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anti-Monkey AKT antibody for Flow Cytometry

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
pSer473
185
123
84
65
54
39
32
30
28
22
21
21
18
17
16
14
13
12
12
11
11
10
10
9
9
9
8
7
6
6
6
6
6
6
5
5
5
5
4
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
1119
627
487
62
58
55
46
35
33
30
18
8
8
5
5
5
3
3
2
2
1
1
1
1
Host
Mouse
846
317
11
4
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
46
38
35
35
26
25
12
12
7
7
6
5
5
5
4
4
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
948
518
433
217
156
147
115
67
53
33
26
15
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Available images

Catalog No. ABIN4278999
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
14.207506 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6
1 ABIN2455512 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
1 ABIN2455513 FACS IF IHC WB Mouse IgG1 Log in to see 4C11
1 ABIN2455508 FACS IF IHC WB Mouse IgG1 Log in to see 4D6
1 ABIN2455511 FACS IF IHC WB Mouse IgG1 Log in to see 3B4
1 ABIN2455510 FACS IF IHC WB Mouse IgG1 Log in to see 4G4
1 ABIN2455509 FACS IF IHC WB Mouse IgG1 Log in to see 3E3
1 ABIN1496557 FACS IF IHC (p) WB Mouse IgG1 Log in to see 4E10
1 ABIN1496558 FACS IF IHC (p) WB Mouse IgG1 Log in to see 4C11
1 ABIN1496556 FACS IF IHC (p) WB Mouse IgG1 Log in to see 3B4
1 ABIN1496555 FACS IF IHC (p) WB Mouse IgG1 Log in to see 3E3
1 ABIN4279007 ELISA FACS IHC IHC (p) WB Biotin Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
1 ABIN4279006 ELISA FACS ICC IF IHC IHC (p) WB Biotin Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
1 ABIN1496554 FACS IF IHC (p) WB Mouse IgG1 Log in to see 4D6
1 ABIN4279000 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
1 ABIN4880525 ELISA FACS ICC WB Mouse IgG1 AA 1-150 Log in to see 1F7E10
1 ABIN4880526 ELISA FACS IHC WB Mouse IgG1 AA 1-150 Log in to see 1F7G2
1 ABIN2615427 FACS IF IHC (p) WB Mouse IgG1 Log in to see 4G4
1 ABIN5542264 ELISA FACS ICC IHC WB Mouse IgG1 AA 1-150 Log in to see 1F7G2
1 ABIN5542263 ELISA FACS ICC IHC WB Mouse IgG1 AA 1-150 Log in to see 1F7E10

Top referenced anti-AKT antibody for Flow Cytometry

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Application / Reactivity Rat (Rattus)
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ELISA 168 Antibodies
Enzyme Immunoassay (EIA) 20 Antibodies
FLISA 1 Antibodies
Flow Cytometry (FACS) 49 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 28 Antibodies
Immunofluorescence (IF) 107 Antibodies
Immunofluorescence (fixed cells) (IF/ICC) 11 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 218 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 90 Antibodies
Immunoprecipitation (IP) 58 Antibodies
Intracellular Staining (ICS) 3 Antibodies
Simple Western (SimWes) 2 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pSer473
(185), (123), (84), (65), (54), (39), (32), (30), (28), (22), (21), (21), (18), (17), (16), (14), (13), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(1119), (627), (487), (62), (58), (55), (46), (35), (33), (30), (18), (8), (8), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Host Mouse
(846), (317), (11), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(46), (38), (35), (35), (26), (25), (12), (12), (7), (7), (6), (5), (5), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(948), (518), (433), (217), (156), (147), (115), (67), (53), (33), (26), (15), (11), (11), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Purification Protein A purified
Immunogen Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development.
Clone 17F6-B11
Isotype IgG1 kappa

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name Akt1 (AKT1 Antibody Abstract)
Background Gene Symbol: AKT1
Molecular Weight Theoretical MW: 56 kDa
Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, Thromboxane A2 Receptor Signaling, Positive Regulation of fat Cell Differentiation, VEGFR1 Specific Signals

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes Western Blot 1:500-1:3000, Flow Cytometry, ELISA 1:20000, Immunohistochemistry 20 μg/mL, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10-1:500Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Western Blot: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the B...
Flow Cytometry (FACS) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Flow Cytometry: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Ana...
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Western Blot: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Lane 1: non-phosphor...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Ana...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Ana...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Western Blot: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Lane 1: unstimulated...
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Western Blot: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A Lane 1) PDGF stimu...
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Western Blot: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Lane 1: A431 cells. ...