.
Storage 4 °C,-20 °C
Storage Comment For short term use, store at 2-8 °C up to 1 week. For long term storage, store at -20 °C in small aliquots to prevent freeze-thaw cycles.

References for anti-AKT Antibody (ABIN2792186)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Kim, Kim, Lee: "Expression of caveolin-1 is correlated with Akt-1 in colorectal cancer tissues." in: Experimental and molecular pathology, Vol. 80, Issue 2, pp. 165-70, 2006

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN2792186) back to top
Supplier Images
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (N-Term) (ABIN2792186) WB Suggested Anti-AKT1 Antibody
Positive Control: Lane 1:541 µg preterm baboon...