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anti-Human AKT antibody for Immunohistochemistry

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
pSer473
190
138
91
88
55
40
38
37
27
25
21
21
21
19
16
15
15
15
14
13
11
11
10
9
7
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human
1186
549
413
44
42
37
34
32
20
18
11
6
5
4
2
2
2
1
1
Host
Mouse
916
314
14
4
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
46
39
35
34
26
25
10
10
5
5
5
4
4
4
3
3
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
Application
Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
983
558
476
225
143
134
120
71
63
36
24
11
11
5
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN349614
$ 379.00
Plus shipping costs $45.00 and $21.00 dry ice
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
8.514159 ABIN2455512 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
8.514159 ABIN1532436 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
8.514159 ABIN1532434 IHC ELISA Rabbit IgG Log in to see Polyclonal 1
8.514159 ABIN2162535 IF IHC ELISA WB Rabbit IgG Tyr326 Log in to see Polyclonal 1
8.514159 ABIN1043729 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 4
8.514159 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
8.514159 ABIN1981391 FACS IHC WB Rabbit IgG AA 282-309, Thr308 Log in to see Polyclonal
8.514159 ABIN1981392 FACS IF IHC WB Rabbit IgG AA 428-457, C-Term Log in to see Polyclonal
8.514159 ABIN1981394 FACS IF IHC WB Rabbit IgG AA 115-144, N-Term Log in to see Polyclonal
8.514159 ABIN2455513 FACS IF IHC WB Mouse IgG1 Log in to see 4C11
8.514159 ABIN2455515 FACS IF IHC WB Mouse IgG1 Log in to see 1G5
8.514159 ABIN964560 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
8.514159 ABIN2455508 FACS IF IHC WB Mouse IgG1 Log in to see 4D6
8.514159 ABIN2455511 FACS IF IHC WB Mouse IgG1 Log in to see 3B4
8.514159 ABIN2455509 FACS IF IHC WB Mouse IgG1 Log in to see 3E3
8.514159 ABIN2455510 FACS IF IHC WB Mouse IgG1 Log in to see 4G4
8.514159 ABIN1531419 IF IHC ELISA WB Rabbit IgG pSer246 Log in to see Polyclonal
8.514159 ABIN1531766 IHC ELISA WB Rabbit IgG pSer473 Log in to see Polyclonal
8.514159 ABIN1531418 IHC ELISA WB Rabbit IgG pTyr474 Log in to see Polyclonal
8.514159 ABIN1531421 IHC ELISA WB Rabbit IgG pThr72 Log in to see Polyclonal

Top referenced anti-AKT antibody for Immunohistochemistry

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Application / Reactivity Human
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Cytometry by Time of Flight (CyTOF) 1 Antibodies
Dot Blot (DB) 71 Antibodies
ELISA 521 Antibodies
ELISA (Capture) 1 Antibodies
Enzyme Immunoassay (EIA) 36 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 138 Antibodies
Fluorescence Microscopy (FM) 3 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 11 Antibodies
Immunocytochemistry (ICC) 63 Antibodies
Immunofluorescence (IF) 223 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 24 Antibodies
Immunohistochemistry (IHC) 440 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 3 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 5 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pSer473
(190), (138), (91), (88), (55), (40), (38), (37), (27), (25), (21), (21), (21), (19), (16), (15), (15), (15), (14), (13), (11), (11), (10), (9), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human
(1186), (549), (413), (44), (42), (37), (34), (32), (20), (18), (11), (6), (5), (4), (2), (2), (2), (1), (1)
Host Mouse
(916), (314), (14), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(46), (39), (35), (34), (26), (25), (10), (10), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Application Immunohistochemistry (IHC), ELISA, Western Blotting (WB)
(983), (558), (476), (225), (143), (134), (120), (71), (63), (36), (24), (11), (11), (5), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 5 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN349614) Images
Cross-Reactivity Mouse (Murine), Rat (Rattus), Monkey
Characteristics Concentration Definition: by UV absorbance at 280 nm
Immunogen Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein.
Clone 17F6-B11
Isotype IgG1

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN349614) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Background Phospho AKT antibody is suitable for ELISA, immunohistochemistry, immunoprecipitation and western blotting. AKT phospho 473 is a component of the PI-3 kinase pathway and is activated by phosphorylation at Ser 473 and Thr 308. AKT is a cytoplasmic protein also known as AKT1, Protein Kinase B (PKB) and rac (related to A and C kinases). AKT is a key regulator of many signal transduction pathways. AKT Exhibits tight control over cell proliferation and cell viability. Overexpression or inappropriate activation of AKT is noted in many types of cancer. AKT mediates many of the downstream events of PI 3-kinase (a lipid kinase activated by growth factors, cytokines and insulin). PI 3-kinase recruits AKT to the membrane, where it is activated by PDK1 phosphorylation. Once phosphorylated, AKT dissociates from the membrane and phosphorylates targets in the cytoplasm and the cell nucleus. AKT has two main roles: (i) inhibition of apoptosis; (ii) promotion of proliferation. Anti-AKT pS473 (MOUSE) Monoclonal Antibody is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience, Signal Transduction research.
Synonyms: phospho AKT, RAC-PK-alpha, Protein kinase B, PKB, C-AKT, RAC-alpha serine/threonine-protein kinase, Proto-oncogene c-Akt, AKT1, AKT 1, AKT-1
Gene ID 207, 62241011
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN349614) Images back to top
Application Notes Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required.
Protocol This monoclonal antibody is suitable for ELISA,immunohistochemistry, immunoprecipitation and western blotting. Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffinembedded sections. No pre-treatment of sample is required. ELISA 1:20,000; WESTERN BLOT 1:500 - 1:3,000; IMMUNOHISTOCHEMISTRY 20 μg/ml; OTHER APPLICATIONS User Optimized
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN349614) Images back to top
Format Liquid
Concentration 1.02 mg/mL
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C

References for anti-AKT Antibody (ABIN349614)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Ito, Nishibori, Ito, Takagi, Akimoto, Kudo, Asanuma, Sai, Miyamoto, Takenaka, Yan: "mTORC1 activation triggers the unfolded protein response in podocytes and leads to nephrotic syndrome." in: Laboratory investigation; a journal of technical methods and pathology, 2011

Background publications

Lawlor, Alessi: "PKB/Akt: a key mediator of cell proliferation, survival and insulin responses?" in: Journal of cell science, Vol. 114, Issue Pt 16, pp. 2903-10, 2001

Alessi: "Discovery of PDK1, one of the missing links in insulin signal transduction. Colworth Medal Lecture." in: Biochemical Society transactions, Vol. 29, Issue Pt 2, pp. 1-14, 2001

Jones, Jakubowicz, Pitossi, Maurer, Hemmings: "Molecular cloning and identification of a serine/threonine protein kinase of the second-messenger subfamily." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 88, Issue 10, pp. 4171-5, 1991

Staal: "Molecular cloning of the akt oncogene and its human homologues AKT1 and AKT2: amplification of AKT1 in a primary human gastric adenocarcinoma." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 84, Issue 14, pp. 5034-7, 1987

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN349614) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) Immunohistochemistry of Mouse anti-AKT pS473 antibody. Tissue: human prostate tissue....
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) Immunohistochemistry using Anti-AKT pS473 monoclonal antibody shows detection of phos...
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 3)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 4)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 5)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 6)
 image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN349614) anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (pSer473) antibody (Image 7)