anti-Human AKT antibody for Enzyme Immunoassay

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
pSer129
208
123
103
65
57
40
39
30
27
22
21
21
18
18
17
14
13
12
12
11
11
10
10
9
9
9
8
7
7
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1221
647
503
63
58
56
46
35
33
30
18
8
8
6
5
5
3
3
3
2
1
1
1
1
1
Host
Rabbit
883
381
12
4
2
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
54
41
39
38
28
25
15
15
10
10
8
6
6
6
6
6
6
6
5
5
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
1040
552
455
253
183
174
120
82
67
32
26
15
13
11
11
4
3
3
3
2
1
1
1
1
1
1
1
1
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Catalog No. ABIN358075
$ 390.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN358076 EIA WB Rabbit Ig pTyr326 Log in to see Polyclonal 4
1 ABIN359075 EIA FACS WB Rabbit Ig C-Term, AA 446-475 Log in to see Polyclonal 1
1 ABIN358077 EIA WB Rabbit Ig pTyr326 Log in to see Polyclonal 4
1 ABIN950327 EIA IHC (p) WB Rabbit Ig AA 289-318 Log in to see Polyclonal 4
1 ABIN950329 EIA FACS IF IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 4
1 ABIN5556658 EIA IF IHC (fro) IHC (p) IP WB Rabbit AA 460-480, C-Term Log in to see Polyclonal
1 ABIN265302 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN265304 EIA IHC (p) WB Rabbit Log in to see Polyclonal
1 ABIN5550837 EIA IF IHC (p) IP WB Mouse IgG1 Ser473 Log in to see 17F6-B11
1 ABIN5551855 EIA WB Sheep IgG Internal Region Log in to see Polyclonal
1 ABIN5552139 DB EIA Rabbit Ig Fraction pSer473 Log in to see Polyclonal
1 ABIN126858 EIA WB Mouse IgG1 AA 466-480 Log in to see 5C10
1 ABIN126853 EIA WB Mouse IgG1 pSer473 Log in to see 1-10E-007
1 ABIN5556660 EIA WB Rabbit AA 302-311, pThr308 Log in to see Polyclonal
1 ABIN5556659 EIA IF IHC (p) WB Rabbit AA 460-480, C-Term Log in to see Polyclonal
1 ABIN5550838 EIA IF IHC (p) IP WB Mouse IgG1 Thr308 Log in to see 18F3-H11
1 ABIN126852 EIA WB Biotin Mouse IgG1 pSer473 Log in to see 1-10E-007
1 ABIN126855 EIA IHC (fro) WB Mouse IgG1 AA 107-122 Log in to see 5G12
1 ABIN453509 EIA FACS IF WB Rabbit N-Term Log in to see Polyclonal
1 ABIN359291 EIA IHC (p) WB Rabbit Ig N-Term Log in to see Polyclonal

Top referenced anti-AKT antibody for Enzyme Immunoassay

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ELISA 513 Antibodies
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FLISA 1 Antibodies
Flow Cytometry (FACS) 178 Antibodies
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Immunochromatography (IC) 11 Antibodies
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Immunofluorescence (IF) 250 Antibodies
Immunofluorescence (Cultured Cells) (IF (cc)) 1 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 418 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pSer129
(208), (123), (103), (65), (57), (40), (39), (30), (27), (22), (21), (21), (18), (18), (17), (14), (13), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1221), (647), (503), (63), (58), (56), (46), (35), (33), (30), (18), (8), (8), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(883), (381), (12), (4), (2), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(54), (41), (39), (38), (28), (25), (15), (15), (10), (10), (8), (6), (6), (6), (6), (6), (6), (6), (5), (5), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(1040), (552), (455), (253), (183), (174), (120), (82), (67), (32), (26), (15), (13), (11), (11), (4), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1), (1)
Pubmed 4 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN358075) Images
Specificity This antibody detects AKT1/PKB pSer129. Predicted to cross react with Mouse (100 % Antigen Homology).
Purification Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
Immunogen This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S129 of human AKT1.
Isotype Ig

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN358075) Images back to top
Antigen
Alternative Name AKT1 / PKB (AKT1 Antibody Abstract)
Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery.Synonyms: Akt-1, C-AKT, Protein kinase B, RAC-PK-alpha, RAC-alpha serine/threonine-protein kinase
Molecular Weight 55686 Da
Gene ID 207, 9606
UniProt P31749
Research Area Signaling, Protein Modifications, Kinases/Phosphatases
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling ProductDetails: References for anti-AKT antibody (ABIN358075) Images back to top
Application Notes ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details ProductDetails: References for anti-AKT antibody (ABIN358075) Images back to top
Format Liquid
Concentration 0.25 mg/mL
Buffer PBS with 0.09 % (W/V) Sodium Azide as preservative.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

ProductDetails: References for anti-AKT antibody (ABIN358075)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Dephoure, Zhou, Villén, Beausoleil, Bakalarski, Elledge, Gygi: "A quantitative atlas of mitotic phosphorylation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 105, Issue 31, pp. 10762-7, 2008

Background publications

McCulloch, Omens: "Myocyte shearing, myocardial sheets, and microtubules." in: Circulation research, Vol. 98, Issue 1, pp. 1-3, 2006

Margolskee: "Molecular mechanisms of bitter and sweet taste transduction." in: The Journal of biological chemistry, Vol. 277, Issue 1, pp. 1-4, 2002

Bellacosa, Chan, Ahmed, Datta, Malstrom, Stokoe, McCormick, Feng, Tsichlis: "Akt activation by growth factors is a multiple-step process: the role of the PH domain." in: Oncogene, Vol. 17, Issue 3, pp. 313-25, 1998

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling ProductDetails: References for anti-AKT antibody (ABIN358075) back to top
Supplier Images
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer129) (ABIN358075) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer129) antibody
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer129) (ABIN358075) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer129) antibody (Image 2)