You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Human AKT antibody for Enzyme Immunoassay

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Epitope
AA 460-480, C-Term
142
113
88
85
54
39
38
38
26
25
21
20
18
16
15
15
14
13
13
11
11
10
8
6
6
6
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Chicken, Human, Mouse (Murine), Rat (Rattus)
1064
472
380
42
41
31
30
20
17
15
10
6
5
4
2
2
1
1
1
Host
Rabbit
877
208
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
40
33
33
32
27
25
5
4
4
2
2
2
2
2
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
879
516
414
200
108
107
106
71
42
35
24
12
11
5
3
3
3
3
1
1
1
1
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN117897
$ 500.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.0008833851 ABIN400785 EIA IF IHC (p) IP WB Mouse IgG1 Ser473 Log in to see 17F6-B11 4
0.0008833851 ABIN400784 EIA IF IHC (p) IP WB Mouse IgG1 Ser473 Log in to see 17F6-B11 4
0.0008833851 ABIN400791 EIA IHC (fro) IP WB Mouse IgG1 C-Term Log in to see 18F10-E5 4
0.0008833851 ABIN401485 EIA WB Sheep IgG Internal Region Log in to see Polyclonal 7
0.0008833851 ABIN400786 EIA IF IHC (p) IP WB Mouse IgG1 Thr308 Log in to see 18F3-H11 4
0.0008833851 ABIN358371 DB EIA Rabbit Ig pSer473 Log in to see Polyclonal 5
0.0008833851 ABIN358075 EIA IHC (p) WB Rabbit Ig pSer129 Log in to see Polyclonal 4
0.0008833851 ABIN358076 EIA WB Rabbit Ig pTyr326 Log in to see Polyclonal 4
0.0008833851 ABIN358077 EIA WB Rabbit Ig pTyr326 Log in to see Polyclonal 4
0.0008833851 ABIN359075 EIA FACS WB Rabbit Ig C-Term, AA 446-475 Log in to see Polyclonal 1
0.0008833851 ABIN950327 EIA IHC (p) WB Rabbit Ig AA 289-318 Log in to see Polyclonal 4
0.0008833851 ABIN950329 EIA FACS IF IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 4
0.0008833851 ABIN401484 EIA WB Sheep IgG Internal Region Log in to see Polyclonal 7
0.0008833851 ABIN117898 EIA IF IHC (p) WB Rabbit AA 460-480, C-Term Log in to see Polyclonal 2
0.0008833851 ABIN499261 EIA IHC (p) WB Rabbit IgG Log in to see Polyclonal 4
0.0008833851 ABIN400787 EIA IF IHC (p) IP WB Mouse IgG1 Thr308 Log in to see 18F3-H11
0.0008833851 ABIN265304 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008833851 ABIN265302 EIA IHC (p) WB Rabbit Log in to see Polyclonal
0.0008833851 ABIN126853 EIA WB Mouse IgG1 pSer473 Log in to see 1-10E-007
0.0008833851 ABIN126858 EIA WB Mouse IgG1 AA 466-480 Log in to see 5C10

Top referenced anti-AKT antibody for Enzyme Immunoassay

Similar anti-AKT Antibodies

Application / Reactivity Rat (Rattus) Mouse (Murine)
Dot Blot (DB) 1 Antibodies 1 Antibodies
ELISA 144 Antibodies
Enzyme Immunoassay (EIA) 21 Antibodies
Flow Cytometry (FACS) 10 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 18 Antibodies
Immunofluorescence (IF) 84 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 205 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 3 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 48 Antibodies
Immunoprecipitation (IP) 39 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies
Western Blotting (WB) 330 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 460-480, C-Term
(142), (113), (88), (85), (54), (39), (38), (38), (26), (25), (21), (20), (18), (16), (15), (15), (14), (13), (13), (11), (11), (10), (8), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Chicken, Human, Mouse (Murine), Rat (Rattus)
(1064), (472), (380), (42), (41), (31), (30), (20), (17), (15), (10), (6), (5), (4), (2), (2), (1), (1), (1)
Host Rabbit
(877), (208), (14), (4), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(40), (33), (33), (32), (27), (25), (5), (4), (4), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
(879), (516), (414), (200), (108), (107), (106), (71), (42), (35), (24), (12), (11), (5), (3), (3), (3), (3), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN117897) Images
Sequence C-R-PH-F-P-Q-F-S-Y-S-A-S-G-T-A
Specificity This antiserum reacts with the AKT.
Cross-Reactivity (Details) Species reactivity (expected):Rat, Mouse and Chicken.
Species reactivity (tested):Human.
Purification Serum
Immunogen Synthetic peptide corresponding to the C-terminus (460-480) of Human, Rat and Mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN117897) Images back to top
Antigen
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background AKT is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Gene ID 207
NCBI Accession NP_001014431
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN117897) Images back to top
Application Notes ELISA: 1/2,000-1/10,000. Immunoprecipitation. Transfected/Primary cell culture. Western Blot (1/500-1/2,000): Detect a single band of the expected apparent molecularweight. Immunofluorescence using paraformaldehyde-fixed primary cardiomyocyte cultures(1/100-1/1,000). Immunohistochemistry on Formalin-Fixed Paraffin-Embedded Sections (1/500-1/2,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN117897) Images back to top
Format Liquid
Concentration 85 mg/mL (by refractometry)
Buffer 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 containing 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-AKT Antibody (ABIN117897)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN117897) back to top
Supplier Images
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 460-480) (ABIN117897) Immunofluorescence microscopy: Rabbit anti-AKT was used at a 1:80 dilution to stain c...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 460-480) (ABIN117897) Immunoblotting: Rabbit anti-AKT was used at a 1:500 dilution to detect AKT by Western...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 460-480) (ABIN117897) Immunohistochemistry. Rabbit anti-AKT was used at a 1:1,000 dilution to detect AKT by...