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anti-Human AKT antibody for Flow Cytometry

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • Akt
  • akt
  • AKT
  • akt-1
  • Akt/PKB
  • AKT/PKB
  • AKT1
  • akt1
  • Akt1
  • ATAKT1
  • CG4006
  • CWS6
  • D-Akt
  • dAKT
  • dakt
  • dAkt
  • Dakt
  • DAkt
  • dAKT/dPKB
  • dAkt/PKB
  • dakt1
  • dAkt1
  • dAKT1
  • DAkt1
  • DAKT1
  • Dakt1
  • DAKT1/PKB
  • Dmel\\CG4006
  • Dpkb
  • DPKB
  • dPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • pkb
  • PKB
  • PKB-ALPHA
  • PKB/Akt
  • PKB/AKT
  • PKB/dAKT
  • PKBalpha
  • POTASSIUM TRANSPORTER
  • PRKBA
  • Rac
  • RAC
  • RAC-ALPHA
  • RacPK
  • v-akt
  • v-akt1
  • xAct
Epitope
AA 282-309, Thr308
187
136
92
88
54
38
38
38
26
25
21
20
18
16
16
15
15
14
13
13
11
11
10
8
7
6
6
5
5
5
5
4
4
4
4
4
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1142
532
398
43
41
34
33
32
20
17
10
6
5
4
2
2
2
1
1
1
Host
Rabbit
891
291
14
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
45
36
35
34
27
25
10
9
5
5
5
4
4
3
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
958
548
433
216
129
120
112
71
57
36
24
12
11
6
3
3
3
3
1
1
1
1
1
1
Supplier
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Catalog No. ABIN654500
$ 324.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
0.30226976 ABIN359075 EIA FACS WB Rabbit Ig C-Term, AA 446-475 Log in to see Polyclonal 1
0.30226976 ABIN2159031 FACS IF IHC WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.30226976 ABIN2159029 FACS WB Rabbit Ig Fraction C-Term Log in to see Polyclonal
0.30226976 ABIN2159030 FACS IF IHC WB Rabbit Ig Fraction N-Term Log in to see Polyclonal
0.30226976 ABIN2677075 FACS ICS APC Mouse IgG2a kappa pSer473 Log in to see SDRNR
0.0010482287 ABIN950329 EIA FACS IF IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 4
0.0010482287 ABIN652283 FACS WB Rabbit Ig AA 438-468, C-Term Log in to see Polyclonal 4
0.0010482287 ABIN655915 FACS IF IHC (p) WB Rabbit Ig AA 428-457, C-Term Log in to see Polyclonal 1
0.0010482287 ABIN2455512 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
0.0010482287 ABIN1981392 FACS IF IHC WB Rabbit IgG AA 428-457, C-Term Log in to see Polyclonal
0.0010482287 ABIN1981394 FACS IF IHC WB Rabbit IgG AA 115-144, N-Term Log in to see Polyclonal
0.0010482287 ABIN1981393 FACS WB Rabbit IgG AA 438-468, C-Term Log in to see Polyclonal
0.0010482287 ABIN1981391 FACS IHC WB Rabbit IgG AA 282-309, Thr308 Log in to see Polyclonal
0.0010482287 ABIN453509 EIA FACS IF WB Rabbit N-Term Log in to see Polyclonal
0.0010482287 ABIN2455513 FACS IF IHC WB Mouse IgG1 Log in to see 4C11
0.0010482287 ABIN2455515 FACS IF IHC WB Mouse IgG1 Log in to see 1G5
0.0010482287 ABIN391212 FACS IF IHC (p) WB Rabbit Ig AA 115-144, N-Term Log in to see Polyclonal
0.0010482287 ABIN652291 FACS IF WB Rabbit Ig AA 49-76, N-Term Log in to see Polyclonal
0.0010482287 ABIN2455510 FACS IF IHC WB Mouse IgG1 Log in to see 4G4
0.0010482287 ABIN2455511 FACS IF IHC WB Mouse IgG1 Log in to see 3B4

Top referenced anti-AKT antibody for Flow Cytometry

Similar anti-AKT Antibodies

Application / Reactivity Human
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Dot Blot (DB) 71 Antibodies
ELISA 512 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 36 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 125 Antibodies
Fluorescence Microscopy (FM) 3 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 59 Antibodies
Immunofluorescence (IF) 216 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 414 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 7 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 122 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 282-309, Thr308
(187), (136), (92), (88), (54), (38), (38), (38), (26), (25), (21), (20), (18), (16), (16), (15), (15), (14), (13), (13), (11), (11), (10), (8), (7), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1142), (532), (398), (43), (41), (34), (33), (32), (20), (17), (10), (6), (5), (4), (2), (2), (2), (1), (1), (1)
Host Rabbit
(891), (291), (14), (4), (1)
Clonality (Clone)
Polyclonal   ( )
Conjugate This AKT antibody is un-conjugated
(45), (36), (35), (34), (27), (25), (10), (9), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(958), (548), (433), (216), (129), (120), (112), (71), (57), (36), (24), (12), (11), (6), (3), (3), (3), (3), (1), (1), (1), (1), (1), (1)
Pubmed 1 reference available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN654500) Images
Specificity This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 289-318 amino acids from human AKT1.
Predicted Reactivity Cow (Bovine),Mouse (Murine),Rat (Rattus),Xenopus laevis
Purification This antibody is purified through a protein A column, followed by peptide affinity purification.
Immunogen This AKT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 282-309 AA from human AKT1.
Clone RB27685
Isotype Ig

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN654500) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background The serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene. [provided by RefSeq].
Synonyms: AKT1,RAC-alpha serine/threonine-protein kinase,PKB, RAC
Molecular Weight 55686 DA
Gene ID 207
NCBI Accession NP_001014431, NP_001014432, NP_005154
UniProt P31749
Research Area Translation Factors, Protein Modifications, Signaling, Neurology, Cell Structure, Ubiquitin-related antibodies, Apoptosis/Necrosis, Phospho-specific antibodies, Cell Signaling, Cancer, Chromatin, Metabolism
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN654500) Images back to top
Application Notes WB = 1:1000, IHC (p) = 1:50-100, FACS = 1:10-50
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN654500) Images back to top
Format Liquid
Concentration 0.5 mg/mL
Buffer PBS with 0.09 % (W/V) sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Maintain refrigerated at 2-8 °C for up to 6 months. For long term storage store at -20 °C in small aliquots to prevent freeze-thaw cycles.
Expiry Date 6 months

References for anti-AKT Antibody (ABIN654500)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Chen, Gomes, Monteiro, Wong, Wu, Ng, Karadedou, Millour, Ip, Cheung, Sunters, Chan, Lam, Khoo: "Constitutively nuclear FOXO3a localization predicts poor survival and promotes Akt phosphorylation in breast cancer." in: PLoS ONE, Vol. 5, Issue 8, pp. e12293, 2010

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN654500) back to top
Supplier Images
Flow Cytometry (FACS) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 282-309) (ABIN654500) AKT1 Antibody (Thr308) (ABIN654500) flow cytometric analysis of MDA-MB435 cells (righ...