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anti-Mouse (Murine) AKT antibody for ELISA

Recommended AKT Antibody (supplied by: Log in to see )

Antigen
V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
  • AKT
  • AKT1
  • pkb
  • xAct
  • v-akt
  • v-akt1
  • akt-1
  • AKT/PKB
  • Akt
  • Akt/PKB
  • Akt1
  • PKB
  • CG4006
  • D-Akt
  • DAKT1
  • DAKT1/PKB
  • DAkt
  • DAkt1
  • DPKB
  • DRAC-PK
  • DRAC-PK66
  • DRAC-PK85
  • Dakt
  • Dakt1
  • Dmel\\CG4006
  • Dpkb
  • PKB/AKT
  • PKB/Akt
  • PKB/dAKT
  • RAC
  • RacPK
  • akt
  • akt1
  • dAKT
  • dAKT/dPKB
  • dAKT1
  • dAkt
  • dAkt/PKB
  • dAkt1
  • dPKB
  • dakt
  • dakt1
  • l(3)04226
  • l(3)89Bq
  • p-Akt
  • pAkt
  • ATAKT1
  • F18A8.2
  • F18A8_2
  • K+ transporter 1
  • POTASSIUM TRANSPORTER
  • Rac
  • PKBalpha
  • CWS6
  • PKB-ALPHA
  • PRKBA
  • RAC-ALPHA
  • v-akt murine thymoma viral oncogene homolog 1
  • CG4006 gene product from transcript CG4006-RA
  • potassium channel AKT1
  • thymoma viral proto-oncogene 1
  • AKT1
  • akt1
  • Akt1
  • KT1
Epitope
pTyr326
189
131
87
71
54
38
34
34
27
21
21
19
19
15
15
14
13
12
12
11
11
10
10
9
9
9
8
8
6
6
6
6
6
6
5
5
5
5
5
4
4
4
4
3
3
3
3
3
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
1151
648
502
64
61
54
46
35
31
29
18
9
8
5
5
5
3
3
2
2
1
1
1
1
Host
Rabbit
879
314
13
4
1
Clonality
Polyclonal
Conjugate
This AKT antibody is un-conjugated
46
38
35
35
26
25
10
10
5
5
5
4
4
4
3
3
3
3
3
3
3
3
3
2
2
2
2
2
2
2
2
1
1
1
1
Application
ELISA, Western Blotting (WB)
987
553
464
229
147
144
116
71
52
32
26
14
11
11
4
3
3
3
3
2
1
1
1
1
1
1
1
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Catalog No. ABIN1531520
$ 302.50
Plus shipping costs $45.00
Relevance Score ABIN Application Conjugate Host Isotype Epitope Supplier Clonality References Details
1 ABIN1531420 ELISA WB Rabbit IgG pSer129 Log in to see Polyclonal 1
1 ABIN1043756 FM FRET STED ELISA WB Atto 594 Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
1 ABIN1043752 IP IHC ELISA WB Biotin Mouse IgG1 kappa pSer473, Internal Region Log in to see 17F6-B11 4
1 ABIN1043753 IP IHC ELISA WB Biotin Mouse IgG1 kappa pThr308, Internal Region Log in to see 18F3-H11 4
1 ABIN1532434 IHC ELISA Rabbit IgG Log in to see Polyclonal 1
1 ABIN1533695 ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1532436 IF IHC ELISA WB Rabbit IgG Log in to see Polyclonal 1
1 ABIN1043730 IP IHC ELISA WB Mouse IgG1 kappa C-Term Log in to see 18F10-E5 4
1 ABIN2162535 IF IHC ELISA WB Rabbit IgG Tyr326 Log in to see Polyclonal 1
1 ABIN1043729 IP IHC ELISA WB Mouse IgG1 kappa pThr308 Log in to see 18F3-H11 4
1 ABIN968951 ELISA WB Mouse IgG1 Log in to see 3A3 3
1 ABIN1043683 FM IHC (fp) IP ELISA WB Rabbit C-Term Log in to see Polyclonal 2
1 ABIN153477 ELISA FACS ICC IF IHC IHC (p) WB Rabbit IgG pSer473 Log in to see Polyclonal 3
1 ABIN964558 IP IHC ELISA WB Mouse IgG1 kappa C-Term Log in to see 18F10-E5
1 ABIN1450518 ELISA WB Rabbit IgG pThr308, Internal Region Log in to see Polyclonal 1
1 ABIN1450764 ELISA WB Rabbit IgG Internal Region Log in to see Polyclonal 1
1 ABIN1099897 IF IHC ELISA WB Biotin Mouse IgG pSer473 Log in to see 17F6-B11
1 ABIN1099900 IHC ELISA WB Biotin Mouse IgG pThr308 Log in to see 18F3-H11
1 ABIN964560 IP IHC ELISA WB Mouse IgG1 kappa pSer473 Log in to see 17F6-B11
1 ABIN1099896 ELISA IF IHC (p) WB Mouse IgG1 pSer473 Log in to see 17F6-B11

Top referenced anti-AKT antibody for ELISA

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Application / Reactivity Human Mouse (Murine) Rat (Rattus)
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Cytometry by Time of Flight (CyTOF) 1 Antibodies
Simple Western (SimWes) 2 Antibodies 2 Antibodies 2 Antibodies
Western Blotting (WB) 932 Antibodies 560 Antibodies 436 Antibodies
Stimulated Emission Depletion Microscopy (STED) 1 Antibodies 1 Antibodies 1 Antibodies
RNA Interference (RNAi) 1 Antibodies
Proximity Ligation Assay (PLA) 3 Antibodies
Luminex Assay (LMNX) 3 Antibodies
Intracellular Staining (ICS) 11 Antibodies

General

Antigen V-Akt Murine Thymoma Viral Oncogene Homolog 1 (AKT1) Antibodies
Epitope pTyr326
(189), (131), (87), (71), (54), (38), (34), (34), (27), (21), (21), (19), (19), (15), (15), (14), (13), (12), (12), (11), (11), (10), (10), (9), (9), (9), (8), (8), (6), (6), (6), (6), (6), (6), (5), (5), (5), (5), (5), (4), (4), (4), (4), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity Human, Mouse (Murine), Rat (Rattus)
(1151), (648), (502), (64), (61), (54), (46), (35), (31), (29), (18), (9), (8), (5), (5), (5), (3), (3), (2), (2), (1), (1), (1), (1)
Host Rabbit
(879), (314), (13), (4), (1)
Clonality
Polyclonal
Conjugate This AKT antibody is un-conjugated
(46), (38), (35), (35), (26), (25), (10), (10), (5), (5), (5), (4), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1)
Application ELISA, Western Blotting (WB)
(987), (553), (464), (229), (147), (144), (116), (71), (52), (32), (26), (14), (11), (11), (4), (3), (3), (3), (3), (2), (1), (1), (1), (1), (1), (1), (1)
Pubmed 2 references available
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Product Details anti-AKT Antibody

Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN1531520) Images
Specificity Akt (Phospho-Tyr326) Antibody detects endogenous levels of Akt only when phosphorylated at Tyr326.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation.
Purity > 95 %
Immunogen The antiserum was produced against synthesized peptide derived from human Akt around the phosphorylation site of Tyr326.
Isotype IgG

Target Details AKT

Product Details anti-AKT Antibody Application Details Handling References for anti-AKT Antibody (ABIN1531520) Images back to top
Antigen
Alternative Name Akt (AKT1 Antibody Abstract)
Background Molecular weight of target protein: 55 kDa
Synonyms: AKT, AKT1 kinase, C-AKT, kinase Akt1, PKB, PKB-alpha, Protein kinase B, RAC, RAC-alpha serine/threonine kinase, RAC-PK-alpha
Molecular Weight 55 kDa
Gene ID 207
OMIM 164730
UniProt P31749
Pathways PI3K-Akt Signaling, RTK Signaling, TCR Signaling, AMPK Signaling, Interferon-gamma Pathway, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Response to Water Deprivation, Regulation of Actin Filament Polymerization, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process

Application Details

Product Details anti-AKT Antibody Target Details AKT Handling References for anti-AKT Antibody (ABIN1531520) Images back to top
Application Notes WB: 1:500-1:1000
ELISA: 1:20000
Optimal working dilution should be determined by the investigator.
Comment

Unigene-Number: Hs.525622 (NCBI Gene Symbol: AKT1)

Restrictions For Research Use only

Handling

Product Details anti-AKT Antibody Target Details AKT Application Details References for anti-AKT Antibody (ABIN1531520) Images back to top
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 52 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage -20 °C
Storage Comment Stable at -20 °C for at least 1 year.
Expiry Date 12 months

References for anti-AKT Antibody (ABIN1531520)

Product Details anti-AKT Antibody Target Details AKT Application Details Handling Images back to top
Background publications

Olsen, Blagoev, Gnad, Macek, Kumar, Mortensen, Mann: "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks." in: Cell, Vol. 127, Issue 3, pp. 635-48, 2006

Matsubara, Wasson, Donelan, Welling, Glaser, Permutt: "Isolation and characterization of the human AKT1 gene, identification of 13 single nucleotide polymorphisms (SNPs), and their lack of association with Type II diabetes." in: Diabetologia, Vol. 44, Issue 7, pp. 910-3, 2001

Images

Product Details anti-AKT Antibody Target Details AKT Application Details Handling References for anti-AKT Antibody (ABIN1531520) back to top
Supplier Images
Western Blotting (WB) image for anti-AKT antibody (V-Akt Murine Thymoma Viral Oncogene Homolog 1) (pTyr326) (ABIN1531520) Western blot analysis of extracts from mouse liver, using Akt (Phospho-Tyr326) Antibo...