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anti-Human AKT antibody for Flow Cytometry

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
AA 282-309, Thr308
192
136
90
86
55
40
38
36
27
25
21
21
21
21
16
15
15
14
13
12
11
11
10
9
7
6
6
6
5
5
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1182
552
418
44
42
41
32
27
20
18
18
6
5
4
2
2
1
1
1
Host
Rabbit
909
317
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
46
39
35
35
26
25
10
10
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
976
555
478
225
142
133
119
71
63
36
26
11
11
5
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN654500
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.103481 ABIN359075 EIA FACS WB Rabbit Ig C-Term, AA 446-475 Log in to see Polyclonal 1
13.103481 ABIN4899444 FACS ICC SimWes WB Mouse IgG2b AA 2-480 Log in to see 281046 4
13.103481 ABIN4899445 FACS ICC Mouse IgG1 AA 2-480 Log in to see 302407 1
13.103481 ABIN4900619 FACS IHC SimWes WB Rabbit IgG pSer473 Log in to see Polyclonal 12
13.103481 ABIN2159031 FACS IF IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
13.103481 ABIN4278999 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
13.103481 ABIN4896123 FACS Allophycocyanin Mouse IgG2b AA 2-480 Log in to see 281046
13.103481 ABIN4896124 FACS PE Mouse IgG2b AA 2-480 Log in to see 281046
13.103481 ABIN4896125 FACS PE Mouse IgG1 AA 2-480 Log in to see 302407
13.103481 ABIN2159029 FACS WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
13.103481 ABIN2159030 FACS IF IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
13.103481 ABIN4278989 ELISA FACS IHC WB Mouse IgG2b kappa Log in to see 5E5-F5-D7
13.103481 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6
13.103481 ABIN4898083 FACS Alexa Fluor 488 Mouse IgG1 pSer473 Log in to see 545007
13.103481 ABIN4898084 FACS PE Mouse IgG1 pSer473 Log in to see 545007
13.103481 ABIN3029467 FACS IF ELISA WB Rabbit Ig Fraction AA 49-76 Log in to see Polyclonal
13.103481 ABIN3029457 FACS IHC ELISA WB Rabbit Ig Fraction AA 282-309 Log in to see Polyclonal
13.103481 ABIN3029468 FACS ELISA WB Rabbit Ig Fraction AA 438-468 Log in to see Polyclonal
13.103481 ABIN3029453 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 428-457 Log in to see Polyclonal
13.103481 ABIN3029459 FACS IF IHC ELISA WB Rabbit Ig Fraction AA 115-144 Log in to see Polyclonal

Top referenced anti-AKT antibody for Flow Cytometry

Similar anti-AKT Antibodies

Application / Reactivity Human
Blocking Reagent (BR) 1 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 71 Antibodies
ELISA 517 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 36 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 138 Antibodies
Fluorescence Microscopy (FM) 3 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 63 Antibodies
Immunofluorescence (IF) 223 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 441 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 282-309, Thr308
(192), (136), (90), (86), (55), (40), (38), (36), (27), (25), (21), (21), (21), (21), (16), (15), (15), (14), (13), (12), (11), (11), (10), (9), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1182), (552), (418), (44), (42), (41), (32), (27), (20), (18), (18), (6), (5), (4), (2), (2), (1), (1), (1)
Host Rabbit
(909), (317), (14), (4), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This AKT antibody is un-conjugated
(46), (39), (35), (35), (26), (25), (10), (10), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(976), (555), (478), (225), (142), (133), (119), (71), (63), (36), (26), (11), (11), (5), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN654500) Images
Specificity This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 289-318 amino acids from human AKT1.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus),Xenopus laevis
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-309 AA from human AKT1.
Clone RB27685
Isotype Ig

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN654500) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
Synonyms: AKT1,RAC-alpha serine/threonine-protein kinase,PKB, RAC
Molecular Weight 55686 DA
Gene ID 207
NCBI Accession NP_001014431, NP_001014432, NP_005154
UniProt P31749
Research Area Translation Factors, Protein Modifications, Signaling, Neurology, Cell Structure, Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cancer, Chromatin, Metabolism
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN654500) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN654500) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-AKT Antibody (ABIN654500)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Chen, Gomes, Monteiro, Wong, Wu, Ng, Karadedou, Millour, Ip, Cheung, Sunters, Chan, Lam, Khoo: "Constitutively nuclear FOXO3a localization predicts poor survival and promotes Akt phosphorylation in breast cancer." in: PLoS ONE, Vol. 5, Issue 8, pp. e12293, 2010

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN654500) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 282-309) (ABIN654500) AKT1 Antibody (Thr308) (ABIN654500) flow cytometric analysis of MDA-MB435 cells (righ...