You are viewing an incomplete version of our website. Please click to reload the website as full version.

anti-Mouse (Murine) AKT antibody for ELISA

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
pTyr326
186
123
84
64
54
38
32
30
26
21
21
18
17
16
15
14
13
12
12
11
10
10
10
9
9
9
8
7
6
6
6
6
6
6
5
5
5
5
4
4
4
4
4
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1101
615
474
61
59
54
46
35
31
29
18
9
8
5
5
3
3
3
2
2
1
1
1
1
Host
Rabbit
841
303
11
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
46
38
35
35
26
25
10
10
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
ELISA, Western Blotting (WB)
943
516
432
218
146
141
115
67
52
32
26
15
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
Options
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Get this product for free

It's quick and easy to submit your validation proposal. I want to validate this product

Learn more

Available images

Catalog No. ABIN1531520
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1531420 ELISA WB Rabbit IgG pSer129 Log in to see Polyclonal 1
1 ABIN1043756 FM FRET STED ELISA WB Atto 594 Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
1 ABIN1043752 IP IHC ELISA WB Biotin Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
1 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region Log in to see 18F3-H11 4
1 ABIN1532434 IHC ELISA Rabbit IgG Log in to see Polyclonal 1
1 ABIN1533695 ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1532436 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1043730 IP IHC ELISA WB Mouse IgG1 kappa C-Term Log in to see 18F10-E5 4
1 ABIN1043729 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 4
1 ABIN968951 ELISA WB Mouse IgG1 Log in to see 3A3 3
1 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
1 ABIN1043683 FM IHC (fp) IP ELISA WB Rabbit C-Term Log in to see Polyclonal 2
1 ABIN964558 IP IHC ELISA WB Mouse IgG1 kappa C-Term Log in to see 18F10-E5
1 ABIN1450518 ELISA WB Rabbit IgG pThr308, Internal Region Log in to see Polyclonal 1
1 ABIN1450764 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 1
1 ABIN1099897 IF IHC ELISA WB Biotin Mouse IgG pSer473 Log in to see 17F6-B11
1 ABIN1099900 IHC ELISA WB Biotin Mouse IgG pThr308 Log in to see 18F3-H11
1 ABIN964560 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
1 ABIN1099896 ELISA IF IHC (p) WB Mouse IgG1 pSer473 Log in to see 17F6-B11
1 ABIN1099899 ELISA IHC (p) WB Mouse IgG pThr308 Log in to see 18F3-H11

Top referenced anti-AKT antibody for ELISA

Similar anti-AKT Antibodies

Application / Reactivity Human Mouse (Murine) Rat (Rattus)
Immunofluorescence (fixed cells) (IF/ICC) 15 Antibodies 12 Antibodies 11 Antibodies
Cytometry by Time of Flight (CyTOF) 1 Antibodies
Simple Western (SimWes) 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 888 Antibodies 530 Antibodies 411 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies 1 Antibodies 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Luminex Assay (LMNX) 3 Antibodies
Intracellular Staining (ICS) 11 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pTyr326
(186), (123), (84), (64), (54), (38), (32), (30), (26), (21), (21), (18), (17), (16), (15), (14), (13), (12), (12), (11), (10), (10), (10), (9), (9), (9), (8), (7), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (4), (4), (4), (4), (4), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1101), (615), (474), (61), (59), (54), (46), (35), (31), (29), (18), (9), (8), (5), (5), (3), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(841), (303), (11), (4), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(46), (38), (35), (35), (26), (25), (10), (10), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(943), (516), (432), (218), (146), (141), (115), (67), (52), (32), (26), (15), (11), (11), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
Supplier Log in to see

Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN1531520) Images
Specificity Akt (Phospho-Tyr326) Antibody detects endogenous levels of Akt only when phosphorylated at Tyr326.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Tyr326.
Isotype IgG

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN1531520) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Background Molecular weight of target protein: 55 kDa
Synonyms: AKT, AKT1 kinase, C-AKT, kinase Akt1, PKB, PKB-alpha, Protein kinase B, RAC, RAC-alpha serine/threonine kinase, RAC-PK-alpha
Molecular Weight 55 kDa
Gene ID 207
OMIM 164730
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Skeletal Muscle Fiber Development, Regulation of Carbohydrate Metabolic Process, Hepatitis C, Protein targeting to Nucleus

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN1531520) Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.525622 (NCBI Gene Symbol: AKT1)

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN1531520) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-AKT Antibody (ABIN1531520)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Matsubara, Wasson, Donelan, Welling, Glaser, Permutt: "Isolation and characterization of the human AKT1 gene, identification of 13 single nucleotide polymorphisms (SNPs), and their lack of association with Type II diabetes." in: Diabetologia, Vol. 44, Issue 7, pp. 910-3, 2001

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN1531520) back to top
Supplier Images
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pTyr326) (ABIN1531520) Western blot analysis of extracts from mouse liver, using Akt (Phospho-Tyr326) Antibo...