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anti-Human AKT antibody for ELISA

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Reactivity
Human
1100
616
476
61
58
55
46
35
33
30
18
8
8
5
5
5
3
3
2
2
1
1
1
1
Host
Mouse
842
301
11
4
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
46
38
35
35
26
25
10
10
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
942
516
431
218
146
140
115
67
52
32
26
15
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
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Available images

Catalog No. ABIN4278989
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
10.904965 ABIN559828 ELISA WB Mouse IgG2a kappa AA 1-480, full length Log in to see 6C8
10.904965 ABIN559835 IF ELISA WB Mouse IgG2a lambda AA 1-480, full length Log in to see 6F11
10.904965 ABIN559829 IF IHC (p) IP ELISA WB Mouse IgG2a kappa AA 1-480, full length Log in to see 2E11
10.904965 ABIN559827 IP PLA RNAi ELISA Mouse IgG2a kappa AA 381-480, partial Log in to see 4C3
10.904965 ABIN559830 IHC (p) ELISA WB Mouse IgG2a kappa AA 1-480, full length Log in to see 6D6
10.904965 ABIN559831 IHC (p) ELISA WB Mouse IgG2a kappa AA 1-480, full length Log in to see 6G6
10.904965 ABIN559833 IHC (p) ELISA WB Mouse IgG2a kappa AA 1-480, full length Log in to see 4B9
10.904965 ABIN559832 IHC (p) ELISA WB Mouse IgG2a kappa AA 1-480, full length Log in to see 2H1
10.904965 ABIN559834 IF ELISA WB Mouse IgG2a AA 1-480, full length Log in to see 1D4
1 ABIN349614 IHC ELISA WB Mouse IgG1 pSer473 Log in to see 17F6-B11 5
1 ABIN1531520 ELISA WB Rabbit IgG pTyr326 Log in to see Polyclonal 2
1 ABIN1531420 ELISA WB Rabbit IgG pSer129 Log in to see Polyclonal 1
1 ABIN1043756 FM FRET STED ELISA WB Atto 594 Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
1 ABIN1043752 IP IHC ELISA WB Biotin Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
1 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region Log in to see 18F3-H11 4
1 ABIN1532434 IHC ELISA Rabbit IgG Log in to see Polyclonal 1
1 ABIN1533695 ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1532436 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1043730 IP IHC ELISA WB Mouse IgG1 kappa C-Term Log in to see 18F10-E5 4
1 ABIN1043729 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 4

Top referenced anti-AKT antibody for ELISA

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General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Reactivity Human
(1100), (616), (476), (61), (58), (55), (46), (35), (33), (30), (18), (8), (8), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(842), (301), (11), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(46), (38), (35), (35), (26), (25), (10), (10), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunohistochemistry (IHC), Western Blotting (WB)
(942), (516), (431), (218), (146), (140), (115), (67), (52), (32), (26), (15), (11), (11), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Purification Protein A purified
Immunogen Anti-AKT1 Antibody was produced in mice by repeated immunizations with a synthetic peptide corresponding to internal residues of human AKT1 protein.
Clone 5E5-F5-D7
Isotype IgG2b kappa

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name AKT (AKT1 Antibody Abstract)
Background Gene Symbol: AKT1
Gene ID 207
Research Area Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes Western Blot 1:500-1:2000, Flow Cytometry, ELISA 1:2000 - 1:10000, Immunohistochemistry 20 μg/mLAnti-AKT1 Antibody is suitable for ELISA, immunohistochemistry, and western blotting. Expect a band approximately 56 kDa in size corresponding to AKT1 protein by western blotting in the appropriate cell lysate or extract. This monoclonal antibody reacts with human AKT. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry we recommend the use of fresh frozen tissues. Attempts at staining paraffin-embedded formalin fixed tissues were negative. No pre-treatment of sample is required.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C,-20 °C
Storage Comment Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling back to top
Supplier Images
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (ABIN4278989) Western Blot: AKT Antibody (5E5.F5.D7) [NBP2-44110] - lane 1: GST-AKT1. Lane 2: GST-A...
ELISA image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (ABIN4278989) ELISA: AKT Antibody (5E5.F5.D7) [NBP2-44110] - ELISA of Mouse Monoclonal anti-AKT1 An...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (ABIN4278989) Western Blot: AKT Antibody (5E5.F5.D7) [NBP2-44110] - lane 1: LnCap lysate Lane 2: Ju...
Flow Cytometry (FACS) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (ABIN4278989) Flow Cytometry: AKT Antibody (5E5.F5.D7) [NBP2-44110] - Cells: LNCap Cells. Stimulati...