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anti-Human AKT antibody for Western Blotting

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
AA 445-480
186
123
84
64
54
39
32
30
26
21
21
18
17
16
15
14
12
12
12
11
10
10
10
9
9
9
8
7
6
6
6
6
6
6
5
5
5
5
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1101
615
474
61
59
54
46
35
31
29
18
9
8
5
5
3
3
3
2
2
1
1
1
1
Host
Rabbit
841
303
11
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
46
38
35
35
26
25
10
10
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
943
517
432
218
145
141
115
67
52
32
25
15
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
Options
Supplier
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Available images

Catalog No. ABIN725195
$ 307.45
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
3.0726016 ABIN117897 EIA IHC (fro) IF IHC (p) IP WB Rabbit AA 460-480, C-Term Log in to see Polyclonal 2
3.0726016 ABIN400784 EIA IF IHC (p) IP WB Mouse IgG1 Ser473 Log in to see 17F6-B11 4
3.0726016 ABIN400786 EIA IF IHC (p) IP WB Mouse IgG1 Thr308 Log in to see 18F3-H11 4
3.0726016 ABIN950327 EIA IHC (p) WB Rabbit Ig AA 289-318 Log in to see Polyclonal 4
3.0726016 ABIN950329 EIA FACS IF IHC (p) WB Rabbit Ig C-Term Log in to see Polyclonal 4
3.0726016 ABIN652283 FACS WB Rabbit Ig AA 438-468, C-Term Log in to see Polyclonal 4
3.0726016 ABIN389510 WB Rabbit Ig pTyr326 Log in to see Polyclonal 3
3.0726016 ABIN117898 EIA IF IHC (p) WB Rabbit AA 460-480, C-Term Log in to see Polyclonal 2
3.0726016 ABIN359075 EIA FACS WB Rabbit Ig C-Term, AA 446-475 Log in to see Polyclonal 1
3.0726016 ABIN389511 WB Rabbit Ig pTyr326 Log in to see Polyclonal 4
3.0726016 ABIN349614 IHC ELISA WB Mouse IgG1 pSer473 Log in to see 17F6-B11 5
3.0726016 ABIN2455512 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
3.0726016 ABIN2792186 WB Rabbit N-Term Log in to see Polyclonal 1
3.0726016 ABIN2792187 WB Rabbit N-Term Log in to see Polyclonal 1
3.0726016 ABIN1531520 ELISA WB Rabbit IgG pTyr326 Log in to see Polyclonal 2
3.0726016 ABIN1531420 ELISA WB Rabbit IgG pSer129 Log in to see Polyclonal 1
3.0726016 ABIN1043752 IP IHC ELISA WB Biotin Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
3.0726016 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region Log in to see 18F3-H11 4
3.0726016 ABIN223011 IP IHC WB Rabbit IgG Log in to see Polyclonal 2
3.0726016 ABIN252685 IHC IHC (p) WB Mouse IgG1 kappa pSer473 Log in to see 104A282 8

Top referenced anti-AKT antibody for Western Blotting

Similar anti-AKT Antibodies

Application / Reactivity Rat (Rattus)
Dot Blot (DB) 1 Antibodies
ELISA 166 Antibodies
Enzyme Immunoassay (EIA) 19 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 40 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 26 Antibodies
Immunofluorescence (IF) 107 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 216 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 89 Antibodies
Immunoprecipitation (IP) 58 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Simple Western (SimWes) 2 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope AA 445-480
(186), (123), (84), (64), (54), (39), (32), (30), (26), (21), (21), (18), (17), (16), (15), (14), (12), (12), (12), (11), (10), (10), (10), (9), (9), (9), (8), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1101), (615), (474), (61), (59), (54), (46), (35), (31), (29), (18), (9), (8), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(841), (303), (11), (4), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(46), (38), (35), (35), (26), (25), (10), (10), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(943), (517), (432), (218), (145), (141), (115), (67), (52), (32), (25), (15), (11), (11), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 3 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN725195) Images
Purification Purified by Protein A.
Immunogen KLH conjugated synthetic peptide derived from human AKT1
Isotype IgG

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN725195) Images back to top
Antigen
Alternative Name AKT1 (AKT1 Antibody Abstract)
Background

AKT1-specific substrates have been recently identified, including palladin (PALLD), which phosphorylation modulates cytoskeletal organization and cell motility, prohibitin (PHB), playing an important role in cell metabolism and proliferation, and CDKN1A, for which phosphorylation at 'Thr-145' induces its release from CDK2 and cytoplasmic relocalization. These recent findings indicate that the AKT1 isoform has a more specific role in cell motility and proliferation. Phosphorylates CLK2 thereby controlling cell survival to ionizing radiation.

Subcellular location: Cytoplasm, Nucleus


Synonyms: AKT, PKB, RAC, CWS6, PRKBA, PKB-ALPHA, RAC-ALPHA, RAC-alpha serine/threonine-protein kinase, Protein kinase B, Protein kinase B alpha, PKB alpha, Proto-oncogene c-Akt, RAC-PK-alpha, AKT1, RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG
Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN725195) Images back to top
Application Notes WB(1:100-1000), IHC-P(1:100-500), IF(IHC-P)(1:50-200)
Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN725195) Images back to top
Format Liquid
Concentration 1 μg/μL
Buffer Aqueous buffered solution containing 1 % BSA, 50 % glycerol and 0.09 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C
Expiry Date 12 months

References for anti-AKT Antibody (ABIN725195)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Product cited in:

Zhao, Zhang, Liu, Zhang, Hao, Li, Chen, Shen, Tang, Min, Meng, Wang, Yi, Zhang: "Hydrogen Sulfide and/or Ammonia Reduces Spermatozoa Motility through AMPK/AKT Related Pathways." in: Scientific reports, Vol. 6, pp. 37884, 2016 Method employed by authors: Western Blotting (WB) (Sample species: Pig (Porcine)).

Li, Liu, Wang, Sun, Ding, Sun, Han, Wang: "Follistatin could promote the proliferation of duck primary myoblasts by activating PI3K/Akt/mTOR signalling." in: Bioscience reports, Vol. 34, Issue 5, 2014

Xu, Huang, Xie, Lin, Guo, Zhuang, Lin, Zhou, Li, Huang, Wei, Man, Zhang: "The expression of cytoglobin as a prognostic factor in gliomas: a retrospective analysis of 88 patients." in: BMC cancer, Vol. 13, pp. 247, 2013

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN725195) back to top
Supplier Images
Immunohistochemistry (IHC) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 445-480) (ABIN725195) Formalin-fixed and paraffin embedded: mouse transplant lymphoma labeled with Anti-PKB...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 445-480) (ABIN725195) Mouse placenta lysate with Anti-AKT1/3 Polyclonal Antibody Unconjugated at 1:5000 fo...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (AA 445-480) (ABIN725195) Lane 1: Mouse Placenta lysates;Lane 2: Mouse Lung lysates probed with AKT1/3 Polyclon...
'Independent Validation' Badge
Lot Number 131113
Method validated Flow Cytometry
Positive Control MDA-MB-468 cells - lower expression
Notes A strong specific signal is observed in both of the positive control cells stained with anti-AKT1 plus secondary antibody compared with isotype, secondary only and unstained cells.
Primary Antibody
  • Antigen: V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1)
  • Catalog number: ABIN725195
  • Supplier: Log in to see
  • Supplier catalog number: Log in to see
  • Lot number: 131113
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Isotype Control Antibody
  • Antibody: Rabbit IgG
  • Catalog number: Log in to see
  • Supplier: Log in to see
  • Lot number: 16
  • Dilution: 1 µg in 100 µL 1X PBS containing 0.5% BSA
Secondary Antibody
  • Antibody: Goat anti-rabbit IgG-FITC
  • Supplier: Log in to see
  • Catalog number: Log in to see
  • Lot number: 115629
  • Dilution: 1:200 in 1X PBS containing 0.5% BSA
Controls
  • Positive control: MCF-7 cells
  • Low positive control: MDA-MB-468 cells
  • Isotype control: Both cell lines treated with rabbit IgG instead of the primary antibody to confirm that primary antibody binding is specific.
  • Secondary only control: Both cell lines treated with Goat anti-rabbit IgG-FITC to confirm no background signal produced from secondary antibody alone
Protocol
  • Control cells were cultured in DMEM + 10% FBS. - Control cells were washed once with phosphate-buffered saline (PBS) and harvested with a non-enzymatic cell dissociation solution (Cellstripper, Mediatech, Inc).
  • Detached cells were washed and resuspended in 0.5 mL 1X PBS containing 0.5% BSA.
  • An equivalent amount of pre-warmed 4% paraformaldehyde was added and the cells were incubated for 10 min at 37 °C.
  • Cells were washed in 1X PBS containing 0.5% BSA and resuspended in 1 mL of ice-cold 90% methanol for 30 min on ice.
  • Cells were spun out of the methanol and washed twice by resuspending in 1X PBS containing 0.5% BSA.
  • Cells were resuspended in 100 µL 1X PBS containing 0.5% BSA: - unstained cells - secondary antibody alone - isotype control antibody + secondary antibody - primary antibody + secondary antibody
  • Cells were incubated for 30 min on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were resuspended with 1X PBS containing 0.5% BSA + 10% goat serum and incubated for 15 min at room temperature.
  • Goat anti-rabbit IgG-FITC secondary antibody (Jackson Immunoresearch) was added at a 1:200 dilution. The cells were incubated for 30 min in the dark on ice.
  • Labeled cells were washed twice in PBS containing 0.5% BSA.
  • Cells were analyzed on a FACSAria III (BD Biosciences) using a blue laser (488 nm excitation / 525 nm emission).
Experimental Notes Nothing to note.
Validation Images
Flow Cytometry validation image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Figure 1: Positive control (high expression) MCF7 cells. The red histogram is unstain...
Flow Cytometry validation image for anti-V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) (AA 445-480) antibody (ABIN725195) Figure 2: Positive control (lower expression) MDA-MB-468 cells. The red histogram is ...