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|Application / Reactivity||Human||Mouse (Murine)||Rat (Rattus)||Chicken||Cow (Bovine)||Sheep (Ovine)||Dog (Canine)||Xenopus laevis||Monkey||Goat|
|Immunofluorescence (fixed cells) (IF/ICC)||15 Antibodies||12 Antibodies||11 Antibodies||4 Antibodies||5 Antibodies||1 Antibodies|
|Cytometry by Time of Flight (CyTOF)||1 Antibodies|
|Simple Western (SimWes)||2 Antibodies||2 Antibodies||2 Antibodies|
|Western Blotting (WB)||982 Antibodies||553 Antibodies||52 Antibodies||431 Antibodies||42 Antibodies||50 Antibodies||8 Antibodies||60 Antibodies||28 Antibodies||5 Antibodies|
|Antigen||V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies|
|Epitope||AA 121-170 Alternatives|
|Reactivity||Chicken, Cow (Bovine), Dog (Canine), Goat, Guinea Pig, Hamster, Horse (Equine), Human, Monkey, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Xenopus laevis Alternatives|
|Conjugate||This AKT antibody is un-conjugated Alternatives|
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
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Product Details anti-AKT AntibodyTarget Details AKT Application Details Handling Images
|Predicted Reactivity||Percent identity by BLAST analysis: Human, Mouse, Rat, Dog, Chicken, Xenopus (100%) Pig (93%) Sheep, Bovine, Goat (86%).|
Synthetic peptide located between aa121-170 of human AKT1 (P31749, NP_005154). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Gibbon, Monkey, Galago, Marmoset, Mouse, Rat, Hamster, Panda, Dog, Horse, Opossum, Guinea pig, Turkey, Zebra finch, Chicken, Xenopus (100%), Pig, Lizard (93%), Sheep, Goat, Bovine (86%), Baboon, Elephant, Rabbit, Salmon (85%).
Type of Immunogen: Synthetic peptide
Target Details AKTProduct Details anti-AKT Antibody Application Details Handling Images back to top
|Alternative Name||AKT1 / AKT (AKT1 Antibody Abstract)|
Name/Gene ID: AKT1
Family: Protein Kinase
Synonyms: AKT1, AKT, AKT-1, C-AKT, Pan-AKT, PKB alpha, PKBalpha, Proto-oncogene c-Akt, Protein kinase B, Rac protein kinase alpha, PRKBA, Protein kinase B alpha, RAC-PK-alpha, PKB, PKB-ALPHA, RAC, RAC-ALPHA
|Pathways||PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals|
Application DetailsProduct Details anti-AKT Antibody Target Details AKT Handling Images back to top
Approved: IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 5 μg/mL.
|Restrictions||For Research Use only|
HandlingProduct Details anti-AKT Antibody Target Details AKT Application Details Images back to top
|Buffer||Lyophilized from PBS with 2 % sucrose|
|Handling Advice||Avoid repeat freeze-thaw cycles.|
|Storage||4 °C,-20 °C|
Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than one week): +4°C. Avoid repeat freeze-thaw cycles.
ImagesProduct Details anti-AKT Antibody Target Details AKT Application Details Handling back to top