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anti-Monkey AKT antibody for Flow Cytometry

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
pSer473
193
138
91
88
55
40
38
37
27
25
21
21
21
21
16
15
15
15
14
13
11
11
10
9
7
6
6
6
5
5
5
5
5
5
5
4
4
4
4
4
4
4
3
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Monkey, Mouse (Murine), Rat (Rattus)
1191
552
418
44
42
40
32
27
20
18
18
6
5
4
2
2
1
1
1
Host
Mouse
918
317
14
4
1
Clonality
Monoclonal
Conjugate
This AKT antibody is un-conjugated
46
39
35
35
26
25
10
10
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
1
1
1
1
1
1
1
Application
ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
985
556
478
224
142
133
121
71
62
36
26
11
11
5
3
3
3
3
2
1
1
1
1
1
1
1
Supplier
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Catalog No. ABIN4278999
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Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
13.113617 ABIN4279004 FACS ICC IF IHC IHC (p) WB Mouse IgG1 Log in to see 4D6
1 ABIN2455512 FACS IF IHC WB Mouse IgG1 Log in to see 4E10 1
1 ABIN2455513 FACS IF IHC WB Mouse IgG1 Log in to see 4C11
1 ABIN2455510 FACS IF IHC WB Mouse IgG1 Log in to see 4G4
1 ABIN2455508 FACS IF IHC WB Mouse IgG1 Log in to see 4D6
1 ABIN2455509 FACS IF IHC WB Mouse IgG1 Log in to see 3E3
1 ABIN2455511 FACS IF IHC WB Mouse IgG1 Log in to see 3B4
1 ABIN4279007 ELISA FACS IHC IHC (p) WB Biotin Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
1 ABIN4279006 ELISA FACS ICC IF IHC IHC (p) WB Biotin Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
1 ABIN4279000 ELISA FACS ICC IF IHC IHC (p) WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11
1 ABIN4880525 ELISA FACS ICC WB Mouse IgG1 AA 1-150 Log in to see 1F7E10
1 ABIN4880526 ELISA FACS IHC WB Mouse IgG1 AA 1-150 Log in to see 1F7G2

Top referenced anti-AKT antibody for Flow Cytometry

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FLISA 1 Antibodies
Flow Cytometry (FACS) 33 Antibodies
Fluorescence Microscopy (FM) 2 Antibodies
Fluorescence Resonance Energy Transfer Microscopy (FRET) 1 Antibodies
Immunochromatography (IC) 5 Antibodies
Immunocytochemistry (ICC) 30 Antibodies
Immunofluorescence (IF) 100 Antibodies
Immunofluorescence (Paraffin-embedded Sections) (IF (p)) 26 Antibodies
Immunohistochemistry (IHC) 221 Antibodies
Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)) 1 Antibodies
Immunohistochemistry (Frozen Sections) (IHC (fro)) 2 Antibodies
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) 53 Antibodies
Immunoprecipitation (IP) 48 Antibodies
Intracellular Staining (ICS) 3 Antibodies
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General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pSer473
(193), (138), (91), (88), (55), (40), (38), (37), (27), (25), (21), (21), (21), (21), (16), (15), (15), (15), (14), (13), (11), (11), (10), (9), (7), (6), (6), (6), (5), (5), (5), (5), (5), (5), (5), (4), (4), (4), (4), (4), (4), (4), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Monkey, Mouse (Murine), Rat (Rattus)
(1191), (552), (418), (44), (42), (40), (32), (27), (20), (18), (18), (6), (5), (4), (2), (2), (1), (1), (1)
Host Mouse
(918), (317), (14), (4), (1)
Clonality (Clone)
Monoclonal   ( )
Conjugate This AKT antibody is un-conjugated
(46), (39), (35), (35), (26), (25), (10), (10), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1)
Application ELISA, Flow Cytometry (FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
(985), (556), (478), (224), (142), (133), (121), (71), (62), (36), (26), (11), (11), (5), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling Images
Purification Protein A purified
Immunogen Anti-AKT pS473 (MOUSE) Monoclonal Antibody was produced by repeated immunizations with a synthetic peptide corresponding to residues surrounding S473 of human AKT1 protein, followed by hybridoma development.
Clone 17F6-B11
Isotype IgG1 kappa

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling Images back to top
Antigen
Alternative Name Akt1 (AKT1 Antibody Abstract)
Background Gene Symbol: AKT1
Molecular Weight Theoretical MW: 56 kDa
Gene ID 207
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling Images back to top
Application Notes Western Blot 1:500-1:3000, Flow Cytometry, ELISA 1:20000, Immunohistochemistry 20 μg/mL, Immunocytochemistry/Immunofluorescence, Immunohistochemistry-Paraffin 1:10-1:500Expect a band approximately 56 kDa in size corresponding to phosphorylated AKT protein by western blotting in the appropriate cell lysate or extract. This phospho-specific monoclonal antibody reacts with human and mouse AKT pS473 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Specific conditions for reactivity should be optimized by the end user. For immunohistochemistry use formalin-fixed paraffin-embedded sections. No pre-treatment of sample is required. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Comment

The antibodies are intended for use in vitro experiments only. Our antibodies have not been tested nor are recommended for use in vivo.

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details Images back to top
Format Liquid
Buffer 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Buffer contains: 0.01 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Store at -20°C. Avoid freeze-thaw cycles.

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling back to top
Supplier Images
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Western Blot: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the B...
Flow Cytometry (FACS) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Flow Cytometry: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Analysis using the...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Ana...
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Western Blot: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Lane 1: non-phosphor...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Ana...
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunohistochemistry-Paraffin: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Ana...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Western Blot: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Lane 1: unstimulated...
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Western Blot: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - A Lane 1) PDGF stimu...
Immunofluorescence (IF) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Immunocytochemistry/Immunofluorescence: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-699...
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pSer473) (ABIN4278999) Western Blot: Akt1 [p Ser473] Antibody (17F6.B11) [NBP1-69923] - Lane 1: A431 cells. ...