anti-Human AKT antibody for Fluorescence Microscopy

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
C-Term
225
130
115
66
57
51
39
37
36
32
22
22
18
16
13
12
12
12
11
11
10
10
9
9
9
8
7
7
7
6
6
6
6
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus), Chicken
1286
669
520
63
57
56
46
35
33
30
18
8
8
6
5
5
3
3
3
2
1
1
1
1
1
Host
Rabbit
925
407
12
4
2
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
54
44
40
38
29
25
18
17
12
12
8
8
7
7
6
6
6
6
6
6
5
4
4
2
2
2
2
2
2
2
2
1
1
1
1
Application
Fluorescence Microscopy (FM), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
1080
561
484
259
209
175
128
87
67
33
26
15
12
11
11
7
4
4
3
3
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

Submit your validation data for this product and get a full refund. I want to validate this product

Learn more

Available images

Catalog No. ABIN1043683
$ 135.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1043756 FM FRET STED ELISA WB Atto 594 Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
1 ABIN5596660 ELISA FACS FM IHC IP WB Biotin Rabbit IgG Log in to see Polyclonal
1 ABIN94783 FM IHC (fp) IP ELISA WB Rabbit C-Term Log in to see Polyclonal
1 ABIN5596665 ELISA FACS FM IHC WB FITC Mouse IgG2a Internal Region Log in to see 14E5-A2-B2-H9
1 ABIN5596663 ELISA FACS FM IHC WB Biotin Mouse IgG2a Internal Region Log in to see 14E5-A2-B2-H9
1 ABIN5596664 ELISA FACS FM IHC WB Biotin Mouse IgG2a Internal Region Log in to see 5E5-F5-D7
1 ABIN5596666 ELISA FACS FM IHC WB FITC Mouse IgG2a Internal Region Log in to see 5E5-F5-D7
1 ABIN5596667 ELISA FACS FM IHC WB PE Mouse IgG2a Internal Region Log in to see 14E5-A2-B2-H9
1 ABIN5596661 ELISA FACS FM IHC WB APC Mouse IgG2a Internal Region Log in to see 14E5-A2-B2-H9
1 ABIN5596662 ELISA FACS FM IHC WB APC Mouse IgG2a Internal Region Log in to see 5E5-F5-D7
1 ABIN5596668 ELISA FACS FM IHC WB PE Mouse IgG2a Internal Region Log in to see 5E5-F5-D7
1 ABIN5596659 ELISA FACS FM IHC WB Rabbit IgG Log in to see Polyclonal

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope C-Term
(225), (130), (115), (66), (57), (51), (39), (37), (36), (32), (22), (22), (18), (16), (13), (12), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (7), (7), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus), Chicken
(1286), (669), (520), (63), (57), (56), (46), (35), (33), (30), (18), (8), (8), (6), (5), (5), (3), (3), (3), (2), (1), (1), (1), (1), (1)
Host Rabbit
(925), (407), (12), (4), (2)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(54), (44), (40), (38), (29), (25), (18), (17), (12), (12), (8), (8), (7), (7), (6), (6), (6), (6), (6), (6), (5), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Fluorescence Microscopy (FM), Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), ELISA, Western Blotting (WB)
(1080), (561), (484), (259), (209), (175), (128), (87), (67), (33), (26), (15), (12), (11), (11), (7), (4), (4), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN1043683) Images
Specificity AKT Antibody detects AKT which is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis, (ii) promotion of proliferation. Anti-AKT Antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
Characteristics AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Sterility Sterile filtered
Immunogen AKT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a synthetic peptide C-R-P-H-F-P-Q-F-S-Y-S-A-S-G-T-A corresponding to the C-terminus (460-480) of human, rat and mouse and chicken AKT proteins conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling.
Immunogen Type: Peptide

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT antibody (ABIN1043683) Images back to top
Antigen
Alternative Name AKT (AKT1 Antibody Abstract)
Background AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Synonyms: RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
Gene ID 207, 62241011
UniProt P31749
Research Area Signaling
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Signaling of Hepatocyte Growth Factor Receptor, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals, VEGF Signaling

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT antibody (ABIN1043683) Images back to top
Application Notes AKT Antibody is suitable for western blotting to detect a single band of the expected apparent molecular weight, immunohistochemistry (Formalin-fixed paraffin-embedded sections), immunofluorescence microscopy using paraformaldehyde-fixed primary cardiomyocyte cultures, immunoprecip-itation, transfected cell culture, primary cell culture and ELISA. Researchers should determine optimal titers for applications that are not stated below.
Comment

Gene Name: AKT1

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT antibody (ABIN1043683) Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity. Expiration date is one (1) year from date of opening.
Expiry Date 12 months

References for anti-AKT antibody (ABIN1043683)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Matsuda, Takano, Kageyama, Hatakeyama, Shakunaga, Kitajima, Yamazaki, Hattori: "Silencing of caspase-8 and caspase-3 by RNA interference prevents vascular endothelial cell injury in mice with endotoxic shock." in: Cardiovascular research, Vol. 76, Issue 1, pp. 132-40, 2007

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT antibody (ABIN1043683) back to top
Supplier Images
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN1043683) Immunofluorescence Microscopy of Rabbit Anti-AKT Antibody. Tissue: neonatal rat cardi...
Immunohistochemistry (IHC) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN1043683) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (Image 2)
Immunofluorescence (IF) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN1043683) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (Image 3)
Western Blotting (WB) image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (ABIN1043683) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (C-Term) antibody (Image 4)