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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Dog (Canine)|
|Immunofluorescence (fixed cells) (IF/ICC)||15 Antibodies||12 Antibodies||11 Antibodies|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Simple Western (SimWes)||2 Antibodies||2 Antibodies||2 Antibodies|
|Western Blotting (WB)||887 Antibodies||530 Antibodies||412 Antibodies||42 Antibodies|
|Stimulated Emission Depletion Microscopy (STED)||1 Antibodies||1 Antibodies||1 Antibodies|
|RNA Interference (RNAi)||1 Antibodies|
|Proximity Ligation Assay (PLA)||3 Antibodies|
|Luminex Assay (LMNX)||3 Antibodies|
|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies|
|Epitope||AA 466-480 Alternatives|
|Reactivity||Dog (Canine), Human, Mouse (Murine), Rat (Rattus) Alternatives|
|Conjugate||This AKT antibody is un-conjugated Alternatives|
Enzyme Immunoassay (EIA), Western Blotting (WB)
|Supplier||Log in to see|
Product Details anti-AKT AntibodyTarget Details AKT Application Details Handling Images
|Specificity||This antibody specifically recognizes the C-terminus of protein kinase B (aa 466-480).|
|Characteristics||Synonyms: Akt-1, RAC-PK-alpha, Protein kinase B, C-AKT, RAC-alpha serine/threonine-protein kinase|
|Purification||Subsequent Thiophilic Adsorption and Size Exclusion Chromatography|
|Components||incl. positive Control|
|Immunogen||Synthetic peptide conjugated to KLH|
Target Details AKTProduct Details anti-AKT Antibody Application Details Handling Images back to top
|Alternative Name||AKT1 / PKB (AKT1 Antibody Abstract)|
|Background||The PKB (RAC/akt) protein kinases are a family of second messenger-regulated serine/threonine kinases. Three mammalian isoforms (alpha, beta, gamma) have been identified so far, the alpha-isoform being the cellular homologue of the v-akt oncogene. Stimulation of cells with growth factors leads to activation of PKB by a phosphoinositide-3-kinase dependent signal transduction pathway.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase|
|Molecular Weight||60 kDa|
|Research Area||Signaling, Protein Modifications, Kinases/Phosphatases|
|Pathways||PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-AKT Antibody Target Details AKT Handling Images back to top
ELISA: 0.05 μg/mL. Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: Casein/Tween 20 based blocking and blot incubationbuffer. Positive Control: HepG2 untreated (See Protocols).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT Antibody Target Details AKT Application Details Images back to top
|Reconstitution||Restore with 1 mL H2O (15 min, RT)|
|Buffer||1 mL2 x PBS containing 0.09 % Sodium Azide, PEG and Sucrose|
|Precaution of Use||This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||-20 °C/-80 °C|
Store lyophilized (preferably in a desiccator) at -20 °C and reconstituted (aliquote andfreeze in liquid nitrogen) at -80 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.
|Expiry Date||12 months|
ImagesProduct Details anti-AKT Antibody Target Details AKT Application Details Handling back to top